National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
R01-GM129245
米国
National Science Foundation (NSF, United States)
NSF DBI 1920374
米国
Howard Hughes Medical Institute (HHMI)
米国
引用
ジャーナル: Nature / 年: 2022 タイトル: Architecture and self-assembly of the jumbo bacteriophage nuclear shell. 著者: Thomas G Laughlin / Amar Deep / Amy M Prichard / Christian Seitz / Yajie Gu / Eray Enustun / Sergey Suslov / Kanika Khanna / Erica A Birkholz / Emily Armbruster / J Andrew McCammon / Rommie E ...著者: Thomas G Laughlin / Amar Deep / Amy M Prichard / Christian Seitz / Yajie Gu / Eray Enustun / Sergey Suslov / Kanika Khanna / Erica A Birkholz / Emily Armbruster / J Andrew McCammon / Rommie E Amaro / Joe Pogliano / Kevin D Corbett / Elizabeth Villa / 要旨: Bacteria encode myriad defences that target the genomes of infecting bacteriophage, including restriction-modification and CRISPR-Cas systems. In response, one family of large bacteriophages uses a ...Bacteria encode myriad defences that target the genomes of infecting bacteriophage, including restriction-modification and CRISPR-Cas systems. In response, one family of large bacteriophages uses a nucleus-like compartment to protect its replicating genomes by excluding host defence factors. However, the principal composition and structure of this compartment remain unknown. Here we find that the bacteriophage nuclear shell assembles primarily from one protein, which we name chimallin (ChmA). Combining cryo-electron tomography of nuclear shells in bacteriophage-infected cells and cryo-electron microscopy of a minimal chimallin compartment in vitro, we show that chimallin self-assembles as a flexible sheet into closed micrometre-scale compartments. The architecture and assembly dynamics of the chimallin shell suggest mechanisms for its nucleation and growth, and its role as a scaffold for phage-encoded factors mediating macromolecular transport, cytoskeletal interactions, and viral maturation.
EMPIAR-10860 (タイトル: In situ cryo-electron tomography of E. coli APEC 2248 infected by Goslar Data size: 52.2 Data #1: Unaligned tilt-movie frames as unormalized LZW-TIFF [tilt series])
想定した対称性 - 点群: C1 (非対称) / アルゴリズム: FOURIER SPACE / 解像度のタイプ: BY AUTHOR / 解像度: 12.0 Å / 解像度の算出法: FSC 0.143 CUT-OFF / ソフトウェア - 名称: RELION / 使用したサブトモグラム数: 3710
抽出
トモグラム数: 19 / 使用した粒子像数: 98981 / 参照モデル: ab initio / 手法: ab initio / ソフトウェア - 名称: Warp 詳細: Reference generated from a subset of manual picked 400 ribosomes by RELION