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データを開く
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基本情報
登録情報 | データベース: PDB / ID: 1fkw | ||||||
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タイトル | MURINE ADENOSINE DEAMINASE (D295E) | ||||||
![]() | ADENOSINE DEAMINASE | ||||||
![]() | AMINOHYDROLASE / ZINC COFACTOR / TIM BARREL | ||||||
機能・相同性 | ![]() mature B cell apoptotic process / xanthine biosynthetic process / negative regulation of penile erection / Purine salvage / Ribavirin ADME / negative regulation of circadian sleep/wake cycle, non-REM sleep / negative regulation of mucus secretion / penile erection / purine nucleoside binding / positive regulation of germinal center formation ...mature B cell apoptotic process / xanthine biosynthetic process / negative regulation of penile erection / Purine salvage / Ribavirin ADME / negative regulation of circadian sleep/wake cycle, non-REM sleep / negative regulation of mucus secretion / penile erection / purine nucleoside binding / positive regulation of germinal center formation / negative regulation of adenosine receptor signaling pathway / histamine secretion / cytoplasmic vesicle lumen / 2'-deoxyadenosine deaminase activity / inosine biosynthetic process / amide catabolic process / adenosine deaminase / hypoxanthine biosynthetic process / germinal center B cell differentiation / adenosine catabolic process / adenosine deaminase activity / inhibition of non-skeletal tissue mineralization / hypoxanthine salvage / deoxyadenosine catabolic process / dAMP catabolic process / adenosine metabolic process / positive regulation of T cell differentiation in thymus / AMP catabolic process / dATP catabolic process / mucus secretion / negative regulation of leukocyte migration / regulation of cell-cell adhesion mediated by integrin / response to purine-containing compound / embryonic digestive tract development / allantoin metabolic process / trophectodermal cell differentiation / GMP salvage / IMP salvage / positive regulation of smooth muscle contraction / Peyer's patch development / germinal center formation / negative regulation of mature B cell apoptotic process / AMP salvage / regulation of T cell differentiation in thymus / negative regulation of thymocyte apoptotic process / anchoring junction / positive regulation of alpha-beta T cell differentiation / positive regulation of T cell differentiation / alpha-beta T cell differentiation / regulation of T cell differentiation / positive regulation of heart rate / leukocyte migration / lung alveolus development / positive regulation of T cell receptor signaling pathway / thymocyte apoptotic process / B cell proliferation / T cell differentiation / smooth muscle contraction / : / response to vitamin E / positive regulation of calcium-mediated signaling / positive regulation of B cell proliferation / T cell activation / dendrite cytoplasm / liver development / calcium-mediated signaling / lung development / placenta development / negative regulation of inflammatory response / positive regulation of T cell activation / T cell receptor signaling pathway / T cell differentiation in thymus / in utero embryonic development / lysosome / cell adhesion / response to hypoxia / external side of plasma membrane / neuronal cell body / apoptotic process / negative regulation of apoptotic process / extracellular space / zinc ion binding / cytoplasm / cytosol 類似検索 - 分子機能 | ||||||
生物種 | ![]() ![]() | ||||||
手法 | ![]() | ||||||
![]() | Wilson, D.K. / Quiocho, F.A. | ||||||
![]() | ![]() タイトル: Probing the functional role of two conserved active site aspartates in mouse adenosine deaminase. 著者: Sideraki, V. / Mohamedali, K.A. / Wilson, D.K. / Chang, Z. / Kellems, R.E. / Quiocho, F.A. / Rudolph, F.B. #1: ![]() タイトル: A Pre-Transition-State Mimic of an Enzyme: X-Ray Structure of Adenosine Deaminase with Bound 1-Deazaadenosine and Zinc-Activated Water 著者: Wilson, D.K. / Quiocho, F.A. #2: ![]() タイトル: Atomic Structure of Adenosine Deaminase Complexed with a Transition-State Analog: Understanding Catalysis and Immunodeficiency Mutations 著者: Wilson, D.K. / Rudolph, F.B. / Quiocho, F.A. | ||||||
履歴 |
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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ダウンロードとリンク
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ダウンロード
PDBx/mmCIF形式 | ![]() | 84.2 KB | 表示 | ![]() |
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PDB形式 | ![]() | 62.2 KB | 表示 | ![]() |
PDBx/mmJSON形式 | ![]() | ツリー表示 | ![]() | |
その他 | ![]() |
-検証レポート
文書・要旨 | ![]() | 448.9 KB | 表示 | ![]() |
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文書・詳細版 | ![]() | 457.5 KB | 表示 | |
XML形式データ | ![]() | 9.3 KB | 表示 | |
CIF形式データ | ![]() | 13.8 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
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リンク
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集合体
登録構造単位 | ![]()
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単位格子 |
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要素
#1: タンパク質 | 分子量: 39729.215 Da / 分子数: 1 / 変異: D295E / 由来タイプ: 組換発現 / 詳細: LIGANDED TO ZINC, PURINE RIBOSIDE / 由来: (組換発現) ![]() ![]() ![]() ![]() |
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#2: 化合物 | ChemComp-ZN / |
#3: 化合物 | ChemComp-PUR / |
#4: 水 | ChemComp-HOH / |
-実験情報
-実験
実験 | 手法: ![]() |
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試料調製
結晶 | マシュー密度: 3.55 Å3/Da / 溶媒含有率: 65.31 % | ||||||||||||||||||||||||||||||||||||
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結晶化 | *PLUS pH: 4.2 / 手法: 蒸気拡散法 | ||||||||||||||||||||||||||||||||||||
溶液の組成 | *PLUS
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-データ収集
放射光源 | 波長: 1.5418 |
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検出器 | タイプ: XUONG-HAMLIN MULTIWIRE / 検出器: AREA DETECTOR / 日付: 1993年9月6日 |
放射 | 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray |
放射波長 | 波長: 1.5418 Å / 相対比: 1 |
反射 | Num. obs: 24014 / 冗長度: 1.7 % / Rmerge(I) obs: 0.0512 |
反射 | *PLUS 最高解像度: 2.4 Å / Num. measured all: 40777 / Rmerge(I) obs: 0.051 |
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解析
ソフトウェア |
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精密化 | 解像度: 2.4→8 Å / σ(F): 1 詳細: THE "EFFECTOR" REGION (RESIDUES 40 - 67) IS INVISIBLE AND DOMAIN III IS DISORDERED AND POORLY VISIBLE IN MAPS. ASSIGNMENT OF SEQUENCE OF THE MODEL OF DOMAIN III IS VERY UNCERTAIN (RESIDUES ...詳細: THE "EFFECTOR" REGION (RESIDUES 40 - 67) IS INVISIBLE AND DOMAIN III IS DISORDERED AND POORLY VISIBLE IN MAPS. ASSIGNMENT OF SEQUENCE OF THE MODEL OF DOMAIN III IS VERY UNCERTAIN (RESIDUES 400 - 475). TEMPERATURE FACTORS HAVE NOT BEEN REFINED AND SOLVENT MOLECULES HAVE NOT BEEN INCLUDED.
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精密化ステップ | サイクル: LAST / 解像度: 2.4→8 Å
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拘束条件 |
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ソフトウェア | *PLUS 名称: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
拘束条件 | *PLUS タイプ: x_angle_deg / Dev ideal: 1.71 |