National Health and Medical Research Council (NHMRC, Australia)
APP1164216
オーストラリア
引用
ジャーナル: Nat Commun / 年: 2021 タイトル: A unified route for flavivirus structures uncovers essential pocket factors conserved across pathogenic viruses. 著者: Joshua M Hardy / Natalee D Newton / Naphak Modhiran / Connor A P Scott / Hariprasad Venugopal / Laura J Vet / Paul R Young / Roy A Hall / Jody Hobson-Peters / Fasséli Coulibaly / Daniel Watterson / 要旨: The epidemic emergence of relatively rare and geographically isolated flaviviruses adds to the ongoing disease burden of viruses such as dengue. Structural analysis is key to understand and combat ...The epidemic emergence of relatively rare and geographically isolated flaviviruses adds to the ongoing disease burden of viruses such as dengue. Structural analysis is key to understand and combat these pathogens. Here, we present a chimeric platform based on an insect-specific flavivirus for the safe and rapid structural analysis of pathogenic viruses. We use this approach to resolve the architecture of two neurotropic viruses and a structure of dengue virus at 2.5 Å, the highest resolution for an enveloped virion. These reconstructions allow improved modelling of the stem region of the envelope protein, revealing two lipid-like ligands within highly conserved pockets. We show that these sites are essential for viral growth and important for viral maturation. These findings define a hallmark of flavivirus virions and a potential target for broad-spectrum antivirals and vaccine design. We anticipate the chimeric platform to be widely applicable for investigating flavivirus biology.
名称: Kunjin virus / タイプ: virus / ID: 1 / 親要素: 0 / 含まれる分子: #1-#2 詳細: Virus amplified in Aedes albopictus C6/36 cells. M and E from West Nile virus (Kunjin) form a complex that assembles into anti-parallel dimers, (M-E)2, in the T=3 icosahedral particle. NCBI-ID: 11077 / 生物種: Kunjin virus / Sci species strain: NSW2011 / ウイルスタイプ: VIRION / ウイルス・単離状態: SPECIES / ウイルス・エンベロープ: Yes / ウイルス・中空状態: No
宿主
生物種: Culex annulirostris (カ)
分子量
理論値: 22 MDa
ウイルス殻
Shell ID: 1 / 直径: 470.0 Å / T番号(三角分割数): 3
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分子 #1: Envelope protein E
分子
名称: Envelope protein E / タイプ: protein_or_peptide / ID: 1 / コピー数: 3 / 光学異性体: LEVO
凍結剤: ETHANE / チャンバー内湿度: 100 % / チャンバー内温度: 277 K / 装置: FEI VITROBOT MARK IV 詳細: 0 second wait time 2 second blot time -10 blot force 1 second drain time.