SEQUENCE THERE IS ONE COMPLEX IN THE ASYMMETRIC UNIT, WHICH CONSISTS OF TWO ALPHA-BETA TUBULIN ...SEQUENCE THERE IS ONE COMPLEX IN THE ASYMMETRIC UNIT, WHICH CONSISTS OF TWO ALPHA-BETA TUBULIN HETERODIMERS (CHAINS A-B AND C-D) AND ONE STATHMIN-LIKE DOMAIN OF RB3. AS THE SEQUENCE OF BOVINE BRAIN TUBULIN IS NOT AVAILABLE, THE PIG BRAIN TUBULIN SEQUENCE WAS USED AS A REFERENCE. ONE NOTICEABLE EXCEPTION IS RESIDUE ALPHA 265 WHICH IS COMMONLY ILE BUT ALA IN PIG ALPHA TUBULIN. ALPHA-TUBULIN AND BETA-TUBULIN HAVE BEEN ALIGNED AS IN NOGALES ET AL., NATURE VOL 391, PAGES 199-203. IN THIS ALIGNMENT, RESIDUES 45-46 AND 361-368 OF ALPHA-TUBULIN ARE MISSING IN BETA-TUBULIN. THE STATHMIN-LIKE DOMAIN OF RB3 (RB3-SLD) CORRESPONDS TO STAHMIN RESIDUES 5 TO 145 WITH THE ADDITION OF AN ALANINE AT THE N-TERMINUS, WHICH IS ACETYLATED. THE NUMBERING OF RB3-SLD IS ACCORDING TO THE STATHMIN SEQUENCE.
THERE IS ONE COMPLEX IN THE ASYMMETRIC UNIT, WHICH CONSISTS OF TWO ALPHA-BETA TUBULIN HETERODIMERS (CHAINS A-B AND C-D) AND ONE STATHMIN-LIKE DOMAIN OF RB3 (CHAIN E)
解像度: 4.2→20 Å / Cor.coef. Fo:Fc: 0.956 / Cor.coef. Fo:Fc free: 0.935 / SU B: 56.857 / SU ML: 0.695 / 交差検証法: THROUGHOUT / ESU R Free: 0.873 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD 詳細: CARE SHOULD BE EXERCISED IN INTERPRETING THE CURRENT MODEL DUE TO THE LIMITED (4.2 ANGSTROMS) RESOLUTION. IN ADDITION, THE FOLLOWING WEAKLY DEFINED RESIDUES ARE MISSING IN THIS ENTRY: ...詳細: CARE SHOULD BE EXERCISED IN INTERPRETING THE CURRENT MODEL DUE TO THE LIMITED (4.2 ANGSTROMS) RESOLUTION. IN ADDITION, THE FOLLOWING WEAKLY DEFINED RESIDUES ARE MISSING IN THIS ENTRY: RESIDUES 40 TO 44 AND THE C-TERMINUS STARTING FROM RESIDUE 440 ON ALPHA TUBULIN CHAIN A, RESIDUES 277 TO 281 AND THE C-TERMINUS STARTING FROM RESIDUE 438 ON BETA TUBULIN CHAIN B, RESIDUES 39 TO 46 AND THE C-TERMINUS STARTING FROM RESIDUE 440 ON ALPHA TUBULIN CHAIN C, THE C-TERMINUS STARTING FROM RESIDUES 438 ON BETA TUBULIN CHAIN D, AND RESIDUES 4, 5, 43, 44 AND 142 TO 145 OF RB3-SLD. CA 5% OF THE SIDE CHAINS ARE POORLY DEFINED AND ARE CURRENTLY MODELLED AS ALANINES.
Rfactor
反射数
%反射
Selection details
Rfree
0.25935
1230
5 %
RANDOM
Rwork
0.20369
-
-
-
all
0.20643
-
-
-
obs
0.20643
23198
98.32 %
-
溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.4 Å / 溶媒モデル: BABINET MODEL WITH MASK
原子変位パラメータ
Biso mean: 116.49 Å2
Baniso -1
Baniso -2
Baniso -3
1-
-1.57 Å2
-0.78 Å2
0 Å2
2-
-
-1.57 Å2
0 Å2
3-
-
-
2.35 Å2
精密化ステップ
サイクル: LAST / 解像度: 4.2→20 Å
タンパク質
核酸
リガンド
溶媒
全体
原子数
13998
0
182
0
14180
拘束条件
Refine-ID
タイプ
Dev ideal
Dev ideal target
数
X-RAY DIFFRACTION
r_bond_refined_d
0.019
0.021
14508
X-RAY DIFFRACTION
r_bond_other_d
X-RAY DIFFRACTION
r_angle_refined_deg
2.091
1.96
19763
X-RAY DIFFRACTION
r_angle_other_deg
X-RAY DIFFRACTION
r_dihedral_angle_1_deg
9.523
5
1832
X-RAY DIFFRACTION
r_dihedral_angle_2_deg
X-RAY DIFFRACTION
r_dihedral_angle_3_deg
X-RAY DIFFRACTION
r_dihedral_angle_4_deg
X-RAY DIFFRACTION
r_chiral_restr
0.154
0.2
2192
X-RAY DIFFRACTION
r_gen_planes_refined
0.008
0.02
11197
X-RAY DIFFRACTION
r_gen_planes_other
X-RAY DIFFRACTION
r_nbd_refined
0.323
0.2
7768
X-RAY DIFFRACTION
r_nbd_other
X-RAY DIFFRACTION
r_nbtor_refined
X-RAY DIFFRACTION
r_nbtor_other
X-RAY DIFFRACTION
r_xyhbond_nbd_refined
0.239
0.2
646
X-RAY DIFFRACTION
r_xyhbond_nbd_other
X-RAY DIFFRACTION
r_metal_ion_refined
0.219
0.2
3
X-RAY DIFFRACTION
r_metal_ion_other
X-RAY DIFFRACTION
r_symmetry_vdw_refined
0.348
0.2
62
X-RAY DIFFRACTION
r_symmetry_vdw_other
X-RAY DIFFRACTION
r_symmetry_hbond_refined
0.212
0.2
6
X-RAY DIFFRACTION
r_symmetry_hbond_other
X-RAY DIFFRACTION
r_mcbond_it
0.069
1.5
9172
X-RAY DIFFRACTION
r_mcbond_other
X-RAY DIFFRACTION
r_mcangle_it
0.12
2
14642
X-RAY DIFFRACTION
r_scbond_it
0.156
3
5336
X-RAY DIFFRACTION
r_scangle_it
0.231
4.5
5121
X-RAY DIFFRACTION
r_rigid_bond_restr
X-RAY DIFFRACTION
r_sphericity_free
X-RAY DIFFRACTION
r_sphericity_bonded
Refine LS restraints NCS
Dom-ID: 1 / Refine-ID: X-RAY DIFFRACTION
Ens-ID
Auth asym-ID
数
タイプ
Rms dev position (Å)
Weight position
1
A
3196
tightpositional
0.07
0.05
2
B
3275
tightpositional
0.06
0.05
1
A
3196
tightthermal
0.06
0.5
2
B
3275
tightthermal
0.05
0.5
LS精密化 シェル
解像度: 4.204→4.308 Å / Total num. of bins used: 20 /