EMDB-30637: Cryo-EM structure of the activated spliceosome (Bact complex) at ...

基本情報

• 登録情報: データベース: EMDB / ID: EMD-30637
• タイトル: Cryo-EM structure of the activated spliceosome (Bact complex) at an atomic resolution of 2.5 angstrom
• マップデータ: overall EM map of the yeast activated spliceosome (Bact complex) at the average resolution of 2.5 angstrom

試料

• 複合体: the activated spliceosome Bact complex
  • タンパク質・ペプチド: x 43種
  • RNA: x 4種
  • リガンド: x 3種
• リガンド: x 3種

• キーワード: RNA splicing / spliceosome / Bact complex / Prp2 / Spp2 / ATPase/helicase / activation / SPLICING

機能・相同性

分子機能:

maintenance of RNA location / RES complex / protein serine/threonine phosphatase inhibitor activity / snoRNA splicing / negative regulation of protein dephosphorylation / post-mRNA release spliceosomal complex / generation of catalytic spliceosome for first transesterification step / miRNA processing / U4/U6 snRNP / Prp19 complex / 7-methylguanosine cap hypermethylation / pre-mRNA binding / U2-type catalytic step 1 spliceosome / ATP-dependent activity, acting on RNA / small nuclear ribonucleoprotein complex / SMN-Sm protein complex / spliceosomal tri-snRNP complex / U2-type spliceosomal complex / mRNA cis splicing, via spliceosome / commitment complex / U2-type prespliceosome assembly / U2-type catalytic step 2 spliceosome / U4 snRNP / U2 snRNP / U1 snRNP / U2-type prespliceosome / precatalytic spliceosome / generation of catalytic spliceosome for second transesterification step / spliceosomal complex assembly / regulation of alternative mRNA splicing, via spliceosome / mRNA 5'-splice site recognition / regulation of RNA splicing / ATPase activator activity / U5 snRNA binding / U5 snRNP / U2 snRNA binding / U6 snRNA binding / spliceosomal snRNP assembly / mRNA export from nucleus / U4/U6 x U5 tri-snRNP complex / catalytic step 2 spliceosome / RNA splicing / helicase activity / RNA polymerase II transcription regulatory region sequence-specific DNA binding / spliceosomal complex / mRNA processing / mRNA splicing, via spliceosome / nucleic acid binding / RNA helicase activity / DNA-binding transcription factor activity, RNA polymerase II-specific / RNA helicase / nuclear speck / GTPase activity / mRNA binding / GTP binding / ATP hydrolysis activity / DNA binding / RNA binding / ATP binding / nucleus / metal ion binding / cytoplasm / cytosol

ドメイン・相同性:

Pre-mRNA-splicing factor Spp2-like / Spp2/MOS2, G-patch domain / Pre-mRNA-splicing factor CWC24-like / G-patch domain / cwf21 / Torus domain / Pre-mRNA-splicing factor Cwc2, RNA recognition motif / Torus domain / mRNA splicing factor Cwf21 domain / cwf21 domain / RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain) / G-patch domain / BUD31/G10-related, conserved site / : / : / G10 protein signature 1. / G10 protein signature 2. / glycine rich nucleic binding domain / Pre-mRNA-splicing factor Cwf15/Cwc15 / HAT (Half-A-TPR) repeat / Cwf15/Cwc15 cell cycle control protein / Splicing factor 3A subunit 1 / Zinc finger C-x8-C-x5-C-x3-H type (and similar) / Pre-mRNA-splicing factor Cwc2/Slt11 / Splicing factor 3A subunit 1, conserved domain / Pre-mRNA splicing factor PRP21 like protein / Domain of unknown function DUF382 / Domain of unknown function (DUF382) / G10 protein / : / Pre-mRNA-splicing factor BUD31 / Pre-mRNA splicing factor component Cdc5p/Cef1, C-terminal / pre-mRNA splicing factor component / SWAP/Surp / SWAP/Surp superfamily / Surp module / SURP motif repeat profile. / Suppressor-of-White-APricot splicing regulator / PHF5-like / PHF5-like protein / PSP, proline-rich / PSP / proline-rich domain in spliceosome associated proteins / Splicing factor 3B subunit 5/RDS3 complex subunit 10 / Splicing factor 3B subunit 10 (SF3b10) / U2A'/phosphoprotein 32 family A, C-terminal / Zinc finger, CCCH-type superfamily / occurring C-terminal to leucine-rich repeats / Leucine-rich repeat / : / Helicase associated domain (HA2), ratchet-like / zinc finger / Pre-mRNA-splicing factor Syf1-like / Snu114, GTP-binding domain / DEAD-box helicase, OB fold / Oligonucleotide/oligosaccharide-binding (OB)-fold / Helicase-associated domain / Helicase associated domain (HA2), winged-helix / Helicase associated domain (HA2) Add an annotation / 116kDa U5 small nuclear ribonucleoprotein component, N-terminal / 116kDa U5 small nuclear ribonucleoprotein component, C-terminal / 116 kDa U5 small nuclear ribonucleoprotein component N-terminus / Small nuclear ribonucleoprotein Sm D3 / Small nuclear ribonucleoprotein Sm D2 / Small nuclear ribonucleoprotein E / Small nuclear ribonucleoprotein G / Small nuclear ribonucleoprotein F / Sm-like protein Lsm7/SmG / Myb-type HTH DNA-binding domain profile. / Like-Sm (LSM) domain containing protein, LSm4/SmD1/SmD3 / Zinc finger, CCCH-type / Zinc finger C3H1-type profile. / Sm-like protein Lsm6/SmF / LSM domain / LSM domain, eukaryotic/archaea-type / snRNP Sm proteins / Forkhead associated domain / Forkhead-associated (FHA) domain profile. / HAT (Half-A-TPR) repeat / HAT (Half-A-TPR) repeats / Myb domain / FHA domain / : / Forkhead-associated (FHA) domain / Sm domain profile. / DNA/RNA helicase, ATP-dependent, DEAH-box type, conserved site / DEAH-box subfamily ATP-dependent helicases signature. / Myb-like DNA-binding domain / LSM domain superfamily / Translation elongation factor EFG/EF2, domain IV / Elongation factor G, domain IV / Elongation factor G, domain IV / Elongation factor G C-terminus / SMAD/FHA domain superfamily / Elongation factor EFG, domain V-like / Elongation factor G C-terminus / EF-G domain III/V-like / SANT SWI3, ADA2, N-CoR and TFIIIB'' DNA-binding domains / SANT/Myb domain / Leucine-rich repeat profile.

構成要素:

: / : / Pre-mRNA-splicing factor CLF1 / : / : / Pre-mRNA-splicing factor CWC24 / SNU114 isoform 1 / : / : / : / Pre-mRNA-splicing factor CWC21 / : / BJ4_G0014900.mRNA.1.CDS.1 / : / : / : / Pre-mRNA-splicing factor CWC2 / : / RDS3 isoform 1 / : / U2 small nuclear ribonucleoprotein A' / : / MSL1 isoform 1 / Small nuclear ribonucleoprotein Sm D3 / BUD31 isoform 1 / : / Sm protein F / : / Small nuclear ribonucleoprotein E / Small nuclear ribonucleoprotein Sm D2 / HLJ1_G0022770.mRNA.1.CDS.1 / HLJ1_G0043010.mRNA.1.CDS.1 / : / : / RDS3 complex subunit 10 / Pre-mRNA-splicing factor ATP-dependent RNA helicase-like protein PRP2 / Pre-mRNA-splicing factor PRP21 / Small nuclear ribonucleoprotein G / Pre-mRNA-splicing factor SPP2 / Pre-mRNA-splicing factor CEF1 / Pre-mRNA-splicing factor CWC15 / Pre-mRNA leakage protein 1

• 生物種: Saccharomyces cerevisiae (パン酵母) / Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (パン酵母)
• 手法: 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 2.5 Å
• データ登録者: Bai R / Wan R

資金援助

中国, 1件

Organization	Grant number	国
National Natural Science Foundation of China (NSFC)	31930059	中国

• 引用: ジャーナル: Science / 年: 2021; タイトル: Mechanism of spliceosome remodeling by the ATPase/helicase Prp2 and its coactivator Spp2.; 著者: Rui Bai / Ruixue Wan / Chuangye Yan / Qi Jia / Jianlin Lei / Yigong Shi / ; 要旨: Spliceosome remodeling, executed by conserved adenosine triphosphatase (ATPase)/helicases including Prp2, enables precursor messenger RNA (pre-mRNA) splicing. However, the structural basis for the function of the ATPase/helicases remains poorly understood. Here, we report atomic structures of Prp2 in isolation, Prp2 complexed with its coactivator Spp2, and Prp2-loaded activated spliceosome and the results of structure-guided biochemical analysis. Prp2 weakly associates with the spliceosome and cannot function without Spp2, which stably associates with Prp2 and anchors on the spliceosome, thus tethering Prp2 to the activated spliceosome and allowing Prp2 to function. Pre-mRNA is loaded into a featured channel between the N and C halves of Prp2, where Leu from the N half and Arg from the C half prevent backward sliding of pre-mRNA toward its 5'-end. Adenosine 5'-triphosphate binding and hydrolysis trigger interdomain movement in Prp2, which drives unidirectional stepwise translocation of pre-mRNA toward its 3'-end. These conserved mechanisms explain the coupling of spliceosome remodeling to pre-mRNA splicing.

履歴

登録	2020年10月26日	-
ヘッダ(付随情報) 公開	2021年3月17日	-
マップ公開	2021年3月17日	-
更新	2024年3月27日	-
現状	2024年3月27日	処理サイト: PDBj / 状態: 公開

マップ

• ファイル: ダウンロード / ファイル: emd_30637.map.gz / 形式: CCP4 / 大きさ: 244.1 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• 注釈: overall EM map of the yeast activated spliceosome (Bact complex) at the average resolution of 2.5 angstrom
• ボクセルのサイズ: X=Y=Z: 1.06 Å

密度

表面レベル	登録者による: 0.015 / ムービー #1: 0.015
最小 - 最大	-0.119457714 - 0.25123748
平均 (標準偏差)	-0.00001784702 (±0.005586911)

• 対称性: 空間群: 1

詳細

EMDB XML:

マップ形状	Axis order X Y Z Origin 0 0 0 サイズ 400 400 400 Spacing 400 400 400
セル	A=B=C: 423.99997 Å α=β=γ: 90.0 °

CCP4マップ ヘッダ情報:

mode	Image stored as Reals
Å/pix. X/Y/Z	1.06	1.06	1.06
M x/y/z	400	400	400
origin x/y/z	0.000	0.000	0.000
length x/y/z	424.000	424.000	424.000
α/β/γ	90.000	90.000	90.000
MAP C/R/S	1	2	3
start NC/NR/NS	0	0	0
NC/NR/NS	400	400	400
D min/max/mean	-0.119	0.251	-0.000

添付データ

追加マップ: EM map of Prp2 region in the Bact complex

• ファイル: emd_30637_additional_1.map
• 注釈: EM map of Prp2 region in the Bact complex

試料の構成要素

全体 : the activated spliceosome Bact complex

• 全体: 名称: the activated spliceosome Bact complex

要素

• 複合体: the activated spliceosome Bact complex
  • タンパク質・ペプチド: PRP8 isoform 1
  • RNA: U5 snRNA
  • タンパク質・ペプチド: SNU114 isoform 1
  • タンパク質・ペプチド: BRR2 isoform 1
  • タンパク質・ペプチド: Small nuclear ribonucleoprotein Sm D3
  • タンパク質・ペプチド: BJ4_G0014900.mRNA.1.CDS.1
  • タンパク質・ペプチド: SMD1 isoform 1
  • タンパク質・ペプチド: BJ4_G0037700.mRNA.1.CDS.1
  • タンパク質・ペプチド: Small nuclear ribonucleoprotein E
  • タンパク質・ペプチド: Sm protein F
  • タンパク質・ペプチド: Small nuclear ribonucleoprotein G
  • RNA: U6 snRNA
  • RNA: pre-mRNA
  • RNA: U2 snRNA
  • タンパク質・ペプチド: HLJ1_G0053790.mRNA.1.CDS.1
  • タンパク質・ペプチド: BJ4_G0027490.mRNA.1.CDS.1
  • タンパク質・ペプチド: Small nuclear ribonucleoprotein Sm D3
  • タンパク質・ペプチド: PRP9 isoform 1
  • タンパク質・ペプチド: Pre-mRNA-splicing factor PRP21
  • タンパク質・ペプチド: PRP11 isoform 1
  • タンパク質・ペプチド: HSH155 isoform 1
  • タンパク質・ペプチド: HLJ1_G0043010.mRNA.1.CDS.1
  • タンパク質・ペプチド: RSE1 isoform 1
  • タンパク質・ペプチド: HSH49 isoform 1
  • タンパク質・ペプチド: BJ4_G0056610.mRNA.1.CDS.1
  • タンパク質・ペプチド: RDS3 complex subunit 10
  • タンパク質・ペプチド: Pre-mRNA-splicing factor CEF1
  • タンパク質・ペプチド: Pre-mRNA-processing factor 19
  • タンパク質・ペプチド: SNT309 isoform 1
  • タンパク質・ペプチド: BUD31 isoform 1
  • タンパク質・ペプチド: HLJ1_G0054350.mRNA.1.CDS.1
  • タンパク質・ペプチド: Pre-mRNA-processing protein 45
  • タンパク質・ペプチド: Pre-mRNA-splicing factor SLT11
  • タンパク質・ペプチド: Pre-mRNA-splicing factor CWC2
  • タンパク質・ペプチド: Pre-mRNA-splicing factor CWC15
  • タンパク質・ペプチド: Pre-mRNA leakage protein 1
  • タンパク質・ペプチド: SX2_G0027210.mRNA.1.CDS.1
  • タンパク質・ペプチド: Pre-mRNA-splicing factor CWC26
  • タンパク質・ペプチド: CDC40 isoform 1
  • タンパク質・ペプチド: Pre-mRNA-splicing factor CWC21
  • タンパク質・ペプチド: CWC22 isoform 1
  • タンパク質・ペプチド: Pre-mRNA-splicing factor CWC24
  • タンパク質・ペプチド: CWC27 isoform 1
  • タンパク質・ペプチド: Pre-mRNA-splicing factor SPP2
  • タンパク質・ペプチド: CLF1 isoform 1
  • タンパク質・ペプチド: SYF1 isoform 1
  • タンパク質・ペプチド: Pre-mRNA-splicing factor ATP-dependent RNA helicase-like protein PRP2
  • リガンド: INOSITOL HEXAKISPHOSPHATE
  • リガンド: GUANOSINE-5'-TRIPHOSPHATE
  • リガンド: MAGNESIUM ION
• リガンド: CALCIUM ION
• リガンド: ZINC ION
• リガンド: water

超分子 #1: the activated spliceosome Bact complex

• 超分子: 名称: the activated spliceosome Bact complex / タイプ: complex / ID: 1 / 親要素: 0 / 含まれる分子: #1-#50
• 由来(天然): 生物種: Saccharomyces cerevisiae (パン酵母)

分子 #1: PRP8 isoform 1

• 分子: 名称: PRP8 isoform 1 / タイプ: protein_or_peptide / ID: 1 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Saccharomyces cerevisiae (パン酵母)
• 分子量: 理論値: 279.867469 KDa

配列

文字列:

MSGLPPPPPG FEEDSDLALP PPPPPPPGYE IEELDNPMVP SSVNEDTFLP PPPPPPSNFE INAEEIVDFT LPPPPPPPGL DELETKAEK KVELHGKRKL DIGKDTFVTR KSRKRAKKMT KKAKRSNLYT PKAEMPPEHL RKIINTHSDM ASKMYNTDKK A FLGALKYL PHAILKLLEN MPHPWEQAKE VKVLYHTSGA ITFVNETPRV IEPVYTAQWS ATWIAMRREK RDRTHFKRMR FP PFDDDEP PLSYEQHIEN IEPLDPINLP LDSQDDEYVK DWLYDSRPLE EDSKKVNGTS YKKWSFDLPE MSNLYRLSTP LRD EVTDKN YYYLFDKKSF FNGKALNNAI PGGPKFEPLY PREEEEDYNE FNSIDRVIFR VPIRSEYKVA FPHLYNSRPR SVRI PWYNN PVSCIIQNDE EYDTPALFFD PSLNPIPHFI DNNSSLNVSN TKENGDFTLP EDFAPLLAEE EELILPNTKD AMSLY HSPF PFNRTKGKMV RAQDVALAKK WFLQHPDEEY PVKVKVSYQK LLKNYVLNEL HPTLPTNHNK TKLLKSLKNT KYFQQT TID WVEAGLQLCR QGHNMLNLLI HRKGLTYLHL DYNFNLKPTK TLTTKERKKS RLGNSFHLMR ELLKMMKLIV DTHVQFR LG NVDAFQLADG IHYILNHIGQ LTGIYRYKYK VMHQIRACKD LKHIIYYKFN KNLGKGPGCG FWQPAWRVWL NFLRGTIP L LERYIGNLIT RQFEGRSNEI VKTTTKQRLD AYYDLELRNS VMDDILEMMP ESIRQKKART ILQHLSEAWR CWKANIPWD VPGMPAPIKK IIERYIKSKA DAWVSAAHYN RERIKRGAHV EKTMVKKNLG RLTRLWIKNE QERQRQIQKN GPEITPEEAT TIFSVMVEW LESRSFSPIP FPPLTYKNDT KILVLALEDL KDVYASKVRL NASEREELAL IEEAYDNPHD TLNRIKKYLL T QRVFKPVD ITMMENYQNI SPVYSVDPLE KITDAYLDQY LWYEADQRKL FPNWIKPSDS EIPPLLVYKW TQGINNLSEI WD VSRGQSA VLLETTLGEM AEKIDFTLLN RLLRLIVDPN IADYITAKNN VVINFKDMSH VNKYGLIRGL KFASFIFQYY GLV IDLLLL GQERATDLAG PANNPNEFMQ FKSKEVEKAH PIRLYTRYLD RIYMLFHFEE DEGEELTDEY LAENPDPNFE NSIG YNNRK CWPKDSRMRL IRQDVNLGRA VFWEIQSRVP TSLTSIKWEN AFVSVYSKNN PNLLFSMCGF EVRILPRQRM EEVVS NDEG VWDLVDERTK QRTAKAYLKV SEEEIKKFDS RIRGILMASG STTFTKVAAK WNTSLISLFT YFREAIVATE PLLDIL VKG ETRIQNRVKL GLNSKMPTRF PPAVFYTPKE LGGLGMISAS HILIPASDLS WSKQTDTGIT HFRAGMTHED EKLIPTI FR YITTWENEFL DSQRVWAEYA TKRQEAIQQN RRLAFEELEG SWDRGIPRIS TLFQRDRHTL AYDRGHRIRR EFKQYSLE R NSPFWWTNSH HDGKLWNLNA YRTDVIQALG GIETILEHTL FKGTGFNSWE GLFWEKASGF EDSMQFKKLT HAQRTGLSQ IPNRRFTLWW SPTINRANVY VGFLVQLDLT GIFLHGKIPT LKISLIQIFR AHLWQKIHES IVFDICQILD GELDVLQIES VTKETVHPR KSYKMNSSAA DITMESVHEW EVSKPSLLHE TNDSFKGLIT NKMWFDVQLR YGDYDSHDIS RYVRAKFLDY T TDNVSMYP SPTGVMIGID LAYNMYDAYG NWFNGLKPLI QNSMRTIMKA NPALYVLRER IRKGLQIYQS SVQEPFLNSS NY AELFNND IKLFVDDTNV YRVTVHKTFE GNVATKAING CIFTLNPKTG HLFLKIIHTS VWAGQKRLSQ LAKWKTAEEV SAL VRSLPK EEQPKQIIVT RKAMLDPLEV HMLDFPNIAI RPTELRLPFS AAMSIDKLSD VVMKATEPQM VLFNIYDDWL DRIS SYTAF SRLTLLLRAL KTNEESAKMI LLSDPTITIK SYHLWPSFTD EQWITIESQM RDLILTEYGR KYNVNISALT QTEIK DIIL GQNIKAPSVK RQKMAELEAA RSEKQNDEEA AGASTVMKTK TINAQGEEIV VVASADYESQ TFSSKNEWRK SAIANT LLY LRLKNIYVSA DDFVEEQNVY VLPKNLLKKF IEISDVKIQV AAFIYGMSAK DHPKVKEIKT VVLVPQLGHV GSVQISN IP DIGDLPDTEG LELLGWIHTQ TEELKFMAAS EVATHSKLFA DKKRDCIDIS IFSTPGSVSL SAYNLTDEGY QWGEENKD I MNVLSEGFEP TFSTHAQLLL SDRITGNFII PSGNVWNYTF MGTAFNQEGD YNFKYGIPLE FYNEMHRPVH FLQFSELAG DEELEAEQID VFS

UniProtKB: UNIPROTKB: A0A6A5PW68

分子 #3: SNU114 isoform 1

• 分子: 名称: SNU114 isoform 1 / タイプ: protein_or_peptide / ID: 3 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Saccharomyces cerevisiae (パン酵母)
• 分子量: 理論値: 114.174008 KDa

配列

文字列:

MEGDDLFDEF GNLIGVDPFD SDEEESVLDE QEQYQTNTFE GSGNNNEIES RQLTSLGSKK ELGISLEHPY GKEVEVLMET KNTQSPQTP LVEPVTERTK LQEHTIFTQL KKNIPKTRYN RDYMLSMANI PERIINVGVI GPLHSGKTSL MDLLVIDSHK R IPDMSKNV ELGWKPLRYL DNLKQEIDRG LSIKLNGSTL LCTDLESKSR MINFLDAPGH VNFMDETAVA LAASDLVLIV ID VVEGVTF VVEQLIKQSI KNNVAMCFVI NKLDRLILDL KLPPMDAYLK LNHIIANINS FTKGNVFSPI DNNIIFASTK LGF TFTIKE FVSYYYAHSI PSSKIDDFTT RLWGSVYYHK GNFRTKPFEN VEKYPTFVEF ILIPLYKIFS YALSMEKDKL KNLL RSNFR VNLSQEALQY DPQPFLKHVL QLIFRQQTGL VDAITRCYQP FELFDNKTAH LSIPGKSTPE GTLWAHVLKT VDYGG AEWS LVRIYSGLLK RGDTVRILDT SQSESRQKRQ LHDISKTETS NEDEDEDDET PSCEVEEIGL LGGRYVYPVH EAHKGQ IVL IKGISSAYIK SATLYSVKSK EDMKQLKFFK PLDYITEAVF KIVLQPLLPR ELPKLLDALN KISKYYPGVI IKVEESG EH VILGNGELYM DCLLYDLRAS YAKIEIKISD PLTVFSESCS NESFASIPVS NSISRLGEEN LPGLSISVAA EPMDSKMI Q DLSRNTLGKG QNCLDIDGIM DNPRKLSKIL RTEYGWDSLA SRNVWSFYNG NVLINDTLPD EISPELLSKY KEQIIQGFY WAVKEGPLAE EPIYGVQYKL LSISVPSDVN IDVMKSQIIP LMKKACYVGL LTAIPILLEP IYEVDITVHA PLLPIVEELM KKRRGSRIY KTIKVAGTPL LEVRGQVPVI ESAGFETDLR LSTNGLGMCQ LYFWHKIWRK VPGDVLDKDA FIPKLKPAPI N SLSRDFVM KTRRRKGIST GGFMSNDGPT LEKYISAELY AQLRENGLVP

UniProtKB: SNU114 isoform 1

分子 #4: BRR2 isoform 1

• 分子: 名称: BRR2 isoform 1 / タイプ: protein_or_peptide / ID: 4 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Saccharomyces cerevisiae (パン酵母)
• 分子量: 理論値: 246.470266 KDa

配列

文字列:

MTEHETKDKA KKIREIYRYD EMSNKVLKVD KRFMNTSQNP QRDAEISQPK SMSGRISAKD MGQGLCNNIN KGLKENDVAV EKTGKSASL KKIQQHNTIL NSSSDFRLHY YPKDPSNVET YEQILQWVTE VLGNDIPHDL IIGTADIFIR QLKENEENED G NIEERKEK IQHELGINID SLKFNELVKL MKNITDYETH PDNSNKQAVA ILADDEKSDE EEVTEMSNNA NVLGGEINDN ED DDEEYDY NDVEVNSKKK NKRALPNIEN DIIKLSDSKT SNIESVPIYS IDEFFLQRKL RSELGYKDTS VIQDLSEKIL NDI ETLEHN PVALEQKLVD LLKFENISLA EFILKNRSTI FWGIRLAKST ENEIPNLIEK MVAKGLNDLV EQYKFRETTH SKRE LDSGD DQPQSSEAKR TKFSNPAIPP VIDLEKIKFD ESSKLMTVTK VSLPEGSFKR VKPQYDEIHI PAPSKPVIDY ELKEI TSLP DWCQEAFPSS ETTSLNPIQS KVFHAAFEGD SNMLICAPTG SGKTNIALLT VLKALSHHYN PKTKKLNLSA FKIVYI APL KALVQEQVRE FQRRLAFLGI KVAELTGDSR LSRKQIDETQ VLVSTPEKWD ITTRNSNNLA IVELVRLLII DEIHLLH DD RGPVLESIVA RTFWASKYGQ EYPRIIGLSA TLPNYEDVGR FLRVPKEGLF YFDSSFRPCP LSQQFCGIKE RNSLKKLK A MNDACYEKVL ESINEGNQII VFVHSRKETS RTATWLKNKF AEENITHKLT KNDAGSKQIL KTEAANVLDP SLRKLIESG IGTHHAGLTR SDRSLSEDLF ADGLLQVLVC TATLAWGVNL PAHTVIIKGT DVYSPEKGSW EQLSPQDVLQ MLGRAGRPRY DTFGEGIII TDQSNVQYYL SVLNQQLPIE SQFVSKLVDN LNAEVVAGNI KCRNDAVNWL AYTYLYVRML ASPMLYKVPD I SSDGQLKK FRESLVHSAL CILKEQELVL YDAENDVIEA TDLGNIASSF YINHASMDVY NRELDEHTTQ IDLFRIFSMS EE FKYVSVR YEEKRELKQL LEKAPIPIRE DIDDPLAKVN VLLQSYFSQL KFEGFALNSD IVFIHQNAGR LLRAMFEICL KRG WGHPTR MLLNLCKSAT TKMWPTNCPL RQFKTCPVEV IKRLEASTVP WGDYLQLETP AEVGRAIRSE KYGKQVYDLL KRFP KMSVT CNAQPITRSV MRFNIEIIAD WIWDMNVHGS LEPFLLMLED TDGDSILYYD VLFITPDIVG HEFTLSFTYE LKQHN QNNL PPNFFLTLIS ENWWHSEFEI PVSFNGFKLP KKFPPPTPLL ENISISTSEL GNDDFSEVFE FKTFNKIQSQ VFESLY NSN DSVFVGSGKG TGKTAMAELA LLNHWRQNKG RAVYINPSGE KIDFLLSDWN KRFSHLAGGK IINKLGNDPS LNLKLLA KS HVLLATPVQF ELLSRRWRQR KNIQSLELMI YDDAHEISQG VYGAVYETLI SRMIFIATQL EKKIRFVCLS NCLANARD F GEWAGMTKSN IYNFSPSERI EPLEINIQSF KDVEHISFNF SMLQMAFEAS AAAAGNRNSS SVFLPSRKDC MEVASAFMK FSKAIEWDML NVEEEQIVPY IEKLTDGHLR APLKHGVGIL YKGMASNDER IVKRLYEYGA VSVLLISKDC SAFACKTDEV IILGTNLYD GAEHKYMPYT INELLEMVGL ASGNDSMAGK VLILTSHNMK AYYKKFLIEP LPTESYLQYI IHDTLNNEIA N SIIQSKQD CVDWFTYSYF YRRIHVNPSY YGVRDTSPHG ISVFLSNLVE TCLNDLVESS FIEIDDTEAE VTAEVNGGDD EA TEIISTL SNGLIASHYG VSFFTIQSFV SSLSNTSTLK NMLYVLSTAV EFESVPLRKG DRALLVKLSK RLPLRFPEHT SSG SVSFKV FLLLQAYFSR LELPVDFQND LKDILEKVVP LINVVVDILS ANGYLNATTA MDLAQMLIQG VWDVDNPLRQ IPHF NNKIL EKCKEINVET VYDIMALEDE ERDEILTLTD SQLAQVAAFV NNYPNVELTY SLNNSDSLIS GVKQKITIQL TRDVE PENL QVTSEKYPFD KLESWWLVLG EVSKKELYAI KKVTLNKETQ QYELEFDTPT SGKHNLTIWC VCDSYLDADK ELSFEI NVK

UniProtKB: UNIPROTKB: A0A6A5Q2I8

分子 #5: Small nuclear ribonucleoprotein Sm D3

• 分子: 名称: Small nuclear ribonucleoprotein Sm D3 / タイプ: protein_or_peptide / ID: 5 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Saccharomyces cerevisiae (パン酵母)
• 分子量: 理論値: 11.240139 KDa

配列

文字列:

MTMNGIPVKL LNEAQGHIVS LELTTGATYR GKLVESEDSM NVQLRDVIAT EPQGAVTHMD QIFVRGSQIK FIVVPDLLKN APLFKKNSS RPMPPIRGPK RR

UniProtKB: Small nuclear ribonucleoprotein Sm D3

分子 #6: BJ4_G0014900.mRNA.1.CDS.1

• 分子: 名称: BJ4_G0014900.mRNA.1.CDS.1 / タイプ: protein_or_peptide / ID: 6 / コピー数: 2 / 光学異性体: LEVO
• 由来(天然): 生物種: Saccharomyces cerevisiae (パン酵母)
• 分子量: 理論値: 22.42699 KDa

配列

文字列:

MSKIQVAHSS RLANLIDYKL RVLTQDGRVY IGQLMAFDKH MNLVLNECIE ERVPKTQLDK LRPRKDSKDG TTLNIKVEKR VLGLTILRG EQILSTVVED KPLLSKKERL VRDKKEKKQA QKQTKLRKEK EKKPGKIAKP NTANAKHTSS NSREIAQPSS S RYNGGNDN IGANRSRFNN EAPPQTRKFQ PPPGFKRK

UniProtKB: BJ4_G0014900.mRNA.1.CDS.1

分子 #7: SMD1 isoform 1

• 分子: 名称: SMD1 isoform 1 / タイプ: protein_or_peptide / ID: 7 / コピー数: 2 / 光学異性体: LEVO
• 由来(天然): 生物種: Saccharomyces cerevisiae (パン酵母)
• 分子量: 理論値: 16.296798 KDa

配列

文字列:

MKLVNFLKKL RNEQVTIELK NGTTVWGTLQ SVSPQMNAIL TDVKLTLPQP RLNKLNSNGI AMASLYLTGG QQPTASDNIA SLQYINIRG NTIRQIILPD SLNLDSLLVD QKQLNSLRRS GQIANDPSKK RRRDFGAPAN KRPRRGL

UniProtKB: UNIPROTKB: A0A6A5Q0W7

分子 #8: BJ4_G0037700.mRNA.1.CDS.1

• 分子: 名称: BJ4_G0037700.mRNA.1.CDS.1 / タイプ: protein_or_peptide / ID: 8 / コピー数: 2 / 光学異性体: LEVO
• 由来(天然): 生物種: Saccharomyces cerevisiae (パン酵母)
• 分子量: 理論値: 12.876066 KDa

配列

文字列:

MSSQIIDRPK HELSRAELEE LEEFEFKHGP MSLINDAMVT RTPVIISLRN NHKIIARVKA FDRHCNMVLE NVKELWTEKK GKNVINRER FISKLFLRGD SVIVVLKTPV E

UniProtKB: Small nuclear ribonucleoprotein Sm D2

分子 #9: Small nuclear ribonucleoprotein E

• 分子: 名称: Small nuclear ribonucleoprotein E / タイプ: protein_or_peptide / ID: 9 / コピー数: 2 / 光学異性体: LEVO
• 由来(天然): 生物種: Saccharomyces cerevisiae (パン酵母)
• 分子量: 理論値: 10.385098 KDa

配列

文字列:

MSNKVKTKAM VPPINCIFNF LQQQTPVTIW LFEQIGIRIK GKIVGFDEFM NVVIDEAVEI PVNSADGKED VEKGTPLGKI LLKGDNITL ITSAD

UniProtKB: Small nuclear ribonucleoprotein E

分子 #10: Sm protein F

• 分子: 名称: Sm protein F / タイプ: protein_or_peptide / ID: 10 / コピー数: 2 / 光学異性体: LEVO
• 由来(天然): 生物種: Saccharomyces cerevisiae (パン酵母)
• 分子量: 理論値: 9.669945 KDa

配列

文字列:

MSESSDISAM QPVNPKPFLK GLVNHRVGVK LKFNSTEYRG TLVSTDNYFN LQLNEAEEFV AGVSHGTLGE IFIRCNNVLY IRELPN

UniProtKB: Sm protein F

分子 #11: Small nuclear ribonucleoprotein G

• 分子: 名称: Small nuclear ribonucleoprotein G / タイプ: protein_or_peptide / ID: 11 / コピー数: 2 / 光学異性体: LEVO
• 由来(天然): 生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (パン酵母); 株: ATCC 204508 / S288c
• 分子量: 理論値: 8.490809 KDa

配列

文字列:

MVSTPELKKY MDKKILLNIN GSRKVAGILR GYDIFLNVVL DDAMEINGED PANNHQLGLQ TVIRGNSIIS LEALDAI

UniProtKB: Small nuclear ribonucleoprotein G

分子 #15: HLJ1_G0053790.mRNA.1.CDS.1

• 分子: 名称: HLJ1_G0053790.mRNA.1.CDS.1 / タイプ: protein_or_peptide / ID: 15 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Saccharomyces cerevisiae (パン酵母)
• 分子量: 理論値: 27.232252 KDa

配列

文字列:

MKFTPSIVID APQYYVDHFN GKYNVDKCVI LRDLQLETDS ESMPSSLKHL TKPTHILDLT NNDLIMIPDL SRRDDIHTLL LGRNNIVEV DGRLLPMNVQ NLTLSNNSIR RFEDLQRLRR APRTLKNLTL IGNQVCHLAN YREHVLRLVP HLETLDFQNV T AEERKSAM SFPRQADGDT LGPVNTAIRD NGSRDKTMEI MNLVVSKMTV ERRNELKKQL AEATSLEEIA RLEKLLSGGV

UniProtKB: U2 small nuclear ribonucleoprotein A'

分子 #16: BJ4_G0027490.mRNA.1.CDS.1

• 分子: 名称: BJ4_G0027490.mRNA.1.CDS.1 / タイプ: protein_or_peptide / ID: 16 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Saccharomyces cerevisiae (パン酵母)
• 分子量: 理論値: 12.850944 KDa

配列

文字列:

MVEPARKKQR IDRDTHHTVA EPVTEAKNTL YVSQLNEKIN MQRLRVNLFL LFATFGEVLK VSMNFKKQRG QAFITMRTID QASLAQISL NGERFFGKPL KVEFSKSETK TL

UniProtKB: MSL1 isoform 1

分子 #17: Small nuclear ribonucleoprotein Sm D3

• 分子: 名称: Small nuclear ribonucleoprotein Sm D3 / タイプ: protein_or_peptide / ID: 17 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Saccharomyces cerevisiae (パン酵母)
• 分子量: 理論値: 8.882204 KDa

配列

文字列:

NGIPVKLLNE AQGHIVSLEL TTGATYRGKL VESEDSMNVQ LRDVIATEPQ GAVTHMDQIF VRGSQIKFIV VPDLLKNAPL F

UniProtKB: Small nuclear ribonucleoprotein Sm D3

分子 #18: PRP9 isoform 1

• 分子: 名称: PRP9 isoform 1 / タイプ: protein_or_peptide / ID: 18 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Saccharomyces cerevisiae (パン酵母)
• 分子量: 理論値: 63.126445 KDa

配列

文字列:

MNLLETRRSL LEEMEIIENA IAERIQRNPE LYYHYIQESS KVFPDTKLPR SSLIAENKIY KFKKVKRKRK QIILQQHEIN IFLRDYQEK QQTFNKINRP EETQEDDKDL PNFERKLQQL EKELKNEDEN FELDINSKKD KYALFSSSSD PSRRTNILSD R ARDLDLNE IFTRDEQYGE YMELEQFHSL WLNVIKRGDC SLLQFLDILE LFLDDEKYLL TPPMDRKNDR YMAFLLKLSK YV ETFFFKS YALLDAAAVE NLIKSDFEHS YCRGSLRSEA KGIYCPFCSR WFKTSSVFES HLVGKIHKKN ESKRRNFVYS EYK LHRYLK YLNDEFSRTR SFVERKLAFT ANERMAEMDI LTQKYEAPAY DSTEKEGAEQ VDGEQRDGQL QEEHLSGKSF DMPL GPDGL PMPYWLYKLH GLDREYRCEI CSNKVYNGRR TFERHFNEER HIYHLRCLGI EPSSVFKGIT KIKEAQELWK NMQGQ SQLT SIAAVPPKPN PSQLKVPTEL ELEEEDEEGN VMSKKVYDEL KKQGLV

UniProtKB: UNIPROTKB: A0A6A5PZ67

分子 #19: Pre-mRNA-splicing factor PRP21

• 分子: 名称: Pre-mRNA-splicing factor PRP21 / タイプ: protein_or_peptide / ID: 19 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (パン酵母); 株: ATCC 204508 / S288c
• 分子量: 理論値: 33.111512 KDa

配列

文字列:

MEPEDTQLKE DIKTTVNYIK QHGVEFENKL LEDERFSFIK KDDPLHEYYT KLMNEPTDTV SGEDNDRKSE REIARPPDFL FSQYDTGIS RRDMEVIKLT ARYYAKDKSI VEQMISKDGE ARLNFMNSSH PLHKTFTDFV AQYKRVYSFT GQEIKKSKRT I LDNCFERT QYWEFEKDKD REHDKLVELC KIQFAAIPWD KFTQVAKFSI PEDTEIFEGS LDLEQMRLRR VQTGIKLFDS IK PTNEEEK IVSDQGKQKG GDSKGKKRKI RAVGETRLKK SKK

UniProtKB: Pre-mRNA-splicing factor PRP21

分子 #20: PRP11 isoform 1

• 分子: 名称: PRP11 isoform 1 / タイプ: protein_or_peptide / ID: 20 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Saccharomyces cerevisiae (パン酵母)
• 分子量: 理論値: 29.962809 KDa

配列

文字列:

MNYLEGVGSK KGGGGIASES QFNLQRRKEV ESLLSKGENV PYTFQDEKDD QVRSNPYIYK NHSGKLVCKL CNTMHMSWSS VERHLGGKK HGLNVLRRGI SIEKSSLGRE GQTTHDFRQQ QKIIEAKQSL KNNGTIPVCK IATVKNPKNG SVGLAIQVNY S SEVKENSV DSDDKAKVPP LIRIVSGLEL SDTKQKGKKF LVIAYEPFEN IAIELPPNEI LFSENNDMDN NNDGVDELNK KC TFWDAIS KLYYVQFFFK QAEQEQADV

UniProtKB: UNIPROTKB: A0A6A5Q100

分子 #21: HSH155 isoform 1

• 分子: 名称: HSH155 isoform 1 / タイプ: protein_or_peptide / ID: 21 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Saccharomyces cerevisiae (パン酵母)
• 分子量: 理論値: 110.166672 KDa

配列

文字列:

MSHPIQFVNA NNSDKSHQLG GQYSIPQDLR ENLQKEAARI GENEKDVLQE KMETRTVQNR EDSYHKRRFD MKFEPDSDTQ TVTSSENTQ DAVVPRKRKS RWDVKGYEPP DESSTAVKEN SDSALVNVEG IHDLMFFKPS DHKYFADVIS KKPIDELNKD E KKERTLSM LLLKIKNGNT ASRRTSMRIL TDKAVTFGPE MIFNRLLPIL LDRSLEDQER HLMIKTIDRV LYQLGDLTKP YV HKILVVA APLLIDEDPM VRSTGQEIIT NLSTVAGLKT ILTVMRPDIE NEDEYVRNVT SRAAAVVAKA LGVNQLLPFI NAA CHSRKS WKARHTGIKI VQQIGILLGI GVLNHLTGLM SCIKDCLMDD HVPVRIVTAH TLSTLAENSY PYGIEVFNVV LEPL WKGIR SHRGKVLSSF LKAVGSMIPL MDPEYAGYYT TEAMRIIRRE FDSPDDEMKK TILLVLQKCS AVESITPKFL REEIA PEFF QKFWVRRVAL DRPLNKVVTY TTVTLAKKLG CSYTIDKLLT PLRDEAEPFR TMAVHAVTRT VNLLGTADLD ERLETR LID ALLIAFQEQT NSDSIIFKGF GAVTVSLDIR MKPFLAPIVS TILNHLKHKT PLVRQHAADL CAILIPVIKN CHEFEML NK LNIILYESLG EVYPEVLGSI INAMYCITSV MDLDKLQPPI NQILPTLTPI LRNKHRKVEV NTIKFVGLIG KLAPTYAP P KEWMRICFEL LELLKSTNKE IRRSANATFG FIAEAIGPHD VLVALLNNLK VQERQLRVCT AVAIGIVAKV CGPYNVLPV IMNEYTTPET NVQNGVLKAM SFMFEYIGNM SKDYIYFITP LLEDALTDRD LVHRQTASNV ITHLALNCSG TGHEDAFIHL MNLLIPNIF ETSPHAIMRI LEGLEALSQA LGPGLFMNYI WAGLFHPAKN VRKAFWRVYN NMYVMYQDAM VPFYPVTPDN N EEYIEELD LVL

UniProtKB: UNIPROTKB: A0A6A5PSM1

分子 #22: HLJ1_G0043010.mRNA.1.CDS.1

• 分子: 名称: HLJ1_G0043010.mRNA.1.CDS.1 / タイプ: protein_or_peptide / ID: 22 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Saccharomyces cerevisiae (パン酵母)
• 分子量: 理論値: 27.503838 KDa

配列

文字列:

KPSLSQLKSQ VPYPQIIEWY DCDARYPGLL ASIKCTKNVI PVPSHWQSKK EYLSGRSLLG KRPFELPDII KKTNIEQMRS TLPQGGLDG QDEKSLKEAS RARVQPKMGA LDLDYKKLHD VFFKIGANWK PDHLLCFGDV YYENRNLFEE TNWKRMVDHK R PGRISQEL RAIMNLPEGQ LPPWCMKMKD IGLPTGYPDL KIAGLNWDIT NLKGDVYGKI IPNHHSRPKK QDRNYFGALI

UniProtKB: HLJ1_G0043010.mRNA.1.CDS.1

分子 #23: RSE1 isoform 1

• 分子: 名称: RSE1 isoform 1 / タイプ: protein_or_peptide / ID: 23 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Saccharomyces cerevisiae (パン酵母)
• 分子量: 理論値: 153.956781 KDa

配列

文字列:

MWGGGKMAVV SLSPHTAKMR KLFGQASTTM AYDGLKREAE RRTRSDHNIT MVAKDDELYL YHLTLKKQTN FVHSCIGHFV DLEAGSKRE QSQLCVATET HLELYDTADG ELKLIAKFQN LFATITSMKS LDLPHSGSRA KASNWPTFLA LTSDSGNLSI V QIIMHAGA LRLKTLVNQP LTRTTLRRVS PISYMEIDPN GRCIILSSVE QNKLCFLVDY AQKLRISSPL EIIRPHMVTL DM AVVDVNF NNPCFVTLEI DNAATQLSVH LIFYVLELGL NHIVKKADYL VNPSANFVLS LPDLSRYNIT TSLSDNNYDA DYD TLFNPF VVIGFENHIL VKDMNGFFSL KVEIPKRSIT NSRHKNVTII SGIVQKLKND FFVLLQSNHG DLFKLTVSPD TNDR NRPLV QLSYFDTIQN SHQLHIFKNG YLFALSEMNN NFLFQFEKLG VEKNDFSNVL TSKDPNKSLV FEPSIKLQNL SILSQ QLNL NPSIKSQIVS DSPLSIATKH FTNNKIITLT NAVNYSNLIS TSLPPNATKL WLIPDPATTG DNNTLLFITF PKKTMI LQI DNESMEELTP DEATRSAFKL SQDTTIHTCL MGSHSIIQVC TAELRHIVPT GKSRYSNKLT WVPPAGIRIV CATSSKT QL IISLSNYELV YFKIDVSSDS LIELTTHPEL DTMPSKVAIV QDTQHADLLA IADNEGMIKI MSLKDQKEDF LTVISLQL V SEKISDMIMV RDSSIGQLNL HVGLENGVYM KFHIGDVDGS FTDIKRRFLG LKPVSLSYLR EISVSLNNEE EEEEEEDDD DEKEEEEINS SGAKWMSCVV CHSSSTWVSY TWKNVWTIRQ LKDQNMLSCS KFVNADVAIN GVCSISSSGR LNIGRVSNFP TLDNWFHVH ESSVNKQENG GGDESNEEEE DEMEEEMEML QISTFRPRTI LSFPNNPKSI LFIDNHSGKK QCRISLQIDG E CLKFGSSD HLYKILDDID CVSAAIIDFT RQADHLIICA GDKRLLTYKI LVNKDKLSFD IELLHQTEII SPIHAMLKFK NF LLTAMGS TIVLYGLGKK QLLRRSVTQT PVSITKIVSM HQWNYERLAV GDIHESVTLF IWDPAGNVFI PYVDDSVKRH VTV LKFLDE ATVIGADRYG NAWTLRSPPE CEKIMSNHDP SELSNGAIKY PLDVITLQQK LPNTYDCKFK FQLLNHFFVN DIIT DFHIL DSLSNSDRPG CIYMGLQGTV GCFIPLLSKG NVFMMGNIEN IMAEADDTFY LDYESRKKNN NMRKEDDEEE SGSVV LQGR HGIEDEIICE GSCSILGRDH QEYRSYYAPV RKVIDGDLCE NFLRLSLNEQ EFLAKNLKSV QVEDIIQTIN EVRTNY M

UniProtKB: UNIPROTKB: A0A6A5PUP5

分子 #24: HSH49 isoform 1

• 分子: 名称: HSH49 isoform 1 / タイプ: protein_or_peptide / ID: 24 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Saccharomyces cerevisiae (パン酵母)
• 分子量: 理論値: 24.534152 KDa

配列

文字列:

MNYSADSGNT VYVGNIDPRI TKEQLYELFI QINPVLRIKY PKDKVLQAYQ GYAFIEFYNQ GDAQYAIKIM NNTVRLYDRL IKVRQVTNS TGTTNLPSNI SKDMILPIAK LFIKNLADSI DSDQLVKIFN KFGKLIREPE IFYLSNGKLK CAYVYFEDFE K ADLAIKSL NNQLVANNRI TVDYAFKENG KGNAKYGDDV DRLLNKEALK HNMLK

UniProtKB: UNIPROTKB: A0A6A5Q466

分子 #25: BJ4_G0056610.mRNA.1.CDS.1

• 分子: 名称: BJ4_G0056610.mRNA.1.CDS.1 / タイプ: protein_or_peptide / ID: 25 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Saccharomyces cerevisiae (パン酵母)
• 分子量: 理論値: 12.283573 KDa

配列

文字列:

MSRHQFDLIM CLKQPGVQTG LLCEKCDGKC PICDSYVRPK RKVRVCENCS FGKQAKNCII CNLNVGVNDA FYCWECCRLG KDKDGCPRI LNLGSNRLDR HFEKKKKV

UniProtKB: RDS3 isoform 1

分子 #26: RDS3 complex subunit 10

• 分子: 名称: RDS3 complex subunit 10 / タイプ: protein_or_peptide / ID: 26 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (パン酵母); 株: ATCC 204508 / S288c
• 分子量: 理論値: 9.888207 KDa

配列

文字列:

MAEKQRQLKL QKIYKQKYIG LGDESTTREQ WQRNVRNDTL NTLQGHSASL EYVSLSRGDL SIRDTRIHLL KSMSPGYKAY LREE

UniProtKB: RDS3 complex subunit 10

分子 #27: Pre-mRNA-splicing factor CEF1

• 分子: 名称: Pre-mRNA-splicing factor CEF1 / タイプ: protein_or_peptide / ID: 27 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (パン酵母); 株: ATCC 204508 / S288c
• 分子量: 理論値: 67.837773 KDa

配列

文字列:

MPPVPIYVKG GVWTNVEDQI LKAAVQKYGT HQWSKVASLL QKKTARQSEL RWNEYLNPKL NFTEFSKEED AQLLDLAREL PNQWRTIAD MMARPAQVCV ERYNRLLESE DSGGAALSTG VTDLKAGDIN PNAETQMARP DNGDLEDEEK EMLAEARARL L NTQGKKAT RKIRERMLEE SKRIAELQKR RELKQAGINV AIKKPKKKYG TDIDYNEDIV YEQAPMPGIY DTSTEDRQIK KK FEQFERK VNRKGLDGNK DKPSKKNKDK KRKHDENEHV EKAALGESTT LTDEYKKPKL ILSAPGTKQG KVTYKKKLES KRQ KLIEAQ ATGTVLTPKE LLPHDSGQED NERSNIKSGK QLKSRIRKFL VQMFASLPSP KNDFEIVLSE DEKEEDAEIA EYEK EFENE RAMNEEDNFI EPPSQNDAPR VSLVAVPLAY STLPIPEFKN NPQSAIDNKY NLLVANAINK EPHMVPEDTV DFLKE VESR MQHITQGRTS MKIQFKTAMP PTEVLLESIQ SKVESIEQLQ RKLQHVQPLE QQNNEMCSTL CHHSLPALIE GQRKYY ADY YAYRQEIRSL EGRRKRLQAM LNSSSSI

UniProtKB: Pre-mRNA-splicing factor CEF1

分子 #28: Pre-mRNA-processing factor 19

• 分子: 名称: Pre-mRNA-processing factor 19 / タイプ: protein_or_peptide / ID: 28 / コピー数: 4 / 光学異性体: LEVO / EC番号: RING-type E3 ubiquitin transferase
• 由来(天然): 生物種: Saccharomyces cerevisiae (パン酵母)
• 分子量: 理論値: 56.629777 KDa

配列

文字列:

MLCAISGKVP RRPVLSPKSR TIFEKSLLEQ YVKDTGNDPI TNEPLSIEEI VEIVPSAQQA SLTESTNSAT LKANYSIPNL LTSLQNEWD AIMLENFKLR STLDSLTKKL STVMYERDAA KLVAAQLLME KNEDSKDLPK SSQQAVAITR EEFLQGLLQS S RDFVARGK LKAPKWPILK NLELLQAQNY SRNIKTFPYK ELNKSMYYDK WVCMCRCEDG ALHFTQLKDS KTITTITTPN PR TGGEHPA IISRGPCNRL LLLYPGNQIT ILDSKTNKVL REIEVDSANE IIYMYGHNEV NTEYFIWADN RGTIGFQSYE DDS QYIVHS AKSDVEYSSG VLHKDSLLLA LYSPDGILDV YNLSSPDQAS SRFPVDEEAK IKEVKFADNG YWMVVECDQT VVCF DLRKD VGTLAYPTYT IPEFKTGTVT YDIDDSGKNM IAYSNESNSL TIYKFDKKTK NWTKDEESAL CLQSDTADFT DMDVV CGDG GIAAILKTND SFNIVALTP

UniProtKB: UNIPROTKB: A0A6A5PQI0

分子 #29: SNT309 isoform 1

• 分子: 名称: SNT309 isoform 1 / タイプ: protein_or_peptide / ID: 29 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Saccharomyces cerevisiae (パン酵母)
• 分子量: 理論値: 20.741455 KDa

配列

文字列:

MDGLSFVDKG KIPDGYKNEI DQLVKKEFAN IKREPVHPEI RGILAKRKGA DNSVSTLTNA LYTEYLKQRN NKKRRTPDFN DDDDTLFLE EYRRKYPRID TSRYIPNESS EVSLLGIVDS YLKHQEIVLD TLLPQTVSNQ WRINNDYIRQ TCTIVEEMNI Q QRKQINDL EIYRKRL

UniProtKB: UNIPROTKB: A0A6A5Q641

分子 #30: BUD31 isoform 1

• 分子: 名称: BUD31 isoform 1 / タイプ: protein_or_peptide / ID: 30 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Saccharomyces cerevisiae (パン酵母)
• 分子量: 理論値: 18.484502 KDa

配列

文字列:

MPRIKTRRSK PAPDGFEKIK PTLTDFEIQL RDAQKDKSSK LAAKSNEQLW EIMQLHHQRS RYIYTLYYKR KAISKDLYDW LIKEKYADK LLIAKWRKTG YEKLCCLRCI QKNETNNGST CICRVPRAQL EEEARKKGTQ VSFHQCVHCG CRGCASTD

UniProtKB: BUD31 isoform 1

分子 #31: HLJ1_G0054350.mRNA.1.CDS.1

• 分子: 名称: HLJ1_G0054350.mRNA.1.CDS.1 / タイプ: protein_or_peptide / ID: 31 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Saccharomyces cerevisiae (パン酵母)
• 分子量: 理論値: 37.639734 KDa

配列

文字列:

IVNRYEKLLS QRPEWHAPWK LSRVINGHLG WVRCVAIDPV DNEWFITGSN DTTMKVWDLA TGKLKTTLAG HVMTVRDVAV SDRHPYLFS VSEDKTVKCW DLEKNQIIRD YYGHLSGVRT VSIHPTLDLI ATAGRDSVIK LWDMRTRIPV ITLVGHKGPI N QVQCTPVD PQVVSSSTDA TVRLWDVVAG KTMKVLTHHK RSVRATALHP KEFSVASACT DDIRSWGLAE GSLLTNFESE KT GIINTLS INQDDVLFAG GDNGVLSFYD YKSGHKYQSL ATREMVGSLE GERSVLCSTF DKTGLRLITG EADKSIKIWK QDE TATKES EPGLAWNPNL S

UniProtKB: HLJ1_G0022770.mRNA.1.CDS.1

分子 #32: Pre-mRNA-processing protein 45

• 分子: 名称: Pre-mRNA-processing protein 45 / タイプ: protein_or_peptide / ID: 32 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Saccharomyces cerevisiae (パン酵母)
• 分子量: 理論値: 42.548727 KDa

配列

文字列:

MFSNRLPPPK HSQGRVSTAL SSDRVEPAIL TDQIAKNVKL DDFIPKRQSN FELSVPLPTK AEIQECTART KSYIQRLVNA KLANSNNRA SSRYVTETHQ APANLLLNNS HHIEVVSKQM DPLLPRFVGK KARKVVAPTE NDEVVPVLHM DGSNDRGEAD P NEWKIPAA VSNWKNPNGY TVALERRVGK ALDNENNTIN DGFMKLSEAL ENADKKARQE IRSKMELKRL AMEQEMLAKE SK LKELSQR ARYHNGTPQT GAIVKPKKQT STVARLKELA YSQGRDVSEK IILGAAKRSE QPDLQYDSRF FTRGANASAK RHE DQVYDN PLFVQQDIES IYKTNYEKLD EAVNVKSEGA SGSHGPIQFT KAESDDKSDN YGA

UniProtKB: UNIPROTKB: A0A6A5PTY9

分子 #33: Pre-mRNA-splicing factor SLT11

• 分子: 名称: Pre-mRNA-splicing factor SLT11 / タイプ: protein_or_peptide / ID: 33 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Saccharomyces cerevisiae (パン酵母)
• 分子量: 理論値: 40.98859 KDa

配列

文字列:

MNDEINEPPP NICEQCLGDE ANIRMTKIPQ GSECKICTLP FTLYHFKTSK RSNNIIKTLI CVRCATQRNI CQCCMLDSRW HIPIQLRDH LISLVNEENV MTEEAKNDMM KRFLSLKNVK LGGAQITSDP SEADNIVDKL KNILLRATSD GPSTPLIKNT T ALYKNEKG ANEVKNLEKY ASVDISHILK KLPLNESFLK NPSTKSFFLY NIDASIPEWK ITDTVSQLLG IKKWKDGNSL SL IVNHKAK CGGLRFQSSE LGERFVSKIS ETLVTPKGLK RGVLLIDRFR IFIIPWSSGF SAASFGTNTA ENIKLSLSLN KLI QLELGL SFPTKSTDNA KNDKKKTSKK VHKDRSKKSK PRANKLTI

UniProtKB: UNIPROTKB: A0A6A5Q0Z2

分子 #34: Pre-mRNA-splicing factor CWC2

• 分子: 名称: Pre-mRNA-splicing factor CWC2 / タイプ: protein_or_peptide / ID: 34 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Saccharomyces cerevisiae (パン酵母)
• 分子量: 理論値: 29.742814 KDa

配列

文字列:

MTSWRDKSAK VQVKESELPS SIPAQTGLTF NIWYNKWSQG FAGNTRFVSP FALQPQLHSG KTRGDNDGQL FFCLFFAKGM CCLGPKCEY LHHIPDEEDI GKLALRTEVL DCFGREKFAD YREDMGGIGS FRKKNKTLYV GGIDGALNSK HLKPAQIESR I RFVFSRLG DIDRIRYVES KNCGFVKFKY QANAEFAKEA MSNQTLLLPS DKEWDDRREG TGLLVKWANE DPDPAAQKRL QE ELKLESL NMMVHLINNN TNSA

UniProtKB: Pre-mRNA-splicing factor CWC2

分子 #35: Pre-mRNA-splicing factor CWC15

• 分子: 名称: Pre-mRNA-splicing factor CWC15 / タイプ: protein_or_peptide / ID: 35 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (パン酵母); 株: ATCC 204508 / S288c
• 分子量: 理論値: 19.975195 KDa

配列

文字列:

MTTSHRPQLE ARSGAKAAAY TPTGIEHARL LPGHTTLKYR KFKEEENLRA NCAQEDRSND KSLEEAVMNE EKQDVVGSGN LQETRSEKD QKDSLQELLV TQKNKVEDKA ELEGNEQLKG GNSSRRSWRK GTAFGRHKVT KETNIKEHAT KKSASGYIND M TKSEYHQE FLHKHVR

UniProtKB: Pre-mRNA-splicing factor CWC15

分子 #36: Pre-mRNA leakage protein 1

• 分子: 名称: Pre-mRNA leakage protein 1 / タイプ: protein_or_peptide / ID: 36 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (パン酵母); 株: ATCC 204508 / S288c
• 分子量: 理論値: 23.685682 KDa

配列

文字列:

MFHRRKRPYN TRNYGHDDKK FKSQYIDIMP DFSPSGLLEL ESNNKEGIAL KHVEPQDAIS PDNYMDMLGL EARDRTMYEL VIYRKNDKD KGPWKRYDLN GRSCYLVGRE LGHSLDTDLD DRTEIVVADI GIPEETSSKQ HCVIQFRNVR GILKCYVMDL D SSNGTCLN NVVIPGARYI ELRSGDVLTL SEFEEDNDYE LIFMNV

UniProtKB: Pre-mRNA leakage protein 1

分子 #37: SX2_G0027210.mRNA.1.CDS.1

• 分子: 名称: SX2_G0027210.mRNA.1.CDS.1 / タイプ: protein_or_peptide / ID: 37 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Saccharomyces cerevisiae (パン酵母)
• 分子量: 理論値: 14.880456 KDa

配列

文字列:

NKIQQINDKE LQSGILSPHQ SWHNEYKDNA YIYIGNLNRE LTEGDILTVF SEYGVPVDVI LSRDENTGES QGFAYLKYED QRSTILAVD NLNGFKIGGR ALKIDHTFYR PKRSLQKYYE AVKEELDRD

UniProtKB: UNIPROTKB: A0A6L1ALU2

分子 #38: Pre-mRNA-splicing factor CWC26

• 分子: 名称: Pre-mRNA-splicing factor CWC26 / タイプ: protein_or_peptide / ID: 38 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Saccharomyces cerevisiae (パン酵母)
• 分子量: 理論値: 14.039636 KDa

配列

文字列:

SKYEDEKAAE REQYLKNLNM GDVQKLGINV DAHDKKKNQT ASSLTIEDPA ITFTHDKERT VKTSLLGRKL YDKPAPENRF AIMPGSRWD GVHRSNGFEE KWFAKQNEIN EKKVQSYTLQ ED

UniProtKB: UNIPROTKB: A0A6L1AYM3

分子 #39: CDC40 isoform 1

• 分子: 名称: CDC40 isoform 1 / タイプ: protein_or_peptide / ID: 39 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Saccharomyces cerevisiae (パン酵母)
• 分子量: 理論値: 52.128762 KDa

配列

文字列:

MGLVDGYDTS SDSDLNFDEG KSVHEKKNGN LHEDTSYEPS SNNIHKRKSH FTKSELKRRR KTRKGDGPWG SWSSSDDETS QASETQKED QDIFVHALAE DNLDSEQIEV EEVSHFYGKS EKDYQGRGYL YPPNDVDVDL REERISFRCY LPKKVIRNYP G HPEGTTAL KFLPKTGHLI LSGGNDHTIK IWDFYHDYEC LRDFQGHNKP IKALRFTEDC QSFLSSSFDR SVKIWDTETG KV KTRLHLN STPADVESRP TNPHEFIVGL SNSKILHYDD RVSENQGLVQ TYDHHLSSIL ALKYFPDGSK FISSSEDKTV RIW ENQINV PIKQISDTAQ HSMPFLNVHP SQNYFCAQSM DNRIYSFSLK PKYKRHPKKI FKGHSSAGYG ISLAFSGDGR YICS GDSKS RLFTWDWNTS RLLNNIKIPG NKPITQVDWH PQETSKVICS GAAGKIYVCD

UniProtKB: UNIPROTKB: A0A6A5PYD5

分子 #40: Pre-mRNA-splicing factor CWC21

• 分子: 名称: Pre-mRNA-splicing factor CWC21 / タイプ: protein_or_peptide / ID: 40 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Saccharomyces cerevisiae (パン酵母)
• 分子量: 理論値: 15.793596 KDa

配列

文字列:

MSYNGIGLKS AKGSSTSGHV QRSLASNNRR RPQGSQQQRQ QRQNAIKKAS HDKASRPLAV QKQIETHMEK REIEVQVSEL RDRLEEEET LSEEQIDKKC EALRAKLTNE WQEQQRMSSL YTPRKARLTE EQHRHE

UniProtKB: Pre-mRNA-splicing factor CWC21

分子 #41: CWC22 isoform 1

• 分子: 名称: CWC22 isoform 1 / タイプ: protein_or_peptide / ID: 41 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Saccharomyces cerevisiae (パン酵母)
• 分子量: 理論値: 67.386062 KDa

配列

文字列:

MSTATIQDED IKFQRENWEM IRSHVSPIIS NLTMDNLQES HRDLFQVNIL IGRNIICKNV VDFTLNKQNG RLIPALSALI ALLNSDIPD IGETLAKELM LMFVQQFNRK DYVSCGNILQ CLSILFLYDV IHEIVILQIL LLLLEKNSLR LVIAVMKICG W KLALVSKK THDMIWEKLR YILQTQELSS TLRESLETLF EIRQKDYKSG SQGLFILDPT SYTVHTHSYI VSDEDEANKE LG NFEKCEN FNELTMAFDT LRQKLLINNT SDTNEGSNSQ LQIYDMTSTN DVEFKKKIYL VLKSSLSGDE AAHKLLKLKI ANN LKKSVV DIIIKSSLQE STFSKFYSIL SERMITFHRS WQTAYNETFE QNYTQDIEDY ETDQLRILGK FWGHLISYEF LPMD CLKII KLTEEESCPQ GRIFIKFLFQ ELVNELGLDE LQLRLNSSKL DGMFPLEGDA EHIRYSINFF TAIGLGLLTE DMRSR LTII QEVEDAEEEE KKLREEEELE KLRKKARESQ PTQGPKIHES RLFLQKDTRE NSRSRSPFTV ETRKRARSRT PPRGSR NHR NRSRTPPARR QRHR

UniProtKB: UNIPROTKB: A0A6A5Q1E9

分子 #42: Pre-mRNA-splicing factor CWC24

• 分子: 名称: Pre-mRNA-splicing factor CWC24 / タイプ: protein_or_peptide / ID: 42 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Saccharomyces cerevisiae (パン酵母)
• 分子量: 理論値: 29.797941 KDa

配列

文字列:

MFRKRLVNKS SSDEKNQKKR QKINFSEEKL VASDEEKGSS DLMSLAKSGN SRTLQLSHEN EGKLQKKGED LDKYTLTVND DSTKEDLLN FERKELAEKA KKRRPSDDNE LVLNMSGKNK RLTKQINQPT NIRTTVLMDF QPDVCKDYKQ TGYCGYGDSC K FLHSRDDF KTGWKLNQEW NADKEDSKAV TLDLEKIPFK CTLCKEDYKS PVVTNCGHYF CGSCFAKDMK KGTKCFICHK ET HGSAKVA SDLQKMLNKR KS

UniProtKB: Pre-mRNA-splicing factor CWC24

分子 #43: CWC27 isoform 1

• 分子: 名称: CWC27 isoform 1 / タイプ: protein_or_peptide / ID: 43 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Saccharomyces cerevisiae (パン酵母)
• 分子量: 理論値: 35.08093 KDa

配列

文字列:

MSSNIEPQTT AKCILYTTKG NIAIELWAKE CPETCKRFLS MLSDGTFTNG EFKELKPTQW LMFNANSTGE YRTVAEEKNP RIRFNRDGL LGWDRRRNTW FITVLADSKH VLNDCNVFGK IVGKSIYIFR EILGGEIEAS SRDNDVKRFM YPAVLKDVEI T IPFFEDIF GSKRRLEDNE KKEQEPAKKL VKSAKVKMVY EDEQEDDDGD VQKLKPRKRM ILPAWIKDDS RSEGIKLDAS LD QPQEALI REKTELHDNV DEATTKETES QENIKEEPMD KRERETLAML SKFQERIKNK NILK

UniProtKB: UNIPROTKB: A0A6A5Q291

分子 #44: Pre-mRNA-splicing factor SPP2

• 分子: 名称: Pre-mRNA-splicing factor SPP2 / タイプ: protein_or_peptide / ID: 44 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (パン酵母); 株: ATCC 204508 / S288c
• 分子量: 理論値: 20.685377 KDa

配列

文字列:

MSKFSLKLGS KTLKKNISKK TKKKNSLQKA NLFDWDDAET ASLSHKPQSK IKIQSIDKFD LDEESSSKKK LVIKLSENAD TKKNDAPLV EYVTEKEYNE VPVEEFGDAL LRGMGWESDS EQDSKGDKTQ SRNKDVSNVS QIHPDGLGIG AKLNKAINVE E ASFMPVVK IDKITGTKVD DDKKNKS

UniProtKB: Pre-mRNA-splicing factor SPP2

分子 #45: CLF1 isoform 1

• 分子: 名称: CLF1 isoform 1 / タイプ: protein_or_peptide / ID: 45 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Saccharomyces cerevisiae (パン酵母)
• 分子量: 理論値: 82.555859 KDa

配列

文字列:

MDTLEPTAVD THVSAEQILR DVYKKGQKAR GSTNIDILDL EELREYQRRK RTEYEGYLKR NRLDMGQWIR YAQFEIEQHD MRRARSIFE RALLVDSSFI PLWIRYIDAE LKVKCINHAR NLMNRAISTL PRVDKLWYKY LIVEESLNNV EIVRSLYTKW C SLEPGVNA WNSFVDFEIR QKNWNGVREI YSKYVMAHPQ MQTWLKWVRF ENRHGNTEFT RSVYSLAIDT VANLQNLQIW SD MEVAKLV NSFAHWEAAQ QEYERSSALY QIAIEKWPSN QLLKAGLLDF EKQFGDINSI EETISYKRKM EYETILSNNA YDY DTWWLY LDLISESFPK QIMQTFEKAI VDSRPKELSK NVQWKRYIYL WMRYICYVEL ELENSLLEEE LFQRLIDDII PHKH FTFSK IWLMYAKFLI RHDDVPKARK ILGKAIGLCP KAKTFKGYIE LEVKLKEFDR VRKIYEKFIE FQPSDLQIWS QYGEL EENL GDWDRVRGIY TIALDENSDF LTKEAKIVLL QKYITFETES QEFEKARKLY RRYLELNQYS PQSWIEFAMY QTSTPT EQQ LLDLAKLQSE NVDEDIEFEI TDENKLEARK VFEEAIVFFK EKDDKQGRLS ILEALKDYEE TYGTELDQET VKKRFPK VI KKVRLQNGVE EEFVDYIFPD DIDDDKPKPS KFLELAKKWK QEQAL

UniProtKB: Pre-mRNA-splicing factor CLF1

分子 #46: SYF1 isoform 1

• 分子: 名称: SYF1 isoform 1 / タイプ: protein_or_peptide / ID: 46 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: Saccharomyces cerevisiae (パン酵母)
• 分子量: 理論値: 100.344016 KDa

配列

文字列:

MSAYIAMKGV ITNVDENIRN DEDVAFEYEI QKTPQNILTW KRYIEYWKEE GRTDKQIRWL YERFCSQFVT DTSIWEDYIR WESTKEVVE TSRIFWLFQR CLKSCVRDCD RICLSYLELA IEQYDLAMIR HALASSLMKM EREMHRKVWD PVIKFVEEKV L PLTQLDST QEDEEESTDE AELINVLLVK GFTKGGFISE EISENGSRGD IWSSHILERY LKVAPQQKRN ESLATLALTR DN ITIKSVY EKYLPQDENS GKYLPSSELP FELNFNYLAS LEKLGLDNQY EEFMRQMNGI YPDKWLFLIL SLAKYYISRG RLD SCGDLL KKSLQQTLRY SDFDRIYNFY LLFEQECSQF ILGKLKENDS KFFNQKDWTE KLQAHMATFE SLINLYDIYL NDVA LRQDS NLVETWMKRV SLQKSAAEKC NVYSEAILKI DPRKVGTPGS FGRLWCSYGD LYWRSNAIST ARELWTQSLK VPYPY IEDL EEIYLNWADR ELDKEGVERA FSILEDALHV PTNPEILLEK YKNGHRKIPA QTVLFNSLRI WSKYIDYLEA YCPKDA NSS DKIFNKTKMA YNTVIDLRLI TPAMAENFAL FLQNHYEVME SFQVYEKTIP LFPPEIQYEL WIEYLEVATS HQLSSLS PE HIRFLFEKAL KNLCSNGIDC KTIFIAYSVF EERISGLISK SIEILRRGAV IGTVSVSTHL ESRLQLWRMC ISKAESTL G PSVTRELYQE CIQILPNSKA VEFVIKFSDF ESSIGETIRA REILAYGAKL LPPSRNTELW DSFEIFELKH GDKETYKDM LKMKKVLESN MLIDSASVSH EEGNINFVAA ATSHAPNSHT LTQSTSSYSI NPDEIELDI

UniProtKB: UNIPROTKB: A0A6A5Q0A4

分子 #47: Pre-mRNA-splicing factor ATP-dependent RNA helicase-like protein PRP2

• 分子: 名称: Pre-mRNA-splicing factor ATP-dependent RNA helicase-like protein PRP2; タイプ: protein_or_peptide / ID: 47 / コピー数: 1 / 光学異性体: LEVO / EC番号: RNA helicase
• 由来(天然): 生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (パン酵母); 株: ATCC 204508 / S288c
• 分子量: 理論値: 99.947492 KDa

配列

文字列:

MSSITSETGK RRVKRTYEVT RQNDNAVRIE PSSLGEEEDK EAKDKNSALQ LKRSRYDPNK VFSNTNQGPE KNNLKGEQLG SQKKSSKYD EKITSNNELT TKKGLLGDSE NETKYASSNS KFNVEVTHKI KNAKEIDKIN RQRMWEEQQL RNAMAGQSDH P DDITLEGS DKYDYVFDTD AMIDYTNEED DLLPEEKLQY EARLAQALET EEKRILTIQE ARKLLPVHQY KDELLQEIKK NQ VLIIMGE TGSGKTTQLP QYLVEDGFTD QGKLQIAITQ PRRVAATSVA ARVADEMNVV LGKEVGYQIR FEDKTTPNKT VLK YMTDGM LLREFLTDSK LSKYSCIMID EAHERTLATD ILIGLLKDIL PQRPTLKLLI SSATMNAKKF SEFFDNCPIF NVPG RRYPV DIHYTLQPEA NYIHAAITTI FQIHTTQSLP GDILVFLTGQ EEIERTKTKL EEIMSKLGSR TKQMIITPIY ANLPQ EQQL KIFQPTPENC RKVVLATNIA ETSLTIDGIR YVIDPGFVKE NSYVPSTGMT QLLTVPCSRA SVDQRAGRAG RVGPGK CFR IFTKWSYLHE LELMPKPEIT RTNLSNTVLL LLSLGVTDLI KFPLMDKPSI PTLRKSLENL YILGALNSKG TITRLGK MM CEFPCEPEFA KVLYTAATHE QCQGVLEECL TIVSMLHETP SLFIGQKRDA AASVLSEVES DHILYLEIFN QWRNSKFS R SWCQDHKIQF KTMLRVRNIR NQLFRCSEKV GLVEKNDQAR MKIGNIAGYI NARITRCFIS GFPMNIVQLG PTGYQTMGR SSGGLNVSVH PTSILFVNHK EKAQRPSKYV LYQQLMLTSK EFIRDCLVIP KEEWLIDMVP QIFKDLIDDK TNRGRR

UniProtKB: Pre-mRNA-splicing factor ATP-dependent RNA helicase-like protein PRP2

分子 #2: U5 snRNA

• 分子: 名称: U5 snRNA / タイプ: rna / ID: 2 / コピー数: 1
• 由来(天然): 生物種: Saccharomyces cerevisiae (パン酵母)
• 分子量: 理論値: 68.643344 KDa

配列

文字列:

AAGCAGCUUU ACAGAUCAAU GGCGGAGGGA GGUCAACAUC AAGAACUGUG GGCCUUUUAU UGCCUAUAGA ACUUAUAACG AACAUGGUU CUUGCCUUUU ACCAGAACCA UCCGGGUGUU GUCUCCAUAG AAACAGGUAA AGCUGUCCGU UACUGUGGGC U UGCCAUAU UUUUUGGAAC UUUUCUGCCC UUUUUCUCAA UGAGUAAGGA GGGCGU

GENBANK: GENBANK: CP008004.1

分子 #12: U6 snRNA

• 分子: 名称: U6 snRNA / タイプ: rna / ID: 12 / コピー数: 1
• 由来(天然): 生物種: Saccharomyces cerevisiae (パン酵母)
• 分子量: 理論値: 35.883176 KDa

配列

文字列:

GUUCGCGAAG UAACCCUUCG UGGACAUUUG GUCAAUUUGA AACAAUACAG AGAUGAUCAG CAGUUCCCCU GCAUAAGGAU GAACCGUUU UACAAAGAGA UUUAUUUCGU UUU

GENBANK: GENBANK: CP007856.1

分子 #13: pre-mRNA

• 分子: 名称: pre-mRNA / タイプ: rna / ID: 13 / コピー数: 1
• 由来(天然): 生物種: Saccharomyces cerevisiae (パン酵母)
• 分子量: 理論値: 44.772172 KDa

配列

文字列:

AAAAUAAAAA AAAAAAAAUU UGUAAGGUAU GUAUUAUUUU UU(N)(N)(N)(N)(N)(N)(N)(N) (N)(N)(N)(N) (N)(N)(N)(N)(N) (N)(N)(N)(N)(N)(N)(N)(N)(N)(N) (N)(N)(N)(N)(N)(N)(N)(N)(N)(N) (N) (N)(N)(N)(N)(N)(N)(N)(N) (N)(N)(N)(N)AAAAAA AA(N)(N)(N)AAAAA A(N)AAAAACUA GAUACUAACA CAUUUAAUU UUUUUUUGUU UUU(N)(N)UUUUU UUUUU

分子 #14: U2 snRNA

• 分子: 名称: U2 snRNA / タイプ: rna / ID: 14 / コピー数: 1
• 由来(天然): 生物種: Saccharomyces cerevisiae (パン酵母)
• 分子量: 理論値: 376.324562 KDa

配列

文字列:

ACGAAUCUCU UUGCCUUUUG GCUUAGAUCA AGUGUAGUAU CUGUUCUUUU CAGUGUAACA ACUGAAAUGA CCUCAAUGAG GCUCAUUAC CUUUUAAUUU GUUACAAUAC ACAUUUUUUG GCACCCAAAA UAAUAAAAUG GACGGGAAGA GACUUUUUAA G CAAGACGU UUUCCGCUAA UGACACCUCG CACGAGUCGU UCUUGCUAUC UUUGGUCGCU UGAUGUUUCU UCUUAACCCG UU CUUAUGA UGGUUUUUCG AAAUUGGUUU UUGAGACGAC GGUUGCUCAA GGUUAUUGUU UUUGUUUUCU UCUGGUUGUU UUC UAUUUU CUUUUUUUUA GCUUUCUGUU UCUCCCUUAG UUUGGCUUUU UGCUUCAUAC UCUUCCCUGU CUUUCCGAGC CGUU UAUGU CCAACGCGGG AUUUGGUUUU UCUUUAUCGA UGGGAAGAAA UGGUGCUAUA GUAGGUUGGG AGAUAAUAUU UAUGG UAUG GGGUGCUAGU GCGGAUGGGG CGCUCUUAUU GUUGAUUUCU UCGCUCGUCU UCUUUUUCUG GUGGCGCUGC AAGAGG AAG UUUUUCGACU UUGUUAUGAU UUUUGGUUUG CAAGGAAAGG UGUCUUACGA UUCUUUUUUU GAUGUAAUAG GAUAAGC UU GCUUAUCCCC CAAGUAUCGG CCAAAGUUGU UGAUUUUCCU UUUGAAGUGU CCUCGGUUUG AGGGGGUGUA GGGUGGGG U UGGUCUACAA UAAGAGUGUU CCAUUGUUAA CGUGCUGGCG UCUUUUACUA UAUUUUUUUU CCCAGUUUAU UUUGUGCUU AUUUUCUCAU UGAGGAGAAG GAGCUCUUCU CGCAGGAUAU AAAUGGAGGU UUGCUAAAGG GGAGGAGAUG UGUUUGUGAG AAUACUGCU GAGAGAGUUC UGGAAGAGAA AAAAAGGAGG CAAUGGAAGG CGUUUGCUGG GAAAAGAGAA GAGCCAUGAC U GCAUCUGU UGUUUCAAGG CCAGUUUUAU UAACCGCCUA UGUCAUAGAG GCGUUUUUUU UGGAGGGAUU UGAAGAAUGC CG GCGGCAU CAAGAAACGG ACUUGAUGGU UGACGCCUGU UUUUAAAGUU AGAGACGUCG CGACCCUCGC ACUUGUGGAG UCG UUCUUG ACUUUUACUU UGGUCGCUUG AUGUUUCUCU CGUCUUCCCG UUCGCUCUU

分子 #48: INOSITOL HEXAKISPHOSPHATE

• 分子: 名称: INOSITOL HEXAKISPHOSPHATE / タイプ: ligand / ID: 48 / コピー数: 1 / 式: IHP
• 分子量: 理論値: 660.035 Da

Chemical component information

ChemComp-IHP:
INOSITOL HEXAKISPHOSPHATE / フィチン酸

分子 #49: GUANOSINE-5'-TRIPHOSPHATE

• 分子: 名称: GUANOSINE-5'-TRIPHOSPHATE / タイプ: ligand / ID: 49 / コピー数: 1 / 式: GTP
• 分子量: 理論値: 523.18 Da

Chemical component information

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP / GTP, エネルギー貯蔵分子*YM

分子 #50: MAGNESIUM ION

• 分子: 名称: MAGNESIUM ION / タイプ: ligand / ID: 50 / コピー数: 6 / 式: MG
• 分子量: 理論値: 24.305 Da

分子 #51: CALCIUM ION

• 分子: 名称: CALCIUM ION / タイプ: ligand / ID: 51 / コピー数: 1 / 式: CA
• 分子量: 理論値: 40.078 Da

分子 #52: ZINC ION

• 分子: 名称: ZINC ION / タイプ: ligand / ID: 52 / コピー数: 15 / 式: ZN
• 分子量: 理論値: 65.409 Da

分子 #53: water

• 分子: 名称: water / タイプ: ligand / ID: 53 / コピー数: 1714 / 式: HOH
• 分子量: 理論値: 18.015 Da

Chemical component information

ChemComp-HOH:
WATER

実験情報

構造解析

• 手法: クライオ電子顕微鏡法
• 解析: 単粒子再構成法
• 試料の集合状態: particle

試料調製

• 緩衝液: pH: 7.9
• グリッド: モデル: Quantifoil / 材質: GOLD / 支持フィルム - #0 - Film type ID: 1 / 支持フィルム - #0 - 材質: GRAPHENE / 支持フィルム - #0 - トポロジー: CONTINUOUS / 支持フィルム - #1 - Film type ID: 2 / 支持フィルム - #1 - 材質: CARBON / 支持フィルム - #1 - トポロジー: CONTINUOUS / 支持フィルム - #2 - Film type ID: 3 / 支持フィルム - #2 - トポロジー: HOLEY
• 凍結: 凍結剤: ETHANE

電子顕微鏡法

• 顕微鏡: FEI TITAN KRIOS
• 撮影: フィルム・検出器のモデル: GATAN K2 SUMMIT (4k x 4k); 検出モード: SUPER-RESOLUTION / 平均電子線量: 50.0 e/Ų
• 電子線: 加速電圧: 300 kV / 電子線源: FIELD EMISSION GUN
• 電子光学系: 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD
• 実験機器: モデル: Titan Krios / 画像提供: FEI Company

画像解析

• 初期モデル: モデルのタイプ: EMDB MAP
• 最終 再構成: 解像度のタイプ: BY AUTHOR / 解像度: 2.5 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 使用した粒子像数: 705371
• 初期 角度割当: タイプ: MAXIMUM LIKELIHOOD
• 最終 角度割当: タイプ: MAXIMUM LIKELIHOOD