+データを開く
-基本情報
登録情報 | データベース: EMDB / ID: EMD-15426 | |||||||||
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タイトル | Yeast RQC complex in state D | |||||||||
マップデータ | Yeast RQC complex in state D, half map B | |||||||||
試料 |
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キーワード | ribosome-associated quality control / NEMF / Listerin / CAT tailing / TRANSLATION | |||||||||
機能・相同性 | 機能・相同性情報 RQC complex / maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / ribosome-associated ubiquitin-dependent protein catabolic process / maturation of 5.8S rRNA / hexon binding / pre-mRNA 5'-splice site binding / cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / response to cycloheximide / SRP-dependent cotranslational protein targeting to membrane / GTP hydrolysis and joining of the 60S ribosomal subunit ...RQC complex / maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / ribosome-associated ubiquitin-dependent protein catabolic process / maturation of 5.8S rRNA / hexon binding / pre-mRNA 5'-splice site binding / cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / response to cycloheximide / SRP-dependent cotranslational protein targeting to membrane / GTP hydrolysis and joining of the 60S ribosomal subunit / ribosomal large subunit binding / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / Formation of a pool of free 40S subunits / negative regulation of mRNA splicing, via spliceosome / preribosome, large subunit precursor / Antigen processing: Ubiquitination & Proteasome degradation / L13a-mediated translational silencing of Ceruloplasmin expression / translational elongation / ribosomal large subunit export from nucleus / 90S preribosome / subtelomeric heterochromatin formation / regulation of translational fidelity / protein-RNA complex assembly / proteasomal protein catabolic process / ribosomal subunit export from nucleus / maturation of LSU-rRNA / cytosolic ribosome / translation initiation factor activity / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / rescue of stalled ribosome / assembly of large subunit precursor of preribosome / ribosomal large subunit biogenesis / cytosolic ribosome assembly / translational initiation / macroautophagy / maintenance of translational fidelity / RING-type E3 ubiquitin transferase / protein catabolic process / modification-dependent protein catabolic process / ubiquitin-protein transferase activity / protein tag activity / rRNA processing / ubiquitin protein ligase activity / ribosome biogenesis / viral capsid / chromatin organization / 5S rRNA binding / large ribosomal subunit rRNA binding / ribosomal large subunit assembly / ubiquitin-dependent protein catabolic process / cytoplasmic translation / cytosolic large ribosomal subunit / tRNA binding / chromosome, telomeric region / negative regulation of translation / rRNA binding / ribosome / protein ubiquitination / structural constituent of ribosome / translation / response to antibiotic / mRNA binding / ubiquitin protein ligase binding / host cell nucleus / nucleolus / RNA binding / zinc ion binding / nucleus / metal ion binding / cytosol / cytoplasm 類似検索 - 分子機能 | |||||||||
生物種 | Saccharomyces cerevisiae (パン酵母) | |||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 2.6 Å | |||||||||
データ登録者 | Tesina P / Buschauer R / Beckmann R | |||||||||
資金援助 | ドイツ, 1件
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引用 | ジャーナル: Mol Cell / 年: 2023 タイトル: Molecular basis of eIF5A-dependent CAT tailing in eukaryotic ribosome-associated quality control. 著者: Petr Tesina / Shuhei Ebine / Robert Buschauer / Matthias Thoms / Yoshitaka Matsuo / Toshifumi Inada / Roland Beckmann / 要旨: Ribosome-associated quality control (RQC) is a conserved process degrading potentially toxic truncated nascent peptides whose malfunction underlies neurodegeneration and proteostasis decline in aging. ...Ribosome-associated quality control (RQC) is a conserved process degrading potentially toxic truncated nascent peptides whose malfunction underlies neurodegeneration and proteostasis decline in aging. During RQC, dissociation of stalled ribosomes is followed by elongation of the nascent peptide with alanine and threonine residues, driven by Rqc2 independently of mRNA, the small ribosomal subunit and guanosine triphosphate (GTP)-hydrolyzing factors. The resulting CAT tails (carboxy-terminal tails) and ubiquitination by Ltn1 mark nascent peptides for proteasomal degradation. Here we present ten cryogenic electron microscopy (cryo-EM) structures, revealing the mechanistic basis of individual steps of the CAT tailing cycle covering initiation, decoding, peptidyl transfer, and tRNA translocation. We discovered eIF5A as a crucial eukaryotic RQC factor enabling peptidyl transfer. Moreover, we observed dynamic behavior of RQC factors and tRNAs allowing for processivity of the CAT tailing cycle without additional energy input. Together, these results elucidate key differences as well as common principles between CAT tailing and canonical translation. | |||||||||
履歴 |
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-構造の表示
添付画像 |
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-ダウンロードとリンク
-EMDBアーカイブ
マップデータ | emd_15426.map.gz | 20.8 MB | EMDBマップデータ形式 | |
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ヘッダ (付随情報) | emd-15426-v30.xml emd-15426.xml | 72.9 KB 72.9 KB | 表示 表示 | EMDBヘッダ |
FSC (解像度算出) | emd_15426_fsc.xml | 15.6 KB | 表示 | FSCデータファイル |
画像 | emd_15426.png | 62.3 KB | ||
Filedesc metadata | emd-15426.cif.gz | 15.4 KB | ||
その他 | emd_15426_half_map_1.map.gz emd_15426_half_map_2.map.gz | 322.6 MB 322.6 MB | ||
アーカイブディレクトリ | http://ftp.pdbj.org/pub/emdb/structures/EMD-15426 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-15426 | HTTPS FTP |
-検証レポート
文書・要旨 | emd_15426_validation.pdf.gz | 889.1 KB | 表示 | EMDB検証レポート |
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文書・詳細版 | emd_15426_full_validation.pdf.gz | 888.7 KB | 表示 | |
XML形式データ | emd_15426_validation.xml.gz | 24.5 KB | 表示 | |
CIF形式データ | emd_15426_validation.cif.gz | 31.8 KB | 表示 | |
アーカイブディレクトリ | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-15426 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-15426 | HTTPS FTP |
-関連構造データ
関連構造データ | 8agwMC 8aafC 8agtC 8aguC 8agvC 8agxC 8agzC M: このマップから作成された原子モデル C: 同じ文献を引用 (文献) |
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類似構造データ | 類似検索 - 機能・相同性F&H 検索 |
-リンク
EMDBのページ | EMDB (EBI/PDBe) / EMDataResource |
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「今月の分子」の関連する項目 |
-マップ
ファイル | ダウンロード / ファイル: emd_15426.map.gz / 形式: CCP4 / 大きさ: 347.6 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
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注釈 | Yeast RQC complex in state D, half map B | ||||||||||||||||||||||||||||||||||||
投影像・断面図 | 画像のコントロール
画像は Spider により作成 | ||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 1.059 Å | ||||||||||||||||||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
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-添付データ
-ハーフマップ: Yeast RQC complex in state D, half map A
ファイル | emd_15426_half_map_1.map | ||||||||||||
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注釈 | Yeast RQC complex in state D, half map A | ||||||||||||
投影像・断面図 |
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密度ヒストグラム |
-ハーフマップ: Yeast RQC complex in state D
ファイル | emd_15426_half_map_2.map | ||||||||||||
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注釈 | Yeast RQC complex in state D | ||||||||||||
投影像・断面図 |
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密度ヒストグラム |
-試料の構成要素
+全体 : Yeast RQC complex in state D
+超分子 #1: Yeast RQC complex in state D
+分子 #1: 60S ribosomal protein L15-A
+分子 #2: 60S ribosomal protein L16-A
+分子 #3: 60S ribosomal protein L17-A
+分子 #4: 60S ribosomal protein L18-A
+分子 #5: 60S ribosomal protein L19-A
+分子 #6: 60S ribosomal protein L20-A
+分子 #7: 60S ribosomal protein L21-A
+分子 #8: 60S ribosomal protein L22-A
+分子 #9: 60S ribosomal protein L23-A
+分子 #10: 60S ribosomal protein L24-A
+分子 #11: 60S ribosomal protein L25
+分子 #12: 60S ribosomal protein L26-A
+分子 #13: 60S ribosomal protein L27-A
+分子 #14: 60S ribosomal protein L28
+分子 #15: 60S ribosomal protein L29
+分子 #16: 60S ribosomal protein L30
+分子 #17: 60S ribosomal protein L31-A
+分子 #18: 60S ribosomal protein L32
+分子 #19: 60S ribosomal protein L33-A
+分子 #20: 60S ribosomal protein L34-A
+分子 #21: 60S ribosomal protein L35-A
+分子 #22: 60S ribosomal protein L36-A
+分子 #23: 60S ribosomal protein L37-A
+分子 #24: 60S ribosomal protein L38
+分子 #25: 60S ribosomal protein L39
+分子 #26: Ubiquitin-60S ribosomal protein L40
+分子 #27: 60S ribosomal protein L42-A
+分子 #28: 60S ribosomal protein L43-A
+分子 #29: 60S ribosomal protein L41-A
+分子 #33: 60S ribosomal protein L2-A
+分子 #34: 60S ribosomal protein L3
+分子 #35: 60S ribosomal protein L4-A
+分子 #36: 60S ribosomal protein L5
+分子 #37: 60S ribosomal protein L6-B
+分子 #38: 60S ribosomal protein L7-A
+分子 #39: 60S ribosomal protein L8-A
+分子 #40: 60S ribosomal protein L9-A
+分子 #41: 60S ribosomal protein L10
+分子 #42: 60S ribosomal protein L11-A
+分子 #43: 60S ribosomal protein L13-A
+分子 #44: 60S ribosomal protein L14-A
+分子 #45: RQC2 isoform 1
+分子 #46: E3 ubiquitin-protein ligase listerin
+分子 #47: Eukaryotic translation initiation factor 6
+分子 #49: 60S ribosomal protein L12-A
+分子 #50: 60S acidic ribosomal protein P0
+分子 #51: CAT-tailed nascent peptide
+分子 #52: 60S ribosomal protein L1-A
+分子 #30: 25S rRNA
+分子 #31: 5S rRNA
+分子 #32: 5.8S rRNA
+分子 #48: Ala tRNA
+分子 #53: MAGNESIUM ION
+分子 #54: ZINC ION
+分子 #55: SPERMIDINE
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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解析 | 単粒子再構成法 |
試料の集合状態 | particle |
-試料調製
緩衝液 | pH: 6.8 |
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凍結 | 凍結剤: ETHANE |
-電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
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撮影 | フィルム・検出器のモデル: GATAN K2 SUMMIT (4k x 4k) 平均電子線量: 46.0 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: FIELD EMISSION GUN |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / 最大 デフォーカス(公称値): 4.0 µm / 最小 デフォーカス(公称値): 0.4 µm |
実験機器 | モデル: Titan Krios / 画像提供: FEI Company |