BRCA2-MAGE-D1 complex / negative regulation of mammary gland epithelial cell proliferation / presynaptic intermediate filament cytoskeleton / mitotic recombination-dependent replication fork processing / cellular response to camptothecin / chromosome organization involved in meiotic cell cycle / establishment of protein localization to telomere / telomere maintenance via telomere lengthening / positive regulation of DNA ligation / Impaired BRCA2 translocation to the nucleus ...BRCA2-MAGE-D1 complex / negative regulation of mammary gland epithelial cell proliferation / presynaptic intermediate filament cytoskeleton / mitotic recombination-dependent replication fork processing / cellular response to camptothecin / chromosome organization involved in meiotic cell cycle / establishment of protein localization to telomere / telomere maintenance via telomere lengthening / positive regulation of DNA ligation / Impaired BRCA2 translocation to the nucleus / Impaired BRCA2 binding to SEM1 (DSS1) / centrosome duplication / nuclear ubiquitin ligase complex / double-strand break repair involved in meiotic recombination / cellular response to hydroxyurea / replication-born double-strand break repair via sister chromatid exchange / lateral element / DNA recombinase assembly / telomere maintenance via recombination / histone H4 acetyltransferase activity / histone H3 acetyltransferase activity / regulation of DNA damage checkpoint / mitotic recombination / DNA strand invasion / Impaired BRCA2 binding to PALB2 / HDR through MMEJ (alt-NHEJ) / DNA strand exchange activity / gamma-tubulin binding / reciprocal meiotic recombination / single-stranded DNA helicase activity / DNA repair complex / Defective homologous recombination repair (HRR) due to BRCA1 loss of function / Defective HDR through Homologous Recombination Repair (HRR) due to PALB2 loss of BRCA1 binding function / Defective HDR through Homologous Recombination Repair (HRR) due to PALB2 loss of BRCA2/RAD51/RAD51C binding function / Homologous DNA Pairing and Strand Exchange / Resolution of D-loop Structures through Synthesis-Dependent Strand Annealing (SDSA) / response to UV-C / oocyte maturation / Resolution of D-loop Structures through Holliday Junction Intermediates / inner cell mass cell proliferation / ATP-dependent DNA damage sensor activity / DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator / HDR through Single Strand Annealing (SSA) / hematopoietic stem cell proliferation / Impaired BRCA2 binding to RAD51 / regulation of double-strand break repair via homologous recombination / nuclear chromosome / condensed chromosome / female gonad development / DNA unwinding involved in DNA replication / replication fork processing / male meiosis I / Transcriptional Regulation by E2F6 / Presynaptic phase of homologous DNA pairing and strand exchange / response to X-ray / condensed nuclear chromosome / intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator / ATP-dependent activity, acting on DNA / meiotic cell cycle / male germ cell nucleus / interstrand cross-link repair / DNA polymerase binding / positive regulation of mitotic cell cycle / regulation of cytokinesis / Meiotic recombination / secretory granule / cellular response to ionizing radiation / nucleotide-excision repair / response to gamma radiation / regulation of protein phosphorylation / double-strand break repair via homologous recombination / brain development / HDR through Homologous Recombination (HRR) / PML body / cellular senescence / double-strand break repair / double-stranded DNA binding / site of double-strand break / single-stranded DNA binding / chromosome, telomeric region / spermatogenesis / protease binding / DNA recombination / mitochondrial matrix / DNA repair / chromatin binding / centrosome / DNA damage response / positive regulation of DNA-templated transcription / chromatin / regulation of DNA-templated transcription / nucleolus / perinuclear region of cytoplasm / enzyme binding / ATP hydrolysis activity / protein-containing complex / mitochondrion / nucleoplasm / ATP binding / identical protein binding Similarity search - Function
: / BRCA2, OB2 / BRCA2, OB3 / Tower domain / Breast cancer type 2 susceptibility protein, helical domain / BRCA2 helical domain superfamily / BRCA2, oligonucleotide/oligosaccharide-binding, domain 3 / Tower / BRCA2, helical / Tower ...: / BRCA2, OB2 / BRCA2, OB3 / Tower domain / Breast cancer type 2 susceptibility protein, helical domain / BRCA2 helical domain superfamily / BRCA2, oligonucleotide/oligosaccharide-binding, domain 3 / Tower / BRCA2, helical / Tower / BRCA2 repeat / BRCA2, OB1 / Breast cancer type 2 susceptibility protein / BRCA2 repeat / BRCA2, oligonucleotide/oligosaccharide-binding, domain 1 / BRCA2 repeat profile. / DNA recombination/repair protein Rad51 / DNA recombination and repair protein, RecA-like / DNA recombination and repair protein Rad51-like, C-terminal / Rad51 / DNA recombination and repair protein RecA, monomer-monomer interface / RecA family profile 2. / DNA recombination and repair protein RecA-like, ATP-binding domain / RecA family profile 1. / DNA repair Rad51/transcription factor NusA, alpha-helical / Helix-hairpin-helix domain / ATPases associated with a variety of cellular activities / AAA+ ATPase domain / Nucleic acid-binding, OB-fold / P-loop containing nucleoside triphosphate hydrolase Similarity search - Domain/homology
Biotechnology and Biological Sciences Research Council (BBSRC)
2271086
United Kingdom
Citation
Journal: Nat Commun / Year: 2023 Title: Structural basis for stabilisation of the RAD51 nucleoprotein filament by BRCA2. Authors: Robert Appleby / Luay Joudeh / Katie Cobbett / Luca Pellegrini / Abstract: The BRCA2 tumour suppressor protein preserves genomic integrity via interactions with the DNA-strand exchange RAD51 protein in homology-directed repair. The RAD51-binding TR2 motif at the BRCA2 C- ...The BRCA2 tumour suppressor protein preserves genomic integrity via interactions with the DNA-strand exchange RAD51 protein in homology-directed repair. The RAD51-binding TR2 motif at the BRCA2 C-terminus is essential for protection and restart of stalled replication forks. Biochemical evidence shows that TR2 recognises filamentous RAD51, but existing models of TR2 binding to RAD51 lack a structural basis. Here we used cryo-electron microscopy and structure-guided mutagenesis to elucidate the mechanism of TR2 binding to nucleoprotein filaments of human RAD51. We find that TR2 binds across the protomer interface in the filament, acting as a brace for adjacent RAD51 molecules. TR2 targets an acidic-patch motif on human RAD51 that serves as a recruitment hub in fission yeast Rad51 for recombination mediators Rad52 and Rad55-Rad57. Our findings provide a structural rationale for RAD51 filament stabilisation by BRCA2 and reveal a common recruitment mechanism of recombination mediators to the RAD51 filament.
Entire : RAD51 filament formed on ssDNA and bound with a peptide correspon...
Entire
Name: RAD51 filament formed on ssDNA and bound with a peptide corresponding to the BRCA2 c-terminus
Components
Complex: RAD51 filament formed on ssDNA and bound with a peptide corresponding to the BRCA2 c-terminus
Complex: DNA repair protein RAD51 homolog 1
Protein or peptide: DNA repair protein RAD51 homolog 1
Complex: Breast cancer type 2 susceptibility protein
Protein or peptide: Breast cancer type 2 susceptibility protein
Complex: DNA (30-MER)
DNA: DNA (30-MER)
Ligand: ADENOSINE-5'-TRIPHOSPHATE
Ligand: CALCIUM ION
-
Supramolecule #1: RAD51 filament formed on ssDNA and bound with a peptide correspon...
Supramolecule
Name: RAD51 filament formed on ssDNA and bound with a peptide corresponding to the BRCA2 c-terminus type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#3
-
Supramolecule #2: DNA repair protein RAD51 homolog 1
Supramolecule
Name: DNA repair protein RAD51 homolog 1 / type: complex / ID: 2 / Parent: 1 / Macromolecule list: #1 Details: RAD51 was recombinantly purified BRCA2 c-terminus peptide was synthesised 30-mer ssDNA was synthesised
Source (natural)
Organism: Homo sapiens (human)
-
Supramolecule #3: Breast cancer type 2 susceptibility protein
Supramolecule
Name: Breast cancer type 2 susceptibility protein / type: complex / ID: 3 / Parent: 1 / Macromolecule list: #2
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