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- EMDB-27091: CryoEM structure of amplified alpha-synuclein fibril class A type... -
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Open data
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Basic information
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Title | CryoEM structure of amplified alpha-synuclein fibril class A type I with extended core from DLB case VII | |||||||||
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Function / homology | ![]() negative regulation of mitochondrial electron transport, NADH to ubiquinone / neutral lipid metabolic process / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | helical reconstruction / ![]() | |||||||||
![]() | Zhou Y / Sokratian A / Xu E / Viverette E / Dillard L / Yuan Y / Li JY / Matarangas A / Bouvette J / Borgnia M ...Zhou Y / Sokratian A / Xu E / Viverette E / Dillard L / Yuan Y / Li JY / Matarangas A / Bouvette J / Borgnia M / Bartesaghi A / West A | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Structural and functional landscape of alpha-synuclein fibril assemblies amplified from cerebrospinal fluid Authors: Zhou Y / Sokratian A / Xu E / Viverette E / Dillard L / Yuan Y / Li JY / Matarangas A / Bouvette J / Borgnia M / Bartesaghi A / West A | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 1.2 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 17.7 KB 17.7 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 9.1 KB | Display | ![]() |
Images | ![]() | 97.4 KB | ||
Masks | ![]() | 64 MB | ![]() | |
Filedesc metadata | ![]() | 5.6 KB | ||
Others | ![]() ![]() | 49.6 MB 49.6 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8cz2MC ![]() 8cysC ![]() 8cytC ![]() 8cyvC ![]() 8cywC ![]() 8cyxC ![]() 8cyyC ![]() 8cz0C ![]() 8cz1C ![]() 8cz3C ![]() 8cz6C C: citing same article ( M: atomic model generated by this map |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Map
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Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.08 Å | ||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Mask #1
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Density Histograms |
-Half map: #2
File | emd_27091_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #1
File | emd_27091_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
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Sample components
-Entire : Amplified alpha-synuclein fibrils from Lewy body dementia CSF
Entire | Name: Amplified alpha-synuclein fibrils from Lewy body dementia CSF |
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Components |
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-Supramolecule #1: Amplified alpha-synuclein fibrils from Lewy body dementia CSF
Supramolecule | Name: Amplified alpha-synuclein fibrils from Lewy body dementia CSF type: organelle_or_cellular_component / ID: 1 / Parent: 0 / Macromolecule list: all Details: alpha-synuclein fibril produced in presence of postmortem cerebrospinal fluid from specimens affected by synucleinopathies |
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Source (natural) | Organism: ![]() ![]() |
Molecular weight | Theoretical: 60 kDa/nm |
-Macromolecule #1: Alpha-synuclein
Macromolecule | Name: Alpha-synuclein / type: protein_or_peptide / ID: 1 / Number of copies: 12 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() ![]() |
Molecular weight | Theoretical: 14.476108 KDa |
Recombinant expression | Organism: ![]() ![]() ![]() |
Sequence | String: MDVFMKGLSK AKEGVVAAAE KTKQGVAEAA GKTKEGVLYV GSKTKEGVVH GVATVAEKTK EQVTNVGGAV VTGVTAVAQK TVEGAGSIA AATGFVKKDQ LGKNEEGAPQ EGILEDMPVD PDNEAYEMPS EEGYQDYEPE A UniProtKB: ![]() |
-Experimental details
-Structure determination
Method | ![]() |
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![]() | helical reconstruction |
Aggregation state | filament |
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Sample preparation
Concentration | 0.1 mg/mL | |||||||||||||||
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Buffer | pH: 7.4 Component:
Details: Gibco Cat # 10010072 | |||||||||||||||
Vitrification | Cryogen name: ETHANE / Chamber humidity: 90 % / Instrument: LEICA EM GP |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD![]() |
Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 60.0 e/Å2 |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
-Atomic model buiding 1
Refinement | Space: REAL |
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Output model | ![]() PDB-8cz2: |