+Open data
-Basic information
Entry | Database: PDB / ID: 1ej3 | ||||||
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Title | CRYSTAL STRUCTURE OF AEQUORIN | ||||||
Components | AEQUORIN | ||||||
Keywords | OXIDOREDUCTASE / four EF-hand calcium-binding protein / protein-coelenterazine peroxide complex | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Aequorea aequorea (invertebrata) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / Resolution: 2.3 Å | ||||||
Authors | Head, J.F. / Inouye, S. / Teranishi, K. / Shimomura, O. | ||||||
Citation | Journal: Nature / Year: 2000 Title: The crystal structure of the photoprotein aequorin at 2.3 A resolution. Authors: Head, J.F. / Inouye, S. / Teranishi, K. / Shimomura, O. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1ej3.cif.gz | 89 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1ej3.ent.gz | 69 KB | Display | PDB format |
PDBx/mmJSON format | 1ej3.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ej/1ej3 ftp://data.pdbj.org/pub/pdb/validation_reports/ej/1ej3 | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 21658.182 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Aequorea aequorea (invertebrata) / Organ: LIGHT ORGAN / Plasmid: PIP-HE / Production host: Escherichia coli (E. coli) / References: UniProt: P02592 #2: Chemical | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.12 Å3/Da / Density % sol: 60.56 % | |||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 290 K / Method: vapor diffusion, hanging drop / pH: 7.2 Details: Ammonium sulfate, EDTA, BIS-TRIS, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 290K | |||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 17 ℃Details: drop consists of equal volume of protein and reservoir solutions | |||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 90 K |
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Diffraction source | Source: SYNCHROTRON / Site: NSLS / Beamline: X8C / Wavelength: 1.072 |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: May 5, 1999 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.072 Å / Relative weight: 1 |
Reflection | Resolution: 2.3→50 Å / Num. all: 23141 / Num. obs: 23141 / % possible obs: 92.5 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 10.1 % / Biso Wilson estimate: 24.8 Å2 / Rmerge(I) obs: 0.068 / Net I/σ(I): 24.7 |
Reflection shell | Resolution: 2.3→2.44 Å / Redundancy: 3.8 % / Rmerge(I) obs: 0.37 / Num. unique all: 2863 / % possible all: 69.8 |
Reflection shell | *PLUS % possible obs: 69.8 % |
-Processing
Software |
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Refinement | Resolution: 2.3→50 Å / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 2.3→50 Å
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Refine LS restraints |
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Software | *PLUS Name: CNS / Version: 0.4 / Classification: refinement | |||||||||||||||||||||||||
Refine LS restraints | *PLUS
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