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Yorodumi- PDB-1eht: THEOPHYLLINE-BINDING RNA IN COMPLEX WITH THEOPHYLLINE, NMR, 10 ST... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1eht | ||||||
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Title | THEOPHYLLINE-BINDING RNA IN COMPLEX WITH THEOPHYLLINE, NMR, 10 STRUCTURES | ||||||
Components | THEOPHYLLINE-BINDING RNA | ||||||
Keywords | RNA / RIBONUCLEIC ACID | ||||||
Function / homology | THEOPHYLLINE / RNA / RNA (> 10) Function and homology information | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Zimmermann, G.R. / Pardi, A. | ||||||
Citation | Journal: Nat.Struct.Biol. / Year: 1997 Title: Interlocking structural motifs mediate molecular discrimination by a theophylline-binding RNA. Authors: Zimmermann, G.R. / Jenison, R.D. / Wick, C.L. / Simorre, J.P. / Pardi, A. #1: Journal: Science / Year: 1994 Title: High-Resolution Molecular Discrimination by RNA Authors: Jenison, R.D. / Gill, S.C. / Pardi, A. / Polisky, B. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1eht.cif.gz | 216 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1eht.ent.gz | 176.6 KB | Display | PDB format |
PDBx/mmJSON format | 1eht.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1eht_validation.pdf.gz | 350.6 KB | Display | wwPDB validaton report |
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Full document | 1eht_full_validation.pdf.gz | 421.3 KB | Display | |
Data in XML | 1eht_validation.xml.gz | 10.2 KB | Display | |
Data in CIF | 1eht_validation.cif.gz | 15.7 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/eh/1eht ftp://data.pdbj.org/pub/pdb/validation_reports/eh/1eht | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: RNA chain | Mass: 10638.409 Da / Num. of mol.: 1 / Fragment: BINDING FRAGMENT, RESIDUES 1 - 33 / Source method: obtained synthetically Details: CONSENSUS SEQUENCE OF THE THEOPHYLLINE BINDING RNA APTAMER FLANKED BY A FOUR BASE-PAIR STEM, A THREE BASE-PAIR STEM, AND CAPPED BY A GAAA TETRALOOP |
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#2: Chemical | ChemComp-TEP / |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR |
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-Sample preparation
Sample conditions | pH: 6.8 / Temperature: 298 K |
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Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer | Type: Varian UNITYPLUS / Manufacturer: Varian / Model: UNITYPLUS / Field strength: 500 MHz |
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-Processing
Software |
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NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | ||||||||||||
NMR ensemble | Conformer selection criteria: LOWEST TOTAL ENERGY / Conformers calculated total number: 50 / Conformers submitted total number: 10 |