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Yorodumi- EMDB-27562: Postfusion Nipah virus fusion protein in complex with Fab 1H1, lo... -
+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-27562 | |||||||||
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Title | Postfusion Nipah virus fusion protein in complex with Fab 1H1, low resolution reconstruction including the six helix bundle | |||||||||
Map data | Postfusion Nipah virus fusion protein in complex with Fab 1H1, low resolution reconstruction including the six helix bundle | |||||||||
Sample |
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Keywords | Nipah / Nipah virus / NiV / fusion / F / antibody / neutralizing / conserved epitope / neutralizing antibody / six helix bundle / VIRAL PROTEIN | |||||||||
Biological species | Nipah henipavirus | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.9 Å | |||||||||
Authors | Byrne PO / Blade EG / McLellan JS | |||||||||
Funding support | United States, 1 items
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Citation | Journal: To Be Published Title: Postfusion Nipah virus fusion protein in complex with Fab 1H1 Authors: Byrne PO / Blade EG / McLellan JS | |||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_27562.map.gz | 684 MB | EMDB map data format | |
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Header (meta data) | emd-27562-v30.xml emd-27562.xml | 13.7 KB 13.7 KB | Display Display | EMDB header |
FSC (resolution estimation) | emd_27562_fsc.xml | 20.8 KB | Display | FSC data file |
Images | emd_27562.png | 51.8 KB | ||
Masks | emd_27562_msk_1.map | 729 MB | Mask map | |
Others | emd_27562_additional_1.map.gz emd_27562_half_map_1.map.gz emd_27562_half_map_2.map.gz | 362.8 MB 676.2 MB 676.3 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-27562 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-27562 | HTTPS FTP |
-Validation report
Summary document | emd_27562_validation.pdf.gz | 1.1 MB | Display | EMDB validaton report |
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Full document | emd_27562_full_validation.pdf.gz | 1.1 MB | Display | |
Data in XML | emd_27562_validation.xml.gz | 28.3 KB | Display | |
Data in CIF | emd_27562_validation.cif.gz | 37.4 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-27562 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-27562 | HTTPS FTP |
-Related structure data
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_27562.map.gz / Format: CCP4 / Size: 729 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||
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Annotation | Postfusion Nipah virus fusion protein in complex with Fab 1H1, low resolution reconstruction including the six helix bundle | ||||||||||||||||||||
Voxel size | X=Y=Z: 0.81 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Mask #1
File | emd_27562_msk_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Additional map: Additional Map
File | emd_27562_additional_1.map | ||||||||||||
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Annotation | Additional Map | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: Half Map 1
File | emd_27562_half_map_1.map | ||||||||||||
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Annotation | Half Map 1 | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: Half Map 2
File | emd_27562_half_map_2.map | ||||||||||||
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Annotation | Half Map 2 | ||||||||||||
Projections & Slices |
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Density Histograms |
-Sample components
-Entire : Postfusion Nipah virus fusion protein in complex with Fab 1H1
Entire | Name: Postfusion Nipah virus fusion protein in complex with Fab 1H1 |
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Components |
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-Supramolecule #1: Postfusion Nipah virus fusion protein in complex with Fab 1H1
Supramolecule | Name: Postfusion Nipah virus fusion protein in complex with Fab 1H1 type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#3 |
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Source (natural) | Organism: Nipah henipavirus |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 8 |
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Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 70.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.5 µm / Nominal defocus min: 1.5 µm |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |