ジャーナル: mBio / 年: 2022 タイトル: Structures of the Type IX Secretion/Gliding Motility Motor from across the Phylum . 著者: Rory Hennell James / Justin C Deme / Alicia Hunter / Ben C Berks / Susan M Lea / 要旨: Gliding motility using cell surface adhesins, and export of proteins by the type IX secretion system (T9SS) are two phylum-specific features of the Bacteroidetes. Both of these processes are ...Gliding motility using cell surface adhesins, and export of proteins by the type IX secretion system (T9SS) are two phylum-specific features of the Bacteroidetes. Both of these processes are energized by the GldLM motor complex, which transduces the proton motive force at the inner membrane into mechanical work at the outer membrane. We previously used cryo-electron microscopy to solve the structure of the GldLM motor core from Flavobacterium johnsoniae at 3.9-Å resolution (R. Hennell James, J. C. Deme, A. Kjaer, F. Alcock, et al., Nat Microbiol 6:221-233, 2021, https://dx.doi.org/10.1038/s41564-020-00823-6). Here, we present structures of homologous complexes from a range of pathogenic and environmental species at up to 3.0-Å resolution. These structures show that the architecture of the GldLM motor core is conserved across the phylum, although there are species-specific differences at the N terminus of GldL. The resolution improvements reveal a cage-like structure that ties together the membrane-proximal cytoplasmic region of GldL and influences gliding function. These findings add detail to our structural understanding of bacterial ion-driven motors that drive the T9SS and gliding motility. Many bacteria in the phylum use the type IX secretion system to secrete proteins across their outer membrane. Most of these bacteria can also glide across surfaces using adhesin proteins that are propelled across the cell surface. Both secretion and gliding motility are driven by the GldLM protein complex, which forms a nanoscale electrochemical motor. We used cryo-electron microscopy to study the structure of the GldLM protein complex from different species, including the human pathogens Porphyromonas gingivalis and Capnocytophaga canimorsus. The organization of the motor is conserved across species, but we find species-specific structural differences and resolve motor features at higher resolution. This work improves our understanding of the type IX secretion system, which is a virulence determinant in human and animal diseases.
全体 : Type IX Secretion System/gliding motility GldM periplasmic domain
全体
名称: Type IX Secretion System/gliding motility GldM periplasmic domain
要素
複合体: Type IX Secretion System/gliding motility GldM periplasmic domain
タンパク質・ペプチド: GldM
-
超分子 #1: Type IX Secretion System/gliding motility GldM periplasmic domain
超分子
名称: Type IX Secretion System/gliding motility GldM periplasmic domain タイプ: complex / ID: 1 / 親要素: 0 / 含まれる分子: all 詳細: The structure of the periplasmic domain of GldM was solved using a sample that also included GldL and was used to solve a structure of GldLM
タイプ: MAXIMUM LIKELIHOOD / ソフトウェア - 名称: RELION (ver. 3.1)
最終 角度割当
タイプ: MAXIMUM LIKELIHOOD / ソフトウェア - 名称: RELION (ver. 3.1)
FSC曲線 (解像度の算出)
-
原子モデル構築 1
詳細
A homology model based on the structure of FjoGldM (PDB 6EY4) was used as a starting model and modified to fit the EM density using Coot. Refinement was carried out using Phenix.
精密化
空間: REAL / プロトコル: AB INITIO MODEL / 温度因子: 93.02
得られたモデル
PDB-7sb2: Structure of the periplasmic domain of GldM from Capnocytophaga canimorsus