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Yorodumi- PDB-2mgy: Solution structure of the mitochondrial translocator protein (TSP... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2mgy | ||||||
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Title | Solution structure of the mitochondrial translocator protein (TSPO) in complex with its high-affinity ligand PK11195 | ||||||
Components | Translocator protein | ||||||
Keywords | MEMBRANE PROTEIN / translocator protein (TSPO) / protein-ligand complex / mitochondrial membrane / PK11195 / DPC micelles / peripheral benzodiazepine receptor / helical membrane channel protein | ||||||
Function / homology | Function and homology information negative regulation of corticosterone secretion / Pregnenolone biosynthesis / negative regulation of ATP metabolic process / response to vitamin B1 / contact inhibition / peripheral nervous system axon regeneration / regulation of steroid biosynthetic process / maintenance of protein location in mitochondrion / benzodiazepine receptor activity / establishment of protein localization to mitochondrion ...negative regulation of corticosterone secretion / Pregnenolone biosynthesis / negative regulation of ATP metabolic process / response to vitamin B1 / contact inhibition / peripheral nervous system axon regeneration / regulation of steroid biosynthetic process / maintenance of protein location in mitochondrion / benzodiazepine receptor activity / establishment of protein localization to mitochondrion / glial cell migration / response to acetylcholine / cellular hypotonic response / androgen binding / negative regulation of nitric oxide biosynthetic process / steroid biosynthetic process / positive regulation of calcium ion transport / negative regulation of mitophagy / positive regulation of programmed necrotic cell death / response to manganese ion / chloride transport / cellular response to zinc ion / lipid transport / negative regulation of glial cell proliferation / adrenal gland development / cholesterol binding / positive regulation of mitochondrial depolarization / response to testosterone / behavioral response to pain / negative regulation of tumor necrosis factor production / monoatomic ion transport / positive regulation of glial cell proliferation / negative regulation of protein ubiquitination / cholesterol homeostasis / response to progesterone / positive regulation of reactive oxygen species metabolic process / cellular response to lipopolysaccharide / transmembrane transporter binding / mitochondrial outer membrane / response to xenobiotic stimulus / positive regulation of apoptotic process / endoplasmic reticulum / mitochondrion / membrane / cytosol Similarity search - Function | ||||||
Biological species | Mus musculus (house mouse) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Jaremko, M. / Jaremko, L. / Giller, K. / Becker, S. / Zweckstetter, M. | ||||||
Citation | Journal: Science / Year: 2014 Title: Structure of the mitochondrial translocator protein in complex with a diagnostic ligand. Authors: Jaremko, L. / Jaremko, M. / Giller, K. / Becker, S. / Zweckstetter, M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2mgy.cif.gz | 1 MB | Display | PDBx/mmCIF format |
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PDB format | pdb2mgy.ent.gz | 875.4 KB | Display | PDB format |
PDBx/mmJSON format | 2mgy.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2mgy_validation.pdf.gz | 626.6 KB | Display | wwPDB validaton report |
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Full document | 2mgy_full_validation.pdf.gz | 1.5 MB | Display | |
Data in XML | 2mgy_validation.xml.gz | 240.9 KB | Display | |
Data in CIF | 2mgy_validation.cif.gz | 192.2 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/mg/2mgy ftp://data.pdbj.org/pub/pdb/validation_reports/mg/2mgy | HTTPS FTP |
-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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NMR ensembles |
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-Components
#1: Protein | Mass: 18798.684 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Gene: Tspo, Bzrp / Plasmid: pET15PBR / Production host: Escherichia coli (E. coli) / References: UniProt: Q99M32, UniProt: P50637*PLUS |
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#2: Chemical | ChemComp-PKA / |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample |
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Sample conditions | Ionic strength: 10 / pH: 6 / Pressure: ambient / Temperature: 315 K |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | ||||||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 105 / Conformers submitted total number: 20 |