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基本情報
登録情報 | ![]() | |||||||||
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タイトル | Coordinates of Cryo-EM structure of the Arabidopsis thaliana PSI in state 2 (PSI-ST2) | |||||||||
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![]() | photosystem / PLANT PROTEIN / PHOTOSYNTHESIS | |||||||||
機能・相同性 | ![]() photosynthetic NADP+ reduction / photosystem I stabilization / chloroplast photosystem I / chloroplast stromal thylakoid / response to low light intensity stimulus / chloroplast membrane / response to high light intensity / chloroplast thylakoid / plastoglobule / photosynthesis, light harvesting in photosystem I ...photosynthetic NADP+ reduction / photosystem I stabilization / chloroplast photosystem I / chloroplast stromal thylakoid / response to low light intensity stimulus / chloroplast membrane / response to high light intensity / chloroplast thylakoid / plastoglobule / photosynthesis, light harvesting in photosystem I / photosystem I reaction center / photosystem I / thylakoid / chloroplast envelope / photosystem I / plastid / chlorophyll binding / photosynthetic electron transport in photosystem I / chloroplast thylakoid membrane / photosynthesis / response to cold / chloroplast / 4 iron, 4 sulfur cluster binding / electron transfer activity / oxidoreductase activity / protein stabilization / protein domain specific binding / mRNA binding / magnesium ion binding / extracellular region / nucleus / metal ion binding / plasma membrane / cytosol 類似検索 - 分子機能 | |||||||||
生物種 | ![]() ![]() | |||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.22 Å | |||||||||
![]() | Chen SJB / Wu JH / Sui SF / Zhang LX | |||||||||
資金援助 | ![]()
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![]() | ![]() タイトル: Regulatory dynamics of the higher-plant PSI-LHCI supercomplex during state transitions. 著者: Jianghao Wu / Shuaijiabin Chen / Chao Wang / Weijun Lin / Chao Huang / Chengxu Fan / Dexian Han / Dandan Lu / Xiumei Xu / SenFang Sui / Lixin Zhang / ![]() 要旨: State transition is a fundamental light acclimation mechanism of photosynthetic organisms in response to the environmental light conditions. This process rebalances the excitation energy between ...State transition is a fundamental light acclimation mechanism of photosynthetic organisms in response to the environmental light conditions. This process rebalances the excitation energy between photosystem I (PSI) and photosystem II through regulated reversible binding of the light-harvesting complex II (LHCII) to PSI. However, the structural reorganization of PSI-LHCI, the dynamic binding of LHCII, and the regulatory mechanisms underlying state transitions are less understood in higher plants. In this study, using cryoelectron microscopy we resolved the structures of PSI-LHCI in both state 1 (PSI-LHCI-ST1) and state 2 (PSI-LHCI-LHCII-ST2) from Arabidopsis thaliana. Combined genetic and functional analyses revealed novel contacts between Lhcb1 and PsaK that further enhanced the binding of the LHCII trimer to the PSI core with the known interactions between phosphorylated Lhcb2 and the PsaL/PsaH/PsaO subunits. Specifically, PsaO was absent in the PSI-LHCI-ST1 supercomplex but present in the PSI-LHCI-LHCII-ST2 supercomplex, in which the PsaL/PsaK/PsaA subunits undergo several conformational changes to strengthen the binding of PsaO in ST2. Furthermore, the PSI-LHCI module adopts a more compact configuration with shorter Mg-to-Mg distances between the chlorophylls, which may enhance the energy transfer efficiency from the peripheral antenna to the PSI core in ST2. Collectively, our work provides novel structural and functional insights into the mechanisms of light acclimation during state transitions in higher plants. | |||||||||
履歴 |
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構造の表示
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マップデータ | ![]() | 129 MB | ![]() | |
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ヘッダ (付随情報) | ![]() ![]() | 31.2 KB 31.2 KB | 表示 表示 | ![]() |
画像 | ![]() | 39.8 KB | ||
Filedesc metadata | ![]() | 9.2 KB | ||
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
関連構造データ | ![]() 8j7bMC ![]() 8j6zC ![]() 8j7aC C: 同じ文献を引用 ( M: このマップから作成された原子モデル |
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類似構造データ | 類似検索 - 機能・相同性 ![]() |
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リンク
EMDBのページ | ![]() ![]() |
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「今月の分子」の関連する項目 |
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マップ
ファイル | ![]() | ||||||||||||||||||||
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ボクセルのサイズ | X=Y=Z: 1.087 Å | ||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||
詳細 | EMDB XML:
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-添付データ
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試料の構成要素
+全体 : Cryo-EM structure of the Arabidopsis thaliana photosystem I(PSI-L...
+超分子 #1: Cryo-EM structure of the Arabidopsis thaliana photosystem I(PSI-L...
+分子 #1: Chlorophyll a-b binding protein 6, chloroplastic
+分子 #2: Photosystem I chlorophyll a/b-binding protein 2, chloroplastic
+分子 #3: Photosystem I chlorophyll a/b-binding protein 3-1, chloroplastic
+分子 #4: Chlorophyll a-b binding protein 4, chloroplastic
+分子 #5: Photosystem I P700 chlorophyll a apoprotein A1
+分子 #6: Photosystem I P700 chlorophyll a apoprotein A2
+分子 #7: Photosystem I iron-sulfur center
+分子 #8: Photosystem I reaction center subunit II-2, chloroplastic
+分子 #9: Photosystem I reaction center subunit IV A, chloroplastic
+分子 #10: Photosystem I reaction center subunit III, chloroplastic
+分子 #11: Photosystem I reaction center subunit V, chloroplastic
+分子 #12: Photosystem I reaction center subunit VI-2, chloroplastic
+分子 #13: Photosystem I reaction center subunit VIII
+分子 #14: Photosystem I reaction center subunit IX
+分子 #15: Photosystem I reaction center subunit psaK, chloroplastic
+分子 #16: Photosystem I reaction center subunit XI, chloroplastic
+分子 #17: CHLOROPHYLL B
+分子 #18: CHLOROPHYLL A
+分子 #19: (3S,5R,6S,3'S,5'R,6'S)-5,6,5',6'-DIEPOXY-5,6,5',6'- TETRAHYDRO-BE...
+分子 #20: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE
+分子 #21: (3R,3'R,6S)-4,5-DIDEHYDRO-5,6-DIHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL
+分子 #22: BETA-CAROTENE
+分子 #23: 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE
+分子 #24: CHLOROPHYLL A ISOMER
+分子 #25: PHYLLOQUINONE
+分子 #26: IRON/SULFUR CLUSTER
+分子 #27: DIGALACTOSYL DIACYL GLYCEROL (DGDG)
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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![]() | 単粒子再構成法 |
試料の集合状態 | particle |
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試料調製
緩衝液 | pH: 7.4 |
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凍結 | 凍結剤: ETHANE |
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電子顕微鏡法
顕微鏡 | FEI TITAN |
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撮影 | フィルム・検出器のモデル: GATAN K3 (6k x 4k) / 平均電子線量: 50.5 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: ![]() |
電子光学系 | 照射モード: OTHER / 撮影モード: OTHER / 最大 デフォーカス(公称値): 2.2 µm / 最小 デフォーカス(公称値): 1.2 µm |
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画像解析
初期モデル | モデルのタイプ: NONE |
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最終 再構成 | 解像度のタイプ: BY AUTHOR / 解像度: 3.22 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 使用した粒子像数: 137602 |
初期 角度割当 | タイプ: MAXIMUM LIKELIHOOD |
最終 角度割当 | タイプ: MAXIMUM LIKELIHOOD |