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- EMDB-22701: Structure of the EPEC type III secretion injectisome EspA filament -
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Open data
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Basic information
Entry | Database: EMDB / ID: EMD-22701 | |||||||||
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Title | Structure of the EPEC type III secretion injectisome EspA filament | |||||||||
![]() | EPEC type III secretion injectisome EspA filament | |||||||||
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![]() | Transport / filament / secretion system / PROTEIN TRANSPORT | |||||||||
Function / homology | EspA-like secreted protein / EspA-like secreted protein / EspA/CesA-like / Translocon EspA![]() | |||||||||
Biological species | ![]() ![]() ![]() ![]() | |||||||||
Method | helical reconstruction / cryo EM / Resolution: 3.56 Å | |||||||||
![]() | Lyons BJE / Atkinson CE | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Cryo-EM structure of the EspA filament from enteropathogenic Escherichia coli: Revealing the mechanism of effector translocation in the T3SS. Authors: Bronwyn J E Lyons / Claire E Atkinson / Wanyin Deng / Antonio Serapio-Palacios / B Brett Finlay / Natalie C J Strynadka / ![]() Abstract: The type III secretion system (T3SS) is a virulence mechanism employed by Gram-negative pathogens. The T3SS forms a proteinaceous channel that projects a needle into the extracellular medium where it ...The type III secretion system (T3SS) is a virulence mechanism employed by Gram-negative pathogens. The T3SS forms a proteinaceous channel that projects a needle into the extracellular medium where it interacts with the host cell to deliver virulence factors. Enteropathogenic Escherichia coli (EPEC) is unique in adopting a needle extension to the T3SS-a filament formed by EspA-which is absolutely required for efficient colonization of the gut. Here, we describe the cryoelectron microscopy structure of native EspA filaments from EPEC at 3.6-Å resolution. Within the filament, positively charged residues adjacent to a hydrophobic groove line the lumen of the filament in a spiral manner, suggesting a mechanism of substrate translocation mediated via electrostatics. Using structure-guided mutagenesis, in vivo studies corroborate the role of these residues in secretion and translocation function. The high-resolution structure of the EspA filament could aid in structure-guided drug design of antivirulence therapeutics. | |||||||||
History |
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Structure visualization
Movie |
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Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 5.8 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 9.5 KB 9.5 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 8.8 KB | Display | ![]() |
Images | ![]() | 46.3 KB | ||
Filedesc metadata | ![]() | 4.7 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 422.4 KB | Display | ![]() |
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Full document | ![]() | 422 KB | Display | |
Data in XML | ![]() | 10 KB | Display | |
Data in CIF | ![]() | 13.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7k7kMC M: atomic model generated by this map C: citing same article ( |
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Similar structure data |
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Links
EMDB pages | ![]() ![]() |
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Map
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Annotation | EPEC type III secretion injectisome EspA filament | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.75 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
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Sample components
-Entire : EspA filament
Entire | Name: EspA filament |
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Components |
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-Supramolecule #1: EspA filament
Supramolecule | Name: EspA filament / type: organelle_or_cellular_component / ID: 1 / Parent: 0 / Macromolecule list: all |
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Source (natural) | Organism: ![]() ![]() |
-Macromolecule #1: Translocon EspA
Macromolecule | Name: Translocon EspA / type: protein_or_peptide / ID: 1 / Number of copies: 28 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() ![]() Strain: E2348/69 / EPEC |
Molecular weight | Theoretical: 20.482811 KDa |
Sequence | String: MDTSTTASVA SANASTSTSM AYDLGSMSKD DVIDLFNKLG VFQAAILMFA YMYQAQSDLS IAKFADMNEA SKESTTAQKM ANLVDAKIA DVQSSSDKNA KAQLPDEVIS YINDPRNDIT ISGIDNINAQ LGAGDLQTVK AAISAKANNL TTTVNNSQLE I QQMSNTLN ...String: MDTSTTASVA SANASTSTSM AYDLGSMSKD DVIDLFNKLG VFQAAILMFA YMYQAQSDLS IAKFADMNEA SKESTTAQKM ANLVDAKIA DVQSSSDKNA KAQLPDEVIS YINDPRNDIT ISGIDNINAQ LGAGDLQTVK AAISAKANNL TTTVNNSQLE I QQMSNTLN LLTSARSDMQ SLQYRTISGI SLGK UniProtKB: Translocon EspA |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | helical reconstruction |
Aggregation state | filament |
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Sample preparation
Buffer | pH: 7.4 |
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Vitrification | Cryogen name: ETHANE / Instrument: FEI VITROBOT MARK IV |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: FEI FALCON III (4k x 4k) / Average electron dose: 0.475 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |