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データを開く
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基本情報
登録情報 | ![]() | |||||||||||||||
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タイトル | Specific features and methylation sites of a plant 80S ribosome | |||||||||||||||
![]() | 60S, 40S body and 40S head combined map. | |||||||||||||||
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![]() | Solanum lycopersicum / cytosolic ribosome / 80S / plant / rRNA / RIBOSOME | |||||||||||||||
機能・相同性 | ![]() pectinesterase / pectinesterase activity / : / cell wall modification / pectin catabolic process / endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / MAP kinase activity / protein-RNA complex assembly / translation regulator activity / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) ...pectinesterase / pectinesterase activity / : / cell wall modification / pectin catabolic process / endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / MAP kinase activity / protein-RNA complex assembly / translation regulator activity / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / rescue of stalled ribosome / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / 90S preribosome / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of LSU-rRNA / ribosomal large subunit biogenesis / chloroplast / maturation of SSU-rRNA / small-subunit processome / protein kinase C binding / modification-dependent protein catabolic process / rRNA processing / protein tag activity / ribosome biogenesis / ribosome binding / ribosomal small subunit biogenesis / ribosomal small subunit assembly / small ribosomal subunit / small ribosomal subunit rRNA binding / 5S rRNA binding / large ribosomal subunit rRNA binding / cytosolic small ribosomal subunit / cytosolic large ribosomal subunit / cytoplasmic translation / rRNA binding / negative regulation of translation / ribosome / protein ubiquitination / intracellular signal transduction / structural constituent of ribosome / ribonucleoprotein complex / translation / positive regulation of protein phosphorylation / mRNA binding / ubiquitin protein ligase binding / nucleolus / RNA binding / zinc ion binding / ATP binding / nucleus / metal ion binding / cytoplasm / cytosol 類似検索 - 分子機能 | |||||||||||||||
生物種 | ![]() ![]() | |||||||||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 2.38 Å | |||||||||||||||
![]() | Cottilli P / Itoh Y / Amunts A | |||||||||||||||
資金援助 | European Union, 4件
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![]() | ![]() タイトル: Cryo-EM structure and rRNA modification sites of a plant ribosome. 著者: Patrick Cottilli / Yuzuru Itoh / Yuko Nobe / Anton S Petrov / Purificación Lisón / Masato Taoka / Alexey Amunts / ![]() ![]() ![]() ![]() 要旨: Protein synthesis in crop plants contributes to the balance of food and fuel on our planet, which influences human metabolic activity and lifespan. Protein synthesis can be regulated with respect to ...Protein synthesis in crop plants contributes to the balance of food and fuel on our planet, which influences human metabolic activity and lifespan. Protein synthesis can be regulated with respect to changing environmental cues via the deposition of chemical modifications into rRNA. Here, we present the structure of a plant ribosome from tomato and a quantitative mass spectrometry analysis of its rRNAs. The study reveals fine features of the ribosomal proteins and 71 plant-specific rRNA modifications, and it re-annotates 30 rRNA residues in the available sequence. At the protein level, isoAsp is found in position 137 of uS11, and a zinc finger previously believed to be universal is missing from eL34, suggesting a lower effect of zinc deficiency on protein synthesis in plants. At the rRNA level, the plant ribosome differs markedly from its human counterpart with respect to the spatial distribution of modifications. Thus, it represents an additional layer of gene expression regulation, highlighting the molecular signature of a plant ribosome. The results provide a reference model of a plant ribosome for structural studies and an accurate marker for molecular ecology. | |||||||||||||||
履歴 |
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構造の表示
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ダウンロードとリンク
-EMDBアーカイブ
マップデータ | ![]() | 75 MB | ![]() | |
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ヘッダ (付随情報) | ![]() ![]() | 97.9 KB 97.9 KB | 表示 表示 | ![]() |
画像 | ![]() | 52.6 KB | ||
Filedesc metadata | ![]() | 20 KB | ||
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-検証レポート
文書・要旨 | ![]() | 465.2 KB | 表示 | ![]() |
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文書・詳細版 | ![]() | 464.7 KB | 表示 | |
XML形式データ | ![]() | 8.1 KB | 表示 | |
CIF形式データ | ![]() | 9.3 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
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リンク
EMDBのページ | ![]() ![]() |
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「今月の分子」の関連する項目 |
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マップ
ファイル | ![]() | ||||||||||||||||||||
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注釈 | 60S, 40S body and 40S head combined map. | ||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 0.83 Å | ||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||
詳細 | EMDB XML:
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-添付データ
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試料の構成要素
+全体 : 80S ribosome
+超分子 #1: 80S ribosome
+分子 #1: 60S ribosomal protein L8
+分子 #2: Ribos_L4_asso_C domain-containing protein
+分子 #3: Ribosomal protein L3
+分子 #4: Ribosomal_L18_c domain-containing protein
+分子 #5: Ribosomal_L6e_N domain-containing protein
+分子 #6: Thaliana 60S ribosomal protein L7
+分子 #7: Ribosomal_L7Ae domain-containing protein
+分子 #8: 60S ribosomal protein uL6
+分子 #9: 60S ribosomal protein L10
+分子 #10: 60S ribosomal protein uL5
+分子 #11: 60S ribosomal protein L13
+分子 #12: Ribosomal_L14e domain-containing protein
+分子 #13: Ribosomal protein L15
+分子 #14: Pectinesterase
+分子 #15: 50S ribosomal protein L22, chloroplastic
+分子 #16: Ribosomal_L18e/L15P domain-containing protein
+分子 #17: Ribosomal protein L19
+分子 #18: 60S ribosomal protein L18a
+分子 #19: 60S ribosomal protein eL21
+分子 #20: 60S ribosomal protein eL22
+分子 #21: 60S ribosomal protein uL14
+分子 #22: TRASH domain-containing protein
+分子 #23: Ribosomal_L23eN domain-containing protein
+分子 #24: KOW domain-containing protein
+分子 #25: 60S ribosomal protein L27
+分子 #26: Ribosomal_L18e/L15P domain-containing protein
+分子 #27: 60S ribosomal protein L29
+分子 #28: 60S ribosomal protein eL30
+分子 #29: 60S ribosomal protein eL31
+分子 #30: 60S ribosomal protein eL32
+分子 #31: 60S ribosomal protein eL33
+分子 #32: 60S ribosomal protein eL34
+分子 #33: Similar to 60S ribosomal protein L35
+分子 #34: 60S ribosomal protein L36
+分子 #35: Ribosomal protein L37
+分子 #36: 60S ribosomal protein L38
+分子 #37: 60S ribosomal protein eL39
+分子 #38: Ubiquitin
+分子 #39: 60S ribosomal protein eL42
+分子 #40: 60S ribosomal protein eL43
+分子 #41: Ribosomal_L28e domain-containing protein
+分子 #47: KH type-2 domain-containing protein
+分子 #48: Ribosomal_S7 domain-containing protein
+分子 #49: S10_plectin domain-containing protein
+分子 #50: 40S ribosomal protein uS19
+分子 #51: 40S ribosomal protein uS9
+分子 #52: 40S ribosomal protein uS13
+分子 #53: 40S ribosomal protein eS19
+分子 #54: Ribosomal_S10 domain-containing protein
+分子 #55: 40S ribosomal protein eS28
+分子 #56: 40S ribosomal protein uS14
+分子 #57: Mitogen-activated protein kinase
+分子 #58: 40S ribosomal protein S25
+分子 #61: 60S ribosomal protein L41
+分子 #62: 40S ribosomal protein SA
+分子 #63: 40S ribosomal protein S3a
+分子 #64: 40S ribosomal protein S4
+分子 #65: 40S ribosomal protein S7
+分子 #66: 40S ribosomal protein S8
+分子 #67: Ribosomal_S17_N domain-containing protein
+分子 #68: 40S ribosomal protein S17
+分子 #69: 40S ribosomal protein S21
+分子 #70: 40S body ribosomal protein uS12
+分子 #71: 40S ribosomal protein S26
+分子 #72: S5 DRBM domain-containing protein
+分子 #73: 40S ribosomal protein S6
+分子 #74: 40S body ribosomal protein uS4
+分子 #75: 30S ribosomal protein S15, chloroplastic
+分子 #76: Ribosomal protein S14
+分子 #77: 40S ribosomal protein S24
+分子 #78: 40S ribosomal protein S27
+分子 #79: 40S ribosomal protein S30
+分子 #80: 40S ribosomal protein S15a-1
+分子 #42: tRNA
+分子 #43: 25S rRNA
+分子 #44: 5S rRNA
+分子 #45: 5.8S rRNA
+分子 #46: 18S
+分子 #59: tRNA_1
+分子 #60: mRNA
+分子 #81: POTASSIUM ION
+分子 #82: MAGNESIUM ION
+分子 #83: ZINC ION
+分子 #84: SPERMINE
+分子 #85: SPERMIDINE
+分子 #86: 1,4-DIAMINOBUTANE
+分子 #87: beta-D-glucopyranose
+分子 #88: water
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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![]() | 単粒子再構成法 |
試料の集合状態 | particle |
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試料調製
緩衝液 | pH: 7.5 |
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凍結 | 凍結剤: ETHANE |
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電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
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撮影 | フィルム・検出器のモデル: GATAN K2 SUMMIT (4k x 4k) 平均電子線量: 30.2 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: ![]() |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD 最大 デフォーカス(公称値): 2.8000000000000003 µm 最小 デフォーカス(公称値): 1.0 µm |
実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |
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画像解析
初期モデル | モデルのタイプ: EMDB MAP EMDB ID: |
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最終 再構成 | 解像度のタイプ: BY AUTHOR / 解像度: 2.38 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 使用した粒子像数: 335806 |
初期 角度割当 | タイプ: PROJECTION MATCHING / ソフトウェア - 名称: RELION (ver. 3.0) |
最終 角度割当 | タイプ: ANGULAR RECONSTITUTION / ソフトウェア - 名称: RELION (ver. 3.1) |