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Open data
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Basic information
Entry | ![]() | |||||||||
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Title | KtrAB complex | |||||||||
![]() | KtrAB complex of KtrA8 ring with KtrB dimer on each side | |||||||||
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![]() | potassium transporter / membrane transport protein / MEMBRANE PROTEIN | |||||||||
Function / homology | ![]() potassium:chloride symporter activity / monoatomic cation transmembrane transporter activity / potassium ion transport / ATP binding / plasma membrane Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 2.82 Å | |||||||||
![]() | Vonck J / Stautz J | |||||||||
Funding support | ![]()
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![]() | ![]() Title: KtrAB complex Authors: Stautz J | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 161.6 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 20.2 KB 20.2 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 12.8 KB | Display | ![]() |
Images | ![]() | 176.9 KB | ||
Masks | ![]() | 178 MB | ![]() | |
Filedesc metadata | ![]() | 6.4 KB | ||
Others | ![]() ![]() ![]() | 165.9 MB 139.5 MB 139.5 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7zp9MC M: atomic model generated by this map C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
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Annotation | KtrAB complex of KtrA8 ring with KtrB dimer on each side | ||||||||||||||||||||
Voxel size | X=Y=Z: 0.831 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Mask #1
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Projections & Slices |
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Density Histograms |
-Additional map: Map at 2.54 Angstrom resolution after density modification in Phenix
File | emd_14851_additional_1.map | ||||||||||||
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Annotation | Map at 2.54 Angstrom resolution after density modification in Phenix | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: #2
File | emd_14851_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #1
File | emd_14851_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
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Sample components
-Entire : KtrAB complex with a second KtrB dimer attached
Entire | Name: KtrAB complex with a second KtrB dimer attached |
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Components |
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-Supramolecule #1: KtrAB complex with a second KtrB dimer attached
Supramolecule | Name: KtrAB complex with a second KtrB dimer attached / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#2 |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 390 KDa |
-Macromolecule #1: Ktr system potassium uptake protein A
Macromolecule | Name: Ktr system potassium uptake protein A / type: protein_or_peptide / ID: 1 / Number of copies: 8 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 23.83692 KDa |
Recombinant expression | Organism: ![]() ![]() |
Sequence | String: MKTGDKQFAV IGLGRFGLAV CKELQDSGSQ VLAVDINEDR VKEAAGFVSQ AIVANCTHEE TVAELKLDDY DMVMIAIGAD VNASILATL IAKEAGVKSV WVKANDRFQA RVLQKIGADH IIMPERDMGI RVARKMLDKR VLEFHPLGSG LAMTEFVVGS R LMGKTLSD ...String: MKTGDKQFAV IGLGRFGLAV CKELQDSGSQ VLAVDINEDR VKEAAGFVSQ AIVANCTHEE TVAELKLDDY DMVMIAIGAD VNASILATL IAKEAGVKSV WVKANDRFQA RVLQKIGADH IIMPERDMGI RVARKMLDKR VLEFHPLGSG LAMTEFVVGS R LMGKTLSD LALCKVEGVQ VLGYKRGPEI IKAPDMSTTL EIGDLIIVVG PQDKLANKLK SL UniProtKB: Ktr system potassium uptake protein A |
-Macromolecule #2: Ktr system potassium uptake protein B
Macromolecule | Name: Ktr system potassium uptake protein B / type: protein_or_peptide / ID: 2 / Number of copies: 4 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 49.707715 KDa |
Recombinant expression | Organism: ![]() ![]() |
Sequence | String: MTQFHQRGVF YVPDGKRDKA KGGEPRIILL SFLGVLLPSA VLLTLPVFSV SGLSITDALF TATSAISVTG LGVVDTGQHF TLAGKILLM CLMQIGGLGQ MTLSAVLLYM FGVRLSLRQQ ALAKEALGQE RQVNLRRLVK KIVTFALVAE AIGFVFLSYR W VPEMGWQT ...String: MTQFHQRGVF YVPDGKRDKA KGGEPRIILL SFLGVLLPSA VLLTLPVFSV SGLSITDALF TATSAISVTG LGVVDTGQHF TLAGKILLM CLMQIGGLGQ MTLSAVLLYM FGVRLSLRQQ ALAKEALGQE RQVNLRRLVK KIVTFALVAE AIGFVFLSYR W VPEMGWQT GMFYALFHSI SAFNNAGFAL FSDSMMSFVN DPLVSFTLAG LFIFGGLGFT VIGDVWRHWR KGFHFLHIHT KI MLIATPL LLLVGTVLFW LLERHNPNTM GSLTTGGQWL AAFFQSASAR TAGFNSVDLT QFTQPALLIM IVLMLIGAGS TST GGGIKV STFAVAFMAT WTFLRQKKHV VMFKRTVNWP TVTKSLAIIV VSGAILTTAM FLLMLTEKAS FDKVMFETIS AFAT VGLTA GLTAELSEPG KYIMIVVMII GRIGPLTLAY MLARPEPTLI KYPEDTVLTG UniProtKB: Ktr system potassium uptake protein B |
-Macromolecule #3: MAGNESIUM ION
Macromolecule | Name: MAGNESIUM ION / type: ligand / ID: 3 / Number of copies: 8 / Formula: MG |
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Molecular weight | Theoretical: 24.305 Da |
-Macromolecule #4: ADENOSINE-5'-DIPHOSPHATE
Macromolecule | Name: ADENOSINE-5'-DIPHOSPHATE / type: ligand / ID: 4 / Number of copies: 8 / Formula: ADP |
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Molecular weight | Theoretical: 427.201 Da |
Chemical component information | ![]() ChemComp-ADP: |
-Macromolecule #5: DODECYL-BETA-D-MALTOSIDE
Macromolecule | Name: DODECYL-BETA-D-MALTOSIDE / type: ligand / ID: 5 / Number of copies: 36 / Formula: LMT |
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Molecular weight | Theoretical: 510.615 Da |
Chemical component information | ![]() ChemComp-LMT: |
-Macromolecule #6: POTASSIUM ION
Macromolecule | Name: POTASSIUM ION / type: ligand / ID: 6 / Number of copies: 4 / Formula: K |
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Molecular weight | Theoretical: 39.098 Da |
-Macromolecule #7: water
Macromolecule | Name: water / type: ligand / ID: 7 / Number of copies: 43 / Formula: HOH |
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Molecular weight | Theoretical: 18.015 Da |
Chemical component information | ![]() ChemComp-HOH: |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 8 |
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Grid | Model: C-flat-1.2/1.3 / Material: COPPER / Pretreatment - Type: GLOW DISCHARGE |
Vitrification | Cryogen name: ETHANE / Instrument: FEI VITROBOT MARK IV |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Number grids imaged: 1 / Number real images: 2716 / Average electron dose: 40.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Calibrated defocus max: 2.5 µm / Calibrated defocus min: 1.0 µm / Calibrated magnification: 60168 / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 2.3000000000000003 µm / Nominal defocus min: 1.2 µm / Nominal magnification: 105000 |
Sample stage | Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
-Atomic model buiding 1
Details | The model was refined by phenix.real-space-refine |
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Refinement | Space: REAL / Protocol: OTHER |
Output model | ![]() PDB-7zp9: |