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Yorodumi- PDB-2lig: THREE-DIMENSIONAL STRUCTURES OF THE LIGAND-BINDING DOMAIN OF THE ... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2lig | ||||||
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Title | THREE-DIMENSIONAL STRUCTURES OF THE LIGAND-BINDING DOMAIN OF THE BACTERIAL ASPARTATE RECEPTOR WITH AND WITHOUT A LIGAND | ||||||
Components | ASPARTATE RECEPTOR | ||||||
Keywords | CHEMOTAXIS | ||||||
Function / homology | Function and homology information transmembrane signaling receptor activity / chemotaxis / signal transduction / plasma membrane Similarity search - Function | ||||||
Biological species | Salmonella typhimurium (bacteria) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 2 Å | ||||||
Authors | Kim, S.-H. / Yeh, J.I. / Prive, G.G. / Milburn, M. / Scott, W. / Koshland Junior, D.E. | ||||||
Citation | Journal: Science / Year: 1991 Title: Three-dimensional structures of the ligand-binding domain of the bacterial aspartate receptor with and without a ligand. Authors: Milburn, M.V. / Prive, G.G. / Milligan, D.L. / Scott, W.G. / Yeh, J. / Jancarik, J. / Koshland Jr., D.E. / Kim, S.H. #1: Journal: J.Biol.Chem. / Year: 1993 Title: The Three-Dimensional Structure of the Ligand-Binding Domain of a Wild-Type Bacterial Chemotaxis Receptor. Structural Comparison to the Cross-Linked Mutant Forms and Conformational Changes Upon Ligand Binding Authors: Yeh, J.I. / Biemann, H.-P. / Pandit, J. / Koshland, D.E. / Kim, S.-H. #2: Journal: J.Mol.Biol. / Year: 1993 Title: Refined Structures of the Ligand-Binding Domain of the Aspartate Receptor from Salmonella Typhimurium Authors: Scott, W.G. / Milligan, D.L. / Milburn, M.V. / Prive, G.G. / Yeh, J. / Koshland Junior, D.E. / Kim, S.-H. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2lig.cif.gz | 69.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2lig.ent.gz | 55.3 KB | Display | PDB format |
PDBx/mmJSON format | 2lig.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2lig_validation.pdf.gz | 415.4 KB | Display | wwPDB validaton report |
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Full document | 2lig_full_validation.pdf.gz | 434.6 KB | Display | |
Data in XML | 2lig_validation.xml.gz | 10.7 KB | Display | |
Data in CIF | 2lig_validation.cif.gz | 15.5 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/li/2lig ftp://data.pdbj.org/pub/pdb/validation_reports/li/2lig | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Atom site foot note | 1: PHE B 180 - ASP B 181 OMEGA = 100.60 PEPTIDE BOND DEVIATES SIGNIFICANTLY FROM TRANS CONFORMATION |
-Components
#1: Protein | Mass: 18371.432 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Salmonella typhimurium (bacteria) / Production host: Escherichia coli (E. coli) / References: UniProt: P02941 #2: Chemical | #3: Chemical | ChemComp-ASP / | #4: Chemical | ChemComp-PHN / | #5: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.32 Å3/Da / Density % sol: 46.94 % | ||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | *PLUS Temperature: 21 ℃ / pH: 7.4 / Method: vapor diffusion, sitting drop | ||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Processing
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Refinement | Rfactor Rwork: 0.176 / Rfactor obs: 0.176 / Highest resolution: 2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Highest resolution: 2 Å
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Refine LS restraints |
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