- EMDB-24700: Metazoan pre-targeting GET complex (cBUGG-in) -
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基本情報
登録情報
データベース: EMDB / ID: EMD-24700
タイトル
Metazoan pre-targeting GET complex (cBUGG-in)
マップデータ
sharpened cBUGG-in (C2)
試料
複合体: metazoan pre-targeting GET complex cBUGG-in
タンパク質・ペプチド: ATPase GET3
タンパク質・ペプチド: Golgi to ER traffic protein 4 homolog
タンパク質・ペプチド: Large proline-rich protein BAG6
タンパク質・ペプチド: Ubiquitin-like protein 4A
リガンド: ADENOSINE-5'-TRIPHOSPHATE
リガンド: MAGNESIUM ION
リガンド: ZINC ION
キーワード
ATPase / complex / membrane protein chaperone / CHAPERONE
機能・相同性
機能・相同性情報
BAT3 complex / immune response-activating cell surface receptor signaling pathway / maintenance of unfolded protein / GET complex / ubiquitin-like protein transferase activity / tail-anchored membrane protein insertion into ER membrane / positive regulation of ERAD pathway / cytoplasmic sequestering of protein / 加水分解酵素; 酸無水物に作用 / synaptonemal complex assembly ...BAT3 complex / immune response-activating cell surface receptor signaling pathway / maintenance of unfolded protein / GET complex / ubiquitin-like protein transferase activity / tail-anchored membrane protein insertion into ER membrane / positive regulation of ERAD pathway / cytoplasmic sequestering of protein / 加水分解酵素; 酸無水物に作用 / synaptonemal complex assembly / protein insertion into ER membrane / post-translational protein targeting to endoplasmic reticulum membrane / internal peptidyl-lysine acetylation / misfolded protein binding / natural killer cell activation / endoplasmic reticulum stress-induced pre-emptive quality control / Insertion of tail-anchored proteins into the endoplasmic reticulum membrane / proteasome binding / ubiquitin-specific protease binding / regulation of embryonic development / intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress / polyubiquitin modification-dependent protein binding / intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator / proteasomal protein catabolic process / ERAD pathway / negative regulation of proteasomal ubiquitin-dependent protein catabolic process / synapse assembly / Hsp70 protein binding / kidney development / molecular function activator activity / negative regulation of proteolysis / lung development / protein modification process / regulation of protein stability / brain development / ribosome binding / chromatin organization / chromosome / protein-folding chaperone binding / ubiquitin-dependent protein catabolic process / spermatogenesis / proteasome-mediated ubiquitin-dependent protein catabolic process / molecular adaptor activity / cell differentiation / protein stabilization / intracellular membrane-bounded organelle / signaling receptor binding / ubiquitin protein ligase binding / apoptotic process / nucleolus / negative regulation of apoptotic process / ATP hydrolysis activity / extracellular exosome / nucleoplasm / ATP binding / identical protein binding / membrane / nucleus / metal ion binding / cytosol / cytoplasm 類似検索 - 分子機能
Ubl4, C-terminal TUGS domain / UBL4A-like, ubiquitin-like domain / Tethering Ubl4a to BAGS domain / Golgi to ER traffic protein 4 / Large proline-rich protein BAG6 / : / Golgi to ER traffic protein 4 / BCL2-associated athanogene 6 / Bag6, BAG-similar domain / Arsenical pump ATPase, ArsA/GET3, eukaryotic ...Ubl4, C-terminal TUGS domain / UBL4A-like, ubiquitin-like domain / Tethering Ubl4a to BAGS domain / Golgi to ER traffic protein 4 / Large proline-rich protein BAG6 / : / Golgi to ER traffic protein 4 / BCL2-associated athanogene 6 / Bag6, BAG-similar domain / Arsenical pump ATPase, ArsA/GET3, eukaryotic / Arsenical pump ATPase, ArsA/GET3 / Anion-transporting ATPase-like domain / Anion-transporting ATPase / Ubiquitin domain signature. / Ubiquitin conserved site / Ubiquitin domain / Ubiquitin family / Ubiquitin homologues / Tetratricopeptide-like helical domain superfamily / Ubiquitin domain profile. / Ubiquitin-like domain / Ubiquitin-like domain superfamily / P-loop containing nucleoside triphosphate hydrolase 類似検索 - ドメイン・相同性
Ubiquitin-like protein 4A / Large proline-rich protein BAG6 / ATPase GET3 / Golgi to ER traffic protein 4 homolog 類似検索 - 構成要素
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
DP2GM137415
American Heart Association
287375208
引用
ジャーナル: Nat Struct Mol Biol / 年: 2021 タイトル: Structural insights into metazoan pretargeting GET complexes. 著者: Alexander F A Keszei / Matthew C J Yip / Ta-Chien Hsieh / Sichen Shao / 要旨: Close coordination between chaperones is essential for protein biosynthesis, including the delivery of tail-anchored (TA) proteins containing a single C-terminal transmembrane domain to the ...Close coordination between chaperones is essential for protein biosynthesis, including the delivery of tail-anchored (TA) proteins containing a single C-terminal transmembrane domain to the endoplasmic reticulum (ER) by the conserved GET pathway. For successful targeting, nascent TA proteins must be promptly chaperoned and loaded onto the cytosolic ATPase Get3 through a transfer reaction involving the chaperone SGTA and bridging factors Get4, Ubl4a and Bag6. Here, we report cryo-electron microscopy structures of metazoan pretargeting GET complexes at 3.3-3.6 Å. The structures reveal that Get3 helix 8 and the Get4 C terminus form a composite lid over the Get3 substrate-binding chamber that is opened by SGTA. Another interaction with Get4 prevents formation of Get3 helix 4, which links the substrate chamber and ATPase domain. Both interactions facilitate TA protein transfer from SGTA to Get3. Our findings show how the pretargeting complex primes Get3 for coordinated client loading and ER targeting.