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- PDB-1lrh: Crystal structure of auxin-binding protein 1 in complex with 1-na... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1lrh | |||||||||
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Title | Crystal structure of auxin-binding protein 1 in complex with 1-naphthalene acetic acid | |||||||||
![]() | auxin-binding protein 1 | |||||||||
![]() | PROTEIN BINDING / BETA JELLYROLL / DOUBLE STRANDED PARALLEL BETA HELIX / GERMIN LIKE PROTEIN | |||||||||
Function / homology | ![]() positive regulation of DNA endoreduplication / cytokinesis by cell plate formation / auxin binding / unidimensional cell growth / auxin-activated signaling pathway / positive regulation of cell division / positive regulation of cell size / endoplasmic reticulum lumen / zinc ion binding Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | ![]() ![]() | |||||||||
![]() | Woo, E.J. / Marshall, J. / Bauly, J. / Chen, J.-G. / Venis, M. / Napier, R.M. / Pickersgill, R.W. | |||||||||
![]() | ![]() Title: Crystal structure of auxin-binding protein 1 in complex with auxin. Authors: Woo, E.J. / Marshall, J. / Bauly, J. / Chen, J.G. / Venis, M. / Napier, R.M. / Pickersgill, R.W. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 147.8 KB | Display | ![]() |
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PDB format | ![]() | 124.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.5 MB | Display | ![]() |
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Full document | ![]() | 1.6 MB | Display | |
Data in XML | ![]() | 32.7 KB | Display | |
Data in CIF | ![]() | 45.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 18411.809 Da / Num. of mol.: 4 / Mutation: D161E/E162Q Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Polysaccharide | alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)-beta-D- ...alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose Source method: isolated from a genetically manipulated source #3: Chemical | ChemComp-ZN / #4: Chemical | ChemComp-NLA / #5: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.4 Å3/Da / Density % sol: 48.81 % | |||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 5.5 Details: PEG4000, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K | |||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 291 K / pH: 7 Details: Woo, E.J., (2000) Acta Crystallogr., Sect.D, 56, 1476. | |||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.87 Å / Relative weight: 1 |
Reflection | Resolution: 1.9→30 Å / Num. all: 54476 / Num. obs: 53495 / % possible obs: 98.2 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Rmerge(I) obs: 0.038 |
Reflection shell | Resolution: 1.9→1.97 Å / Rmerge(I) obs: 0.096 / % possible all: 90 |
Reflection | *PLUS % possible obs: 94.2 % |
Reflection shell | *PLUS % possible obs: 90 % |
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Processing
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Refinement | Method to determine structure: difference fourier / Resolution: 1.9→15 Å / σ(F): 0 / Stereochemistry target values: Engh & Huber
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