- EMDB-23404: Reconsituted 207 bp Archaeasome, Class II -
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データベース: EMDB / ID: EMD-23404
タイトル
Reconsituted 207 bp Archaeasome, Class II
マップデータ
Reconstructed density of the Arc207 archaeasome complex. 7 histone dimers wrap 207 bp of DNA through a 4 dimer (Arc120) "base" and 3 dimer (Arc90) "lid" arrangement.
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
F32GM137496
米国
引用
ジャーナル: Elife / 年: 2021 タイトル: Archaeal chromatin 'slinkies' are inherently dynamic complexes with deflected DNA wrapping pathways. 著者: Samuel Bowerman / Jeff Wereszczynski / Karolin Luger / 要旨: Eukaryotes and many archaea package their DNA with histones. While the four eukaryotic histones wrap ~147 DNA base pairs into nucleosomes, archaeal histones form 'nucleosome-like' complexes that ...Eukaryotes and many archaea package their DNA with histones. While the four eukaryotic histones wrap ~147 DNA base pairs into nucleosomes, archaeal histones form 'nucleosome-like' complexes that continuously wind between 60 and 500 base pairs of DNA ('archaeasomes'), suggested by crystal contacts and analysis of cellular chromatin. Solution structures of large archaeasomes (>90 DNA base pairs) have never been directly observed. Here, we utilize molecular dynamics simulations, analytical ultracentrifugation, and cryoEM to structurally characterize the solution state of archaeasomes on longer DNA. Simulations reveal dynamics of increased accessibility without disruption of DNA-binding or tetramerization interfaces. Mg concentration influences compaction, and cryoEM densities illustrate that DNA is wrapped in consecutive substates arranged 90 out-of-plane with one another. Without ATP-dependent remodelers, archaea may leverage these inherent dynamics to balance chromatin packing and accessibility.
ダウンロード / ファイル: emd_23404.map.gz / 形式: CCP4 / 大きさ: 64 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
注釈
Reconstructed density of the Arc207 archaeasome complex. 7 histone dimers wrap 207 bp of DNA through a 4 dimer (Arc120) "base" and 3 dimer (Arc90) "lid" arrangement.
名称: Archaeasome containing 207 bp of DNA / タイプ: complex / ID: 1 / 親要素: 0 詳細: Archaeasome (archaeal histone-DNA complex) formed by 7 histone dimers wrapping 207 bp of DNA.