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TitleStructure and function of the AAA+ protein CbbX, a red-type Rubisco activase.
Journal, issue, pagesNature, Vol. 479, Issue 7372, Page 194-199, Year 2011
Publish dateNov 2, 2011
AuthorsOliver Mueller-Cajar / Mathias Stotz / Petra Wendler / F Ulrich Hartl / Andreas Bracher / Manajit Hayer-Hartl /
PubMed AbstractRibulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) catalyses the fixation of atmospheric CO(2) in photosynthesis, but tends to form inactive complexes with its substrate ribulose 1,5- ...Ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) catalyses the fixation of atmospheric CO(2) in photosynthesis, but tends to form inactive complexes with its substrate ribulose 1,5-bisphosphate (RuBP). In plants, Rubisco is reactivated by the AAA(+) (ATPases associated with various cellular activities) protein Rubisco activase (Rca), but no such protein is known for the Rubisco of red algae. Here we identify the protein CbbX as an activase of red-type Rubisco. The 3.0-Å crystal structure of unassembled CbbX from Rhodobacter sphaeroides revealed an AAA(+) protein architecture. Electron microscopy and biochemical analysis showed that ATP and RuBP must bind to convert CbbX into functionally active, hexameric rings. The CbbX ATPase is strongly stimulated by RuBP and Rubisco. Mutational analysis suggests that CbbX functions by transiently pulling the carboxy-terminal peptide of the Rubisco large subunit into the hexamer pore, resulting in the release of the inhibitory RuBP. Understanding Rubisco activation may facilitate efforts to improve CO(2) uptake and biomass production by photosynthetic organisms.
External linksNature / PubMed:22048315
MethodsEM (single particle) / X-ray diffraction
Resolution3 - 21.0 Å
Structure data

1932

3zuh

EMDB-1932, PDB-3zuh:
Negative stain EM Map of the AAA protein CbbX, a red-type Rubisco activase from R. sphaeroides
Method: EM (single particle) / Resolution: 21.0 Å

PDB-3syk:
Crystal structure of the AAA+ protein CbbX, selenomethionine structure
Method: X-RAY DIFFRACTION / Resolution: 3.08 Å

PDB-3syl:
Crystal structure of the AAA+ protein CbbX, native structure
Method: X-RAY DIFFRACTION / Resolution: 3 Å

Chemicals

ChemComp-SO4:
SULFATE ION / Sulfate

ChemComp-HOH:
WATER / Water

ChemComp-RUB:
RIBULOSE-1,5-DIPHOSPHATE / Ribulose 1,5-bisphosphate

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM / Adenosine diphosphate

Source
  • rhodobacter sphaeroides (bacteria)
KeywordsCHAPERONE / photosynthesis / Rubisco activase / AAA+ protein / Calvin cycle / ATP BINDING PROTEIN

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