|Entry||Database: EMDB / ID: 1932|
|Title||Negative stain EM Map of the AAA protein CbbX, a red-type Rubisco activase from R. sphaeroides|
|Keywords||AAA+ protein / ATPase / Rubisco activase|
|Sample||R. sphaeroides CbbX 3D density map.|
|Source||Rhodobacter sphaeroides / archaea / ロドバクター・スファエロイデス|
|Map data||Negative stain EM reconstruction of the R. sphaeroides CbbX hexamer|
|Method||single particle reconstruction, at 21 Å resolution|
|Authors||Mueller-Cajar O / Stotz M / Wendler P / Hartl FU / Bracher A / Hayer-Hartl M|
|Citation||Nature, 2011, 479, 194-199|
|Validation Report||PDB-ID: 3zuh|
SummaryFull reportAbout validation report
|Date||Deposition: Jul 18, 2011 / Header (metadata) release: Aug 26, 2011 / Map release: Nov 3, 2011 / Last update: Sep 26, 2012|
Downloads & links
|File||emd_1932.map.gz (map file in CCP4 format, 8193 KB)|
|Projections & slices|
Images are generated by Spider package.
|Voxel size||X=Y=Z: 3.31 Å|
CCP4 map header:
-Entire R. sphaeroides CbbX 3D density map.
|Entire||Name: R. sphaeroides CbbX 3D density map. / Number of components: 1 / Oligomeric State: Hexamer|
|Mass||Theoretical: 206 kDa|
-Component #1: protein, Rubisco Activase
|Protein||Name: Rubisco Activase / a.k.a: CbbX / Oligomeric Details: Hexamer|
Details: The protein is bound to Ribulose-1,5-bisphosphate, ATP and ATPgammaS
Recombinant expression: Yes / Number of Copies: 6
|Mass||Theoretical: 206 kDa|
|Source||Species: Rhodobacter sphaeroides / archaea / ロドバクター・スファエロイデス|
|Source (engineered)||Expression System: Escherichia coli bl21(de3) / bacteria / image: Escherichia coli|
|External references||Gene Ontology: GO: 0005524|
|Sample solution||Specimen conc.: 0.072 mg/ml|
Buffer solution: 20 mM Tris pH 8.0, 50 mM NaCl, 5mM MgCl2, 1mM Ribulose-1,5-bisphosphate, 1mM ATP/ATPgammaS
|Support film||plain carbon grid|
|Staining||Grids with adsorbed protein stained with 2% (w/v) uranyl acetate for 40 seconds.|
|Vitrification||Instrument: NONE / Cryogen name: NONE|
-Electron microscopy imaging
|Imaging||Microscope: FEI TECNAI 12 / Date: Nov 19, 2010|
|Electron gun||Electron source: LAB6 / Accelerating voltage: 120 kV / Electron dose: 20 e/Å2 / Illumination mode: FLOOD BEAM|
|Lens||Magnification: 90600 X (nominal), 90600 X (calibrated)|
Astigmatism: objective lens astigmatism was corrected for at 110k magnification
Cs: 2 mm / Imaging mode: BRIGHT FIELD / Defocus: 260 - 1800 nm
|Specimen Holder||Holder: Eucentric / Model: SIDE ENTRY, EUCENTRIC|
|Camera||Detector: FEI EAGLE (2k x 2k)|
|Processing||Method: single particle reconstruction / Number of projections: 245 / Applied symmetry: C6 (6 fold cyclic)|
|3D reconstruction||Algorithm: angular reconstitution / Software: MRC, IMAGIC, SPIDER / CTF correction: phase flipping, each particle / Resolution: 21 Å / Resolution method: FSC 0.5|
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