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- EMDB-41189: Baseplate of Nexin-dynein regulatory complex from Tetrahymena the... -
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Open data
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Basic information
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Title | Baseplate of Nexin-dynein regulatory complex from Tetrahymena thermophila | |||||||||
![]() | The baseplate domain of N-DRC on doublet microtubule in Tetrahymena thermophila cilia | |||||||||
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Function / homology | ![]() axonemal dynein complex assembly / inner dynein arm assembly / cilium movement / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | |||||||||
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Method | ![]() ![]() | |||||||||
![]() | Ghanaeian AG / Black CS / Yang SK / Bui KH | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Integrated modeling of the Nexin-dynein regulatory complex reveals its regulatory mechanism. Authors: Avrin Ghanaeian / Sumita Majhi / Caitlyn L McCafferty / Babak Nami / Corbin S Black / Shun Kai Yang / Thibault Legal / Ophelia Papoulas / Martyna Janowska / Melissa Valente-Paterno / Edward ...Authors: Avrin Ghanaeian / Sumita Majhi / Caitlyn L McCafferty / Babak Nami / Corbin S Black / Shun Kai Yang / Thibault Legal / Ophelia Papoulas / Martyna Janowska / Melissa Valente-Paterno / Edward M Marcotte / Dorota Wloga / Khanh Huy Bui / ![]() ![]() ![]() Abstract: Cilia are hairlike protrusions that project from the surface of eukaryotic cells and play key roles in cell signaling and motility. Ciliary motility is regulated by the conserved nexin-dynein ...Cilia are hairlike protrusions that project from the surface of eukaryotic cells and play key roles in cell signaling and motility. Ciliary motility is regulated by the conserved nexin-dynein regulatory complex (N-DRC), which links adjacent doublet microtubules and regulates and coordinates the activity of outer doublet complexes. Despite its critical role in cilia motility, the assembly and molecular basis of the regulatory mechanism are poorly understood. Here, using cryo-electron microscopy in conjunction with biochemical cross-linking and integrative modeling, we localize 12 DRC subunits in the N-DRC structure of Tetrahymena thermophila. We also find that the CCDC96/113 complex is in close contact with the DRC9/10 in the linker region. In addition, we reveal that the N-DRC is associated with a network of coiled-coil proteins that most likely mediates N-DRC regulatory activity. | |||||||||
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 466.5 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 28.2 KB 28.2 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 18 KB | Display | ![]() |
Images | ![]() | 92.7 KB | ||
Masks | ![]() | 512 MB | ![]() | |
Others | ![]() ![]() | 412.6 MB 412.6 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8tekMC ![]() 8th8C ![]() 8tidC M: atomic model generated by this map C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Map
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Annotation | The baseplate domain of N-DRC on doublet microtubule in Tetrahymena thermophila cilia | ||||||||||||||||||||
Voxel size | X=Y=Z: 1.37 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Mask #1
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Projections & Slices |
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Density Histograms |
-Half map: The half map of baseplate domain of N-DRC...
File | emd_41189_half_map_1.map | ||||||||||||
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Annotation | The half map of baseplate domain of N-DRC on doublet microtubule in Tetrahymena thermophila cilia | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: The half map of baseplate domain of N-DRC...
File | emd_41189_half_map_2.map | ||||||||||||
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Annotation | The half map of baseplate domain of N-DRC on doublet microtubule in Tetrahymena thermophila cilia | ||||||||||||
Projections & Slices |
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Density Histograms |
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Sample components
+Entire : Baseplate of Nexin-dynein regulatory complex from Tetrahymena the...
+Supramolecule #1: Baseplate of Nexin-dynein regulatory complex from Tetrahymena the...
+Macromolecule #1: Dynein regulatory complex protein 1/2 N-terminal domain-containin...
+Macromolecule #2: Coiled-coil protein, putative
+Macromolecule #3: Growth-arrest-specific microtubule-binding protein
+Macromolecule #4: Growth-arrest-specific microtubule-binding protein
+Macromolecule #5: Cilia- and flagella-associated protein 91
+Macromolecule #6: Flagella associated protein
+Macromolecule #7: Coiled-coil protein, putative
+Macromolecule #8: DUF4201 domain-containing protein
+Macromolecule #9: Flagellar associated protein
+Macromolecule #10: CFAP20
-Experimental details
-Structure determination
Method | ![]() |
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Aggregation state | cell |
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Sample preparation
Buffer | pH: 7.4 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD![]() |
Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 45.0 e/Å2 |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |