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Open data
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Basic information
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Title | Chicken CALHM1 purified from mammalian cells | |||||||||
![]() | Octameric chicken CALHM1 structure expressed in and purified from a mammalian system. | |||||||||
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![]() | taste / assembly / calcium homeostasis modulator protein / membrane protein / channel / lipid binding / large-pore channel | |||||||||
Function / homology | Calcium homeostasis modulator family / Calcium homeostasis modulator / membrane / Calcium homeostasis modulator 1![]() | |||||||||
Biological species | ![]() ![]() ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.36 Å | |||||||||
![]() | Syrjanen JL / Furukawa H | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Structure of human CALHM1 reveals key locations for channel regulation and blockade by ruthenium red. Authors: Johanna L Syrjänen / Max Epstein / Ricardo Gómez / Hiro Furukawa / ![]() Abstract: Calcium homeostasis modulator 1 (CALHM1) is a voltage-dependent channel involved in neuromodulation and gustatory signaling. Despite recent progress in the structural biology of CALHM1, insights into ...Calcium homeostasis modulator 1 (CALHM1) is a voltage-dependent channel involved in neuromodulation and gustatory signaling. Despite recent progress in the structural biology of CALHM1, insights into functional regulation, pore architecture, and channel blockade remain limited. Here we present the cryo-EM structure of human CALHM1, revealing an octameric assembly pattern similar to the non-mammalian CALHM1s and the lipid-binding pocket conserved across species. We demonstrate by MD simulations that this pocket preferentially binds a phospholipid over cholesterol to stabilize its structure and regulate the channel activities. Finally, we show that residues in the amino-terminal helix form the channel pore that ruthenium red binds and blocks. | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 95.7 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 14.4 KB 14.4 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 14.6 KB | Display | ![]() |
Images | ![]() | 61.6 KB | ||
Filedesc metadata | ![]() | 5.5 KB | ||
Others | ![]() ![]() | 20.6 MB 20.6 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8gmqMC ![]() 8gmpC ![]() 8gmrC ![]() 8s8zC ![]() 8s90C M: atomic model generated by this map C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Map
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Annotation | Octameric chicken CALHM1 structure expressed in and purified from a mammalian system. | ||||||||||||||||||||
Voxel size | X=Y=Z: 0.856 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: Half-map of octameric chicken CALHM1 structure expressed in...
File | emd_40230_half_map_1.map | ||||||||||||
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Annotation | Half-map of octameric chicken CALHM1 structure expressed in and purified from a mammalian system. | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: Half-map of octameric chicken CALHM1 structure expressed in...
File | emd_40230_half_map_2.map | ||||||||||||
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Annotation | Half-map of octameric chicken CALHM1 structure expressed in and purified from a mammalian system. | ||||||||||||
Projections & Slices |
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Density Histograms |
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Sample components
-Entire : Octameric chicken CALHM1 with lipids
Entire | Name: Octameric chicken CALHM1 with lipids |
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Components |
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-Supramolecule #1: Octameric chicken CALHM1 with lipids
Supramolecule | Name: Octameric chicken CALHM1 with lipids / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1 |
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Source (natural) | Organism: ![]() |
-Macromolecule #1: Calcium homeostasis modulator 1
Macromolecule | Name: Calcium homeostasis modulator 1 / type: protein_or_peptide / ID: 1 / Number of copies: 8 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() ![]() |
Molecular weight | Theoretical: 34.065785 KDa |
Recombinant expression | Organism: ![]() |
Sequence | String: MDKFRMVFQF LQSNQESFMS GICGIMALAS AQLYSAFDFN CPCLPRYNLA YGLGVLLVPP LILFLLGFVL NNNVSMLAEE WRRPQGQRQ KDAAVLRYMF CSMVQRAMIA PAVWVSVTLL DGKCITCAFC TSLPVEALGN ASHHGLPQGE VKRVLARIPC K EIYDGQEL ...String: MDKFRMVFQF LQSNQESFMS GICGIMALAS AQLYSAFDFN CPCLPRYNLA YGLGVLLVPP LILFLLGFVL NNNVSMLAEE WRRPQGQRQ KDAAVLRYMF CSMVQRAMIA PAVWVSVTLL DGKCITCAFC TSLPVEALGN ASHHGLPQGE VKRVLARIPC K EIYDGQEL IANEVAVRYL RCISQALGWC FVLLMTTLAF LVRSLRPCFT QAVFLKSRYW SHYIDIERKL FDETCAEHAK SF AKVCIQQ FFQGMSKDLT ATHCHPPRKA PTDAGEASEK LLGITDWDTM NIALGSAWSH PQFEK UniProtKB: Calcium homeostasis modulator 1 |
-Macromolecule #2: (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(tri...
Macromolecule | Name: (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate type: ligand / ID: 2 / Number of copies: 8 / Formula: POV |
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Molecular weight | Theoretical: 760.076 Da |
Chemical component information | ![]() ChemComp-POV: |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 7.5 |
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Vitrification | Cryogen name: ETHANE / Chamber humidity: 85 % / Chamber temperature: 288.15 K / Instrument: FEI VITROBOT MARK IV |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 60.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.4 µm / Nominal defocus min: 0.8 µm |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |