beta-amylase / beta-amylase activity / amylopectin maltohydrolase activity / polysaccharide catabolic process / identical protein binding 類似検索 - 分子機能
Glycoside hydrolase, family 14B, plant / Beta-amylase active site 2. / Glycoside hydrolase, family 14, conserved site / Beta-amylase active site 1. / Glycoside hydrolase, family 14 / Glycosyl hydrolase family 14 / Glycosidases / Glycoside hydrolase superfamily / TIM Barrel / Alpha-Beta Barrel / Alpha Beta 類似検索 - ドメイン・相同性
SHEET DETERMINATION METHOD: DSSP BELOW IS ACTUALLY AN 8-STRANDED BARREL THIS IS REPRESENTED BY A 9- ... SHEET DETERMINATION METHOD: DSSP BELOW IS ACTUALLY AN 8-STRANDED BARREL THIS IS REPRESENTED BY A 9-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS ARE IDENTICAL.
3,4-EPOXYBUTYL ALPHA-D-GLUCOPYRANOSIDE: THE LIGAND WAS A MIXTURE OF TWO DIASTEREOISOMERS EPIMERIC ...3,4-EPOXYBUTYL ALPHA-D-GLUCOPYRANOSIDE: THE LIGAND WAS A MIXTURE OF TWO DIASTEREOISOMERS EPIMERIC AT THE 3 POSITION. I.E. 3R,S-3,4-EPOXYBUTYL ALPHA-D-GLUCOPYRANOSIDE. THIS WAS SOAKED INTO CRYSTALS AT 10 MM FOR 24 HOURS. BOTH OF THE DIASTEREOISOMERS REACT WITH THE PROTEIN GIVING COVALENT LINKS TO ATOM OE2 OF GLU 184. THE RESULTANT COMPLEX IS THEREFORE A MIXTURE OF TWO DIASTEREOISOMERS. 2S-2-HYDROXY-4-ALPHA-D-GLUCOPYRANOSYLOXY BUTOXY-GAMMA-CARBONYL-GLU184 (EBG-GLU184) HAS BEEN REFINED WITH AN OCCUPANCY OF 0.6 AND 2R-2-HYDROXY-4-ALPHA-D-GLUCOPYRANOSYLOXY BUTOXY-GAMMA-CARBONYL-GLU184 (EBQ-GLU184)HAS BEEN REFINED WITH AN OCCUPANCY OF 0.4. 1,2-ETHANEDIOL: PRESENT AT 20 PERCENT IN THE CRYOPROTECTANT
配列の詳細
PROTEIN ISOLATED FROM NATURAL SOURCE BUT SEQUENCE DIFFERS FROM UNIPROTKB DATABASE ENTRY P16098 AT ...PROTEIN ISOLATED FROM NATURAL SOURCE BUT SEQUENCE DIFFERS FROM UNIPROTKB DATABASE ENTRY P16098 AT THREE POSITIONS. THESE CHANGES WERE IDENTIFIED FROM ELECTRON DENSITY MAPS AND ARE ATTRIBUTED TO NATURAL VARIATION
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実験情報
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実験
実験
手法: X線回折 / 使用した結晶の数: 1
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試料調製
結晶
マシュー密度: 1.91 Å3/Da / 溶媒含有率: 35.6 % / 解説: NONE
結晶化
温度: 291 K / 手法: 蒸気拡散法, ハンギングドロップ法 / pH: 5.5 詳細: CRYSTALS WERE GROWN AT 291 K USING THE HANGING DROP VAPOUR DIFFUSION METHOD WITH PROTEIN AT 10 MG PER ML AND A PRECIPITANT COMPRISED OF 14 PERCENT PEG 3350 IN 100 MM BIS-TRIS PROPANE BUFFER AT PH 5.5
解像度: 1.3→24.701 Å / Cor.coef. Fo:Fc: 0.98 / Cor.coef. Fo:Fc free: 0.974 / SU B: 1.316 / SU ML: 0.025 / 交差検証法: THROUGHOUT / σ(F): 0 / ESU R: 0.049 / ESU R Free: 0.046 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD / 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.
Rfactor
反射数
%反射
Selection details
Rfree
0.1578
5424
5.03 %
RANDOM
Rwork
0.1279
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obs
0.129
109295
98.075 %
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溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.4 Å / 溶媒モデル: MASK BULK SOLVENT