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- PDB-3mct: VACCINIA METHYLTRANSFERASE VP39 COMPLEXED WITH M3CYT AND S-ADENOS... -
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Open data
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Basic information
Entry | Database: PDB / ID: 3mct | |||||||||
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Title | VACCINIA METHYLTRANSFERASE VP39 COMPLEXED WITH M3CYT AND S-ADENOSYLHOMOCYSTEINE | |||||||||
![]() | VP39 | |||||||||
![]() | METHYLTRANSFERASE / METHYLATED CYTOSINE / RNA CAP ANALOG / POLY(A) POLYMERASE / VACCINIA / MRNA PROCESSING / TRANSCRIPTION / COMPLEX (TRANSFERASE-RNA CAP ANALOG) | |||||||||
Function / homology | ![]() regulation of mRNA 3'-end processing / 7-methylguanosine mRNA capping / translation elongation factor activity / virion component / methyltransferase cap1 / mRNA (nucleoside-2'-O-)-methyltransferase activity / RNA binding Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | ![]() | |||||||||
![]() | Hu, G. / Gershon, P.D. / Hodel, A.E. / Quiocho, F.A. | |||||||||
![]() | ![]() Title: mRNA cap recognition: dominant role of enhanced stacking interactions between methylated bases and protein aromatic side chains. Authors: Hu, G. / Gershon, P.D. / Hodel, A.E. / Quiocho, F.A. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 91.7 KB | Display | ![]() |
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PDB format | ![]() | 69.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 721.6 KB | Display | ![]() |
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Full document | ![]() | 727.8 KB | Display | |
Data in XML | ![]() | 15 KB | Display | |
Data in CIF | ![]() | 20.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1b42C ![]() 1bkyC ![]() 1eamC ![]() 1eqaC ![]() 3magC ![]() 4dcgC C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 34819.293 Da / Num. of mol.: 1 / Mutation: C-TERMINAL DELETION OF 26 RESIDUES Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() References: UniProt: P07617, polynucleotide adenylyltransferase |
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#2: Chemical | ChemComp-SAH / |
#3: Chemical | ChemComp-3MC / |
#4: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.77 Å3/Da / Density % sol: 56 % | ||||||||||||||||||||||||
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Crystal grow | pH: 6.5 / Details: pH 6.5 | ||||||||||||||||||||||||
Crystal grow | *PLUS pH: 4.5 / Method: otherDetails: used macroseeding, Hodel, A.E., (1996) Cell, 85, 247. | ||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 103 K |
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Diffraction source | Source: ![]() |
Detector | Type: SIEMENS / Detector: AREA DETECTOR / Date: Nov 17, 1998 / Details: GOBEL MIRROR |
Radiation | Monochromator: NI FILTER / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2→99 Å / Num. obs: 27130 / % possible obs: 98.8 % / Observed criterion σ(I): 2 / Redundancy: 3.3 % / Rsym value: 0.067 / Net I/σ(I): 21 |
Reflection shell | Resolution: 2→2.1 Å / Mean I/σ(I) obs: 7 / Rsym value: 0.257 |
Reflection | *PLUS Rmerge(I) obs: 0.067 |
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Processing
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Refinement | Method to determine structure: OTHER / Resolution: 2→8 Å / Data cutoff high absF: 100000 / Data cutoff low absF: 0.0001 / Cross valid method: FREE-R / σ(F): 2
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Refinement step | Cycle: LAST / Resolution: 2→8 Å
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Refine LS restraints |
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Xplor file |
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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