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データを開く
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基本情報
登録情報 | データベース: PDB / ID: 1dsy | ||||||
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タイトル | C2 DOMAIN FROM PROTEIN KINASE C (ALPHA) COMPLEXED WITH CA2+ AND PHOSPHATIDYLSERINE | ||||||
![]() | PROTEIN KINASE C, ALPHA TYPE | ||||||
![]() | TRANSFERASE / CALCIUM++ / PHOSPHOLIPID BINDING PROTEIN / CALCIUM-BINDING PROTEIN / PHOSPHATIDYLSERINE / PROTEIN KINASE C | ||||||
機能・相同性 | ![]() Acetylcholine regulates insulin secretion / EGFR Transactivation by Gastrin / HuR (ELAVL1) binds and stabilizes mRNA / Depolymerization of the Nuclear Lamina / Signaling by SCF-KIT / Regulation of KIT signaling / Syndecan interactions / Calmodulin induced events / Disinhibition of SNARE formation / SHC1 events in ERBB2 signaling ...Acetylcholine regulates insulin secretion / EGFR Transactivation by Gastrin / HuR (ELAVL1) binds and stabilizes mRNA / Depolymerization of the Nuclear Lamina / Signaling by SCF-KIT / Regulation of KIT signaling / Syndecan interactions / Calmodulin induced events / Disinhibition of SNARE formation / SHC1 events in ERBB2 signaling / Response to elevated platelet cytosolic Ca2+ / positive regulation of angiotensin-activated signaling pathway / desmosome assembly / positive regulation of dense core granule biogenesis / histone H3T6 kinase activity / cone photoreceptor outer segment / VEGFR2 mediated cell proliferation / calcium,diacylglycerol-dependent serine/threonine kinase activity / regulation of receptor-mediated endocytosis / ooplasm / RET signaling / central nervous system neuron axonogenesis / WNT5A-dependent internalization of FZD4 / RHO GTPases Activate NADPH Oxidases / protein kinase C / cellular response to carbohydrate stimulus / regulation of response to osmotic stress / negative regulation of glial cell apoptotic process / diacylglycerol-dependent serine/threonine kinase activity / regulation of platelet aggregation / regulation of muscle contraction / positive regulation of macrophage differentiation / positive regulation of lipopolysaccharide-mediated signaling pathway / alphav-beta3 integrin-PKCalpha complex / regulation of the force of heart contraction / induction of positive chemotaxis / negative regulation of glucose import / presynaptic cytosol / positive regulation of synapse assembly / negative regulation of MAPK cascade / regulation of synaptic vesicle exocytosis / muscle cell cellular homeostasis / response to corticosterone / positive regulation of cardiac muscle hypertrophy / positive regulation of exocytosis / Trafficking of GluR2-containing AMPA receptors / calyx of Held / intercalated disc / positive regulation of cell adhesion / positive regulation of blood vessel endothelial cell migration / positive regulation of bone resorption / chondrocyte differentiation / response to mechanical stimulus / regulation of peptidyl-tyrosine phosphorylation / positive regulation of endothelial cell proliferation / presynaptic modulation of chemical synaptic transmission / negative regulation of insulin receptor signaling pathway / positive regulation of endothelial cell migration / response to interleukin-1 / positive regulation of mitotic cell cycle / intrinsic apoptotic signaling pathway / neutrophil chemotaxis / post-translational protein modification / ciliary basal body / negative regulation of protein phosphorylation / stem cell differentiation / response to reactive oxygen species / mitochondrial membrane / positive regulation of smooth muscle cell proliferation / peptidyl-threonine phosphorylation / establishment of protein localization / response to organic cyclic compound / response to toxic substance / response to peptide hormone / intracellular calcium ion homeostasis / positive regulation of inflammatory response / positive regulation of angiogenesis / integrin binding / apical part of cell / response to estradiol / angiogenesis / response to ethanol / cell population proliferation / learning or memory / positive regulation of ERK1 and ERK2 cascade / negative regulation of translation / cell adhesion / protein kinase activity / intracellular signal transduction / positive regulation of cell migration / positive regulation of protein phosphorylation / negative regulation of cell population proliferation / axon / protein serine kinase activity / protein serine/threonine kinase activity / neuronal cell body / lipid binding / dendrite / perinuclear region of cytoplasm / enzyme binding 類似検索 - 分子機能 | ||||||
生物種 | ![]() ![]() | ||||||
手法 | ![]() | ||||||
![]() | Verdaguer, N. / Corbalan-Garcia, S. / Ochoa, W.F. / Fita, I. / Gomez-Fernandez, J.C. | ||||||
![]() | ![]() タイトル: Ca(2+) bridges the C2 membrane-binding domain of protein kinase Calpha directly to phosphatidylserine. 著者: Verdaguer, N. / Corbalan-Garcia, S. / Ochoa, W.F. / Fita, I. / Gomez-Fernandez, J.C. | ||||||
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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ダウンロードとリンク
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ダウンロード
PDBx/mmCIF形式 | ![]() | 43.9 KB | 表示 | ![]() |
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PDB形式 | ![]() | 30.1 KB | 表示 | ![]() |
PDBx/mmJSON形式 | ![]() | ツリー表示 | ![]() | |
その他 | ![]() |
-検証レポート
文書・要旨 | ![]() | 456.1 KB | 表示 | ![]() |
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文書・詳細版 | ![]() | 464.5 KB | 表示 | |
XML形式データ | ![]() | 6.6 KB | 表示 | |
CIF形式データ | ![]() | 8.6 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
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リンク
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集合体
登録構造単位 | ![]()
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単位格子 |
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要素
#1: タンパク質 | 分子量: 16240.451 Da / 分子数: 1 / 断片: CALCIUM/PHOSPHOLIPID BINDING DOMAIN / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() ![]() 参照: UniProt: P05696, 転移酵素; リンを含む基を移すもの; キナーゼ(アルコールにつなげるもの) | ||||||
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#2: 化合物 | #3: 化合物 | ChemComp-PO4 / | #4: 化合物 | ChemComp-PSF / | #5: 水 | ChemComp-HOH / | |
-実験情報
-実験
実験 | 手法: ![]() |
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試料調製
結晶 | マシュー密度: 2.82 Å3/Da / 溶媒含有率: 56.32 % | ||||||||||||||||||||||||||||||||||||||||||
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結晶化 | 温度: 293 K / 手法: 蒸気拡散法, ハンギングドロップ法 / pH: 6.5 詳細: PEG8K, POTASSIUM PHOSPHATE, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K | ||||||||||||||||||||||||||||||||||||||||||
結晶 | *PLUS 溶媒含有率: 50 % | ||||||||||||||||||||||||||||||||||||||||||
結晶化 | *PLUS 温度: 20 ℃ | ||||||||||||||||||||||||||||||||||||||||||
溶液の組成 | *PLUS
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-データ収集
回折 | 平均測定温度: 293 K |
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放射光源 | 由来: ![]() |
検出器 | タイプ: MARRESEARCH / 検出器: IMAGE PLATE / 日付: 1999年2月25日 |
放射 | プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray |
放射波長 | 波長: 1.5418 Å / 相対比: 1 |
反射 | 解像度: 2.6→20 Å / Num. all: 5950 / Num. obs: 5610 / % possible obs: 94.3 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / 冗長度: 2.9 % / Biso Wilson estimate: 36.9 Å2 / Rmerge(I) obs: 0.092 / Net I/σ(I): 9.4 |
反射 シェル | 解像度: 2.6→2.69 Å / 冗長度: 3.9 % / Rmerge(I) obs: 0.281 / Num. unique all: 562 / % possible all: 95.1 |
反射 | *PLUS Num. measured all: 17072 |
反射 シェル | *PLUS % possible obs: 95.1 % |
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解析
ソフトウェア |
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精密化 | 解像度: 2.6→18 Å / σ(F): 2 / 立体化学のターゲット値: Engh & Huber
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精密化ステップ | サイクル: LAST / 解像度: 2.6→18 Å
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拘束条件 |
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ソフトウェア | *PLUS 名称: 'CNS' / 分類: refinement | ||||||||||||||||||||
精密化 | *PLUS Rfactor Rfree: 0.233 / Rfactor Rwork: 0.195 | ||||||||||||||||||||
溶媒の処理 | *PLUS | ||||||||||||||||||||
原子変位パラメータ | *PLUS | ||||||||||||||||||||
拘束条件 | *PLUS
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