ジャーナル: J Struct Biol / 年: 2023 タイトル: Lipid nanodiscs as a template for high-resolution cryo-EM structures of peripheral membrane proteins. 著者: Kevin S Cannon / Reta D Sarsam / Tanita Tedamrongwanish / Kevin Zhang / Richard W Baker / 要旨: Peripheral membrane proteins are ubiquitous throughout cell biology and are required for a variety of cellular processes such as signal transduction, membrane trafficking, and autophagy. Transient ...Peripheral membrane proteins are ubiquitous throughout cell biology and are required for a variety of cellular processes such as signal transduction, membrane trafficking, and autophagy. Transient binding to the membrane has a profound impact on protein function, serving to induce conformational changes and alter biochemical and biophysical parameters by increasing the local concentration of factors and restricting diffusion to two dimensions. Despite the centrality of the membrane in serving as a template for cell biology, there are few reported high-resolution structures of peripheral membrane proteins bound to the membrane. We analyzed the utility of lipid nanodiscs to serve as a template for cryo-EM analysis of peripheral membrane proteins. We tested a variety of nanodiscs and we report a 3.3 Å structure of the AP2 clathrin adaptor complex bound to a 17-nm nanodisc, with sufficient resolution to visualize a bound lipid head group. Our data demonstrate that lipid nanodiscs are amenable to high-resolution structure determination of peripheral membrane proteins and provide a framework for extending this analysis to other systems.
全体 : AP2 bound to MSP1E3D1 nanodisc and Tgn38 cargo peptide
全体
名称: AP2 bound to MSP1E3D1 nanodisc and Tgn38 cargo peptide
要素
複合体: AP2 bound to MSP1E3D1 nanodisc and Tgn38 cargo peptide
複合体: AP-2 complex
タンパク質・ペプチド: AP-2 complex subunit alpha-2
タンパク質・ペプチド: AP-2 complex subunit beta
タンパク質・ペプチド: AP-2 complex subunit mu
タンパク質・ペプチド: AP-2 complex subunit sigma
複合体: Tgn38 cargo peptide
タンパク質・ペプチド: Tgn38
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超分子 #1: AP2 bound to MSP1E3D1 nanodisc and Tgn38 cargo peptide
超分子
名称: AP2 bound to MSP1E3D1 nanodisc and Tgn38 cargo peptide タイプ: complex / ID: 1 / 親要素: 0 / 含まれる分子: all 詳細: Nanodiscs were assembled with a lipid mixture containing 73 mol% DOPC, 15 mol% DOPS, 10 mol% PIP2, 2 mol% Tgn38 cargo peptide. Complex was formed by co-elution via gel filtration chromatography.
凍結剤: ETHANE-PROPANE / チャンバー内湿度: 100 % / チャンバー内温度: 277 K / 装置: FEI VITROBOT MARK IV / 詳細: Two sample applications..
詳細
Nanodiscs were assembled with a lipid mixture containing 73 mol% DOPC, 15 mol% DOPS, 10 mol% PIP2, 2 mol% Tgn38 cargo peptide. Complex was formed by co-elution via gel filtration chromatography.
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電子顕微鏡法
顕微鏡
FEI TALOS ARCTICA
撮影
フィルム・検出器のモデル: GATAN K3 BIOQUANTUM (6k x 4k) 平均電子線量: 55.0 e/Å2