EMDB-13661: Late assembly intermediate of the Trypanosoma brucei mitoribosoma...

Basic information

Entry

Database: EMDB / ID: EMD-13661

• Title: Late assembly intermediate of the Trypanosoma brucei mitoribosomal small subunit
• Map data: Late assembly intermediate of T. brucei mitoribosome

Sample

• Complex: Late assembly intermediate of the Trypanosoma brucei mitoribosomal small subunit
  • RNA: x 1 types
  • Protein or peptide: x 74 types
• Ligand: x 8 types

Function / homology

Function:

photoreactive repair / modulation of formation of structure involved in a symbiotic process / organellar small ribosomal subunit / 3-hydroxyisobutyryl-CoA hydrolase / 3-hydroxyisobutyryl-CoA hydrolase activity / mitochondrial mRNA editing complex / mitochondrial RNA processing / deoxyribodipyrimidine photo-lyase / kinetoplast / deoxyribodipyrimidine photo-lyase activity / thiosulfate sulfurtransferase activity / regulation of protein kinase A signaling / [acyl-carrier-protein] S-malonyltransferase / [acyl-carrier-protein] S-malonyltransferase activity / quorum sensing / translation factor activity, RNA binding / enoyl-CoA hydratase activity / ciliary plasm / mRNA stabilization / mitochondrial small ribosomal subunit / fatty acid beta-oxidation / superoxide dismutase / protein kinase A regulatory subunit binding / superoxide dismutase activity / RNA processing / translation initiation factor activity / FAD binding / mitochondrion organization / translational initiation / fatty acid biosynthetic process / transferase activity / ribosome / structural constituent of ribosome / translation / DNA repair / GTPase activity / mRNA binding / GTP binding / mitochondrion / DNA binding / RNA binding / nucleoplasm / nucleus / metal ion binding / cytosol / cytoplasm

Domain/homology:

Nucleoid-associated protein YbaB-like domain superfamily / : / Protein kinase A anchor protein, nuclear localisation signal domain / AKAP7 2'5' RNA ligase-like domain / Probable Zinc-ribbon domain / Probable Zinc-ribbon domain / : / Enoyl-CoA hydratase/isomerase, HIBYL-CoA-H type / Enoyl-CoA hydratase/isomerase domain / Enoyl-CoA hydratase/isomerase / : / Protein Fyv4 / IGR protein motif / IGR / Translation initiation factor IF-2, domain II / Initiation factor 2 signature. / Translation initiation factor IF-2, bacterial-like / Cyclic phosphodiesterase / Cryptochrome/DNA photolyase class 1 / Cryptochrome/DNA photolyase, FAD-binding domain / FAD binding domain of DNA photolyase / : / Elongation factor G domain 2 / Translation initiation factor IF- 2 / Translation initiation factor IF-2, domain 3 superfamily / DNA photolyase, N-terminal / Cryptochrome/photolyase, N-terminal domain superfamily / DNA photolyase / Photolyase/cryptochrome alpha/beta domain profile. / Cryptochrome/DNA photolyase, FAD-binding domain-like superfamily / Pentatricopeptide (PPR) repeat profile. / Pentatricopeptide repeat / Rhodanese-like domain / LysM domain / Rhodanese domain profile. / Rhodanese-like domain superfamily / LysM domain / Rhodanese-like domain / Manganese/iron superoxide dismutase, C-terminal / Manganese/iron superoxide dismutase, C-terminal domain superfamily / Manganese/iron superoxide dismutase, N-terminal domain superfamily / Iron/manganese superoxide dismutases, C-terminal domain / Acyl transferase / Acyl transferase domain / Acyl transferase domain in polyketide synthase (PKS) enzymes. / Acyl transferase domain superfamily / Acyl transferase/acyl hydrolase/lysophospholipase / ClpP/crotonase-like domain superfamily / Translational (tr)-type GTP-binding domain / Elongation factor Tu GTP binding domain / Translational (tr)-type guanine nucleotide-binding (G) domain profile. / Rossmann-like alpha/beta/alpha sandwich fold / Translation elongation factor EF1B/ribosomal protein S6 / Ribosomal protein S18 superfamily / Ribosomal protein S5, C-terminal / Ribosomal protein S5, C-terminal domain / Ubiquitin family / Ribosomal protein S8 / Ribosomal protein S8 superfamily / Ribosomal protein S8 / Small GTP-binding protein domain / Tetratricopeptide-like helical domain superfamily / Ribosomal protein S9 / Ribosomal protein S9/S16 / Ubiquitin domain profile. / Ribosomal protein S17/S11 / Ribosomal protein S17 / Ubiquitin-like domain / Ribosomal protein S11 superfamily / S15/NS1, RNA-binding / Translation protein, beta-barrel domain superfamily / Ribosomal protein S5 domain 2-type fold, subgroup / Ubiquitin-like domain superfamily / Ribosomal protein S5 domain 2-type fold / Nucleic acid-binding, OB-fold / P-loop containing nucleoside triphosphate hydrolase

Component:

A-kinase anchor protein 7-like phosphoesterase domain-containing protein / DNA photolyase, putative / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / 3-hydroxyisobutyryl-CoA hydrolase / Uncharacterized protein / Uncharacterized protein / Mitochondrial SSU ribosomal protein / Mitochondrial SSU ribosomal protein / Uncharacterized protein / Probable Zinc-ribbon domain-containing protein / 30S ribosomal protein S8, putative / Uncharacterized protein / Small ribosomal subunit protein mS41 / Uncharacterized protein / Uncharacterized protein / Mitochondrial ribosomal protein S18 / Small ribosomal subunit protein uS5 C-terminal domain-containing protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Trans-sialidase / [acyl-carrier-protein] S-malonyltransferase / Uncharacterized protein / 30S Ribosomal protein S17, putative / Uncharacterized protein / Target SNARE / Uncharacterized protein / Opioid growth factor receptor (OGFr) conserved domain-containing protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Pentacotripeptide-repeat region of PRORP domain-containing protein / Uncharacterized protein / Uncharacterized protein / Ribosomal protein S9 / Translation initiation factor IF-2, mitochondrial / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Trichohyalin / Uncharacterized protein / LysM domain-containing protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Pentacotripeptide-repeat region of PRORP domain-containing protein / Ubiquitin-like domain-containing protein / Uncharacterized protein / Manganese/iron superoxide dismutase C-terminal domain-containing protein / Superoxide dismutase, putative / Rhodanese domain-containing protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein

• Biological species: Trypanosoma brucei brucei (eukaryote)
• Method: single particle reconstruction / cryo EM / Resolution: 3.7 Å
• Authors: Lenarcic T / Leibundgut M / Saurer M / Ramrath DJF / Fluegel T / Boehringer D / Ban N

Funding support

Switzerland, 3 items

Organization	Grant number	Country
Swiss National Science Foundation	31003A_182341	Switzerland
European Molecular Biology Organization (EMBO)	1074-2019	Switzerland
Swiss National Science Foundation	182880	Switzerland

• Citation: Journal: Proc Natl Acad Sci U S A / Year: 2022; Title: Mitoribosomal small subunit maturation involves formation of initiation-like complexes.; Authors: Tea Lenarčič / Moritz Niemann / David J F Ramrath / Salvatore Calderaro / Timo Flügel / Martin Saurer / Marc Leibundgut / Daniel Boehringer / Céline Prange / Elke K Horn / André Schneider / Nenad Ban / ; Abstract: Mitochondrial ribosomes (mitoribosomes) play a central role in synthesizing mitochondrial inner membrane proteins responsible for oxidative phosphorylation. Although mitoribosomes from different organisms exhibit considerable structural variations, recent insights into mitoribosome assembly suggest that mitoribosome maturation follows common principles and involves a number of conserved assembly factors. To investigate the steps involved in the assembly of the mitoribosomal small subunit (mt-SSU) we determined the cryoelectron microscopy structures of middle and late assembly intermediates of the mitochondrial small subunit (mt-SSU) at 3.6- and 3.7-Å resolution, respectively. We identified five additional assembly factors that together with the mitochondrial initiation factor 2 (mt-IF-2) specifically interact with functionally important regions of the rRNA, including the decoding center, thereby preventing premature mRNA or large subunit binding. Structural comparison of assembly intermediates with mature mt-SSU combined with RNAi experiments suggests a noncanonical role of mt-IF-2 and a stepwise assembly process, where modular exchange of ribosomal proteins and assembly factors together with mt-IF-2 ensure proper 9S rRNA folding and protein maturation during the final steps of assembly.

History

Deposition	Sep 29, 2021	-
Header (metadata) release	May 4, 2022	-
Map release	May 4, 2022	-
Update	May 4, 2022	-
Current status	May 4, 2022	Processing site: PDBe / Status: Released

Map

• File: Download / File: emd_13661.map.gz / Format: CCP4 / Size: 178 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Annotation: Late assembly intermediate of T. brucei mitoribosome
• Voxel size: X=Y=Z: 1.39 Å

Density

Contour Level	By AUTHOR: 0.04
Minimum - Maximum	-0.107285425 - 0.22180325
Average (Standard dev.)	0.00089199014 (±0.008510966)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 360 360 360 Spacing 360 360 360
Cell	A=B=C: 500.4 Å α=β=γ: 90.0 °

Supplemental data

Sample components

Entire : Late assembly intermediate of the Trypanosoma brucei mitoribosoma...

• Entire: Name: Late assembly intermediate of the Trypanosoma brucei mitoribosomal small subunit

Components

• Complex: Late assembly intermediate of the Trypanosoma brucei mitoribosomal small subunit
  • RNA: 9S rRNA
  • Protein or peptide: uS3m
  • Protein or peptide: Ribosomal_S5_C domain-containing protein
  • Protein or peptide: bS6m
  • Protein or peptide: 30S ribosomal protein S8, putative
  • Protein or peptide: uS9m
  • Protein or peptide: LysM domain-containing protein
  • Protein or peptide: uS11m
  • Protein or peptide: uS12m
  • Protein or peptide: uS14m
  • Protein or peptide: uS15m
  • Protein or peptide: bS16m
  • Protein or peptide: 30S Ribosomal protein S17, putative
  • Protein or peptide: bS18m
  • Protein or peptide: uS19m
  • Protein or peptide: bS21m
  • Protein or peptide: mS22
  • Protein or peptide: mS23
  • Protein or peptide: mS26
  • Protein or peptide: mS29
  • Protein or peptide: mS33
  • Protein or peptide: mS34
  • Protein or peptide: mS35
  • Protein or peptide: mS37
  • Protein or peptide: mS38
  • Protein or peptide: Protein FYV4, mitochondrial
  • Protein or peptide: Superoxide dismutase, putative
  • Protein or peptide: Sod_Fe_C domain-containing protein
  • Protein or peptide: ECH_2 domain-containing protein
  • Protein or peptide: mS48
  • Protein or peptide: mS49
  • Protein or peptide: mS50
  • Protein or peptide: mS51
  • Protein or peptide: mS52
  • Protein or peptide: mS53
  • Protein or peptide: mS54
  • Protein or peptide: mS55
  • Protein or peptide: mS56
  • Protein or peptide: mS57
  • Protein or peptide: mS58
  • Protein or peptide: mS59
  • Protein or peptide: mS60
  • Protein or peptide: mS61
  • Protein or peptide: mS62
  • Protein or peptide: mS63
  • Protein or peptide: AKAP7_NLS domain-containing protein
  • Protein or peptide: mS65
  • Protein or peptide: mS66
  • Protein or peptide: Rhodanese domain-containing protein
  • Protein or peptide: Ubiquitin-like domain-containing protein
  • Protein or peptide: mS69
  • Protein or peptide: mS70
  • Protein or peptide: mS71
  • Protein or peptide: mS72
  • Protein or peptide: mS73
  • Protein or peptide: mS74
  • Protein or peptide: mt-SAF3
  • Protein or peptide: mt-SAF6
  • Protein or peptide: mt-SAF7
  • Protein or peptide: mt-SAF9
  • Protein or peptide: mt-SAF22
  • Protein or peptide: DNA photolyase, putative
  • Protein or peptide: Acyl transferase-like protein
  • Protein or peptide: mt-SAF37
  • Protein or peptide: mt-SAF38
  • Protein or peptide: Translation initiation factor IF-2, putative
  • Protein or peptide: mt-SAF39
  • Protein or peptide: Unk
  • Protein or peptide: Unk7
  • Protein or peptide: UnkE
  • Protein or peptide: UnkF
  • Protein or peptide: UnkG
  • Protein or peptide: UnkI
  • Protein or peptide: UnkK
  • Protein or peptide: UnkL
• Ligand: MAGNESIUM ION
• Ligand: ADENOSINE-5'-TRIPHOSPHATE
• Ligand: ZINC ION
• Ligand: URIDINE 5'-TRIPHOSPHATE
• Ligand: FLAVIN-ADENINE DINUCLEOTIDE
• Ligand: GUANOSINE-5'-DIPHOSPHATE
• Ligand: PHOSPHATE ION
• Ligand: water

Supramolecule #1: Late assembly intermediate of the Trypanosoma brucei mitoribosoma...

• Supramolecule: Name: Late assembly intermediate of the Trypanosoma brucei mitoribosomal small subunit; type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#75
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)

Macromolecule #1: 9S rRNA

• Macromolecule: Name: 9S rRNA / type: rna / ID: 1 / Number of copies: 1
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Tb427.10
• Molecular weight: Theoretical: 198.441406 KDa

Sequence

String:

UAAAUUAUGG UCAAUUGUUA GUAUUCAUAU UAAUUUUUUU AAAUGUUUUA UCAUUUUAUA AAGGUUUAUU UUUGAAAGAU UUUUUGUAU AAAAUUUUAG GAAUAGUUAA UAAUAAUUUA UAAUUUUGAU UAGAUUGUUU UGUUAAUGCU AUUAGAUGGG U GUGGAAAA AUAAAAAAAA UAAUUAAUAU AUAUCAAUAA UAAAUUAAAU UAAUCUAUUA GUCAGAAAUG GAUGCCAGCC GU UGCGGUA AUUUCUAUGC UUUUAAAUAU UAUACAAUUA UCAUAUUAAA UUGUUAAGUG CUGAUUUAAC CAAUAAAAAU AUA AAUAAU UUUUAUUUGU UUUUAAACAC CAUUAGGUAU AUGCAAAUAU AAAAUUAUAG UAAUUAUAAA UUAUAUUAUA UUAU AUUUA UUCAUAUAAU UAAUAGGAUA AUAUUUGUAG UUUUUGAUAC CAUGAUAAGG AUUAUAAAUU GAAAGUGUUA AUAUC AUAA UCAAAAUUUA UUAUUUAUAU UAAAUAUGUA UGUGUAGAUA AAAUAAGAAA UUAAAAAGGU AUUGUUGCCC ACCAAU UUU UAUAAUAAAA AUAACGUGCA GUAAUUAAUA UAUUUAUAAA AAUAUAUUUU UUUUUUUU

Macromolecule #2: uS3m

• Macromolecule: Name: uS3m / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Tb427.10
• Molecular weight: Theoretical: 9.07094 KDa

Sequence

String:

MFLIHFVHYK TILQKYTFKF KHIFLSIDKY NSLFFNISGI LIWLNIIHIN IILIKYSFFI LINNFEYLII LIST

Macromolecule #3: Ribosomal_S5_C domain-containing protein

• Macromolecule: Name: Ribosomal_S5_C domain-containing protein / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Tb427.10
• Molecular weight: Theoretical: 50.147355 KDa

Sequence

String:

MFRLTAASQK NLNSWYTKGT MRGGVPRIYY AWMRPGSFTR RRFEKMRNPF VDLETGTSLY FRDTRDSAEA IAHAADSKGI KGMDNAIDL YNEYRIVPDL YPEGFQWKHK LNTEYNQWRS NTWLTPDLIP KEHRGRFLCN FQLNIVAYDM RVVKFSPKDH R QWIYCVLY VGSGKGIAGW GRAVAPSTQE AKKEAIREAF SNIIAVDLEQ EGPMYPVRVN ADGVRVLLYP ARRIVANFRV AD ILCAFGF QHAGCRINLK ATNNPKSPTH TVEGVFEAVK ALRSVSEIAA SRGKVPHSLI YNIYPYLEEI RRRKGMMAMH PPG KDGLLM PDRVVDNRLP DHLKRGYYDD VYWKDFFAGS DEHLNEPRMG LRGDEMRRRL EEAQTSPAPT TAKDTRRRTL EDVL KRLGK TTRDLGSIPI VNPRVDVGLP THIKRNYQLH

Macromolecule #4: bS6m

• Macromolecule: Name: bS6m / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Tb427.10
• Molecular weight: Theoretical: 18.861543 KDa

Sequence

String:

MVFYSFVLVM KPRQRRFTSQ ALREIGVAVY SNGGLIRSIT NEGIMRPYSR FRDADNTPLT YARYIILQLD MGEEEMGKVD KIIREHQDV LMALKLNNLE RPVGIRSGNK ELQAAYFPLD TFTRLEEEIN WSPQTSADIY TQLEMNWKEF SRTRWSSFLR N

Macromolecule #5: 30S ribosomal protein S8, putative

• Macromolecule: Name: 30S ribosomal protein S8, putative / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Tb427.10
• Molecular weight: Theoretical: 32.766459 KDa

Sequence

String:

MLRQSLLSLA KVYGAVPPLG AAQGHRLLHG KREREGSLFA VANDVKRDER LLRQQLNALL EEERMPMQAR LQRNEAVSSH EAVPQTPLV DLPGVERRRD LPADPITRLF FQHKGDHALY YGTYDKPSVQ DDDRVQIEKR VPRRREENLY TPIYDFCHRI R EATEQRKR FVVVPSTIET RGCARVMHDH GLVAGFRDFH NDRAFAVELK YFQGDSTINV IEPCSYDGRT EFEWSPKMMR RL LNTHGIH NRLVVYICRT ADNRIIDHIH AVKENIGGRG LMMVH

Macromolecule #6: uS9m

• Macromolecule: Name: uS9m / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Tb427.10
• Molecular weight: Theoretical: 50.201016 KDa

Sequence

String:

MQKLLSPRTA RHARLFRLAG KLADSGSPGV PKSDGERLVW VNSHVRRDKD ISLSQEEERI RELMMPLEVG ENSFAANGQA THGNLFYFR EYPMYPGEYV PAEHNTLSSL RDELRLDLTA QSLKEAWMRV SGGVYFQSVD EYYASVDGLD AEQIGEVLAA L FPELNCYE AQALVQRTLE CISRPVSAAS RQLSRTITAE AVGLDNAPGH YTNFLEWMGR LTETRAFKTE HALFEFSRRK FN RDDVRVM FENYRLMSKA TLLADSADSY SHFYTVLKDF ARKVAGEDSR HQIGVRIDEA EVDPETGIAV GRGCADGEKY HFT ALLREN RDHNGIITVM GKPLSLVLDN KAWLMEMVLM PFDEANLDYR DFDAHIVSEG HAMPSIANEI AAFALRMAVA NALV KLIPL TRIPLKKSGL LSVDRRRERG QFPGYLDGKK VKRRFAKR

Macromolecule #7: LysM domain-containing protein

• Macromolecule: Name: LysM domain-containing protein / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Tb427.10
• Molecular weight: Theoretical: 94.321055 KDa

Sequence

String:

MVRRSHVVAY YWSRYRMPTQ MPKFDGPAPV AAPQSMNSTK TNEFIDPIDD KFPMSIRGPL VRPDVPEDQY VDSWYICTSM THHMGDYRP WSASAPPNAF RFRPFNEFDA KGREYVQYMR EFARFDPRKS RGNGQKGFPF RDAYLTKMNE ANQKTPPPTL E TIMDRAVR EHHQHARILS PLEVQRDVGR LEPIPSYAGK INADRSVFPF QWKTEDWYEY EVAKVRNRRF VFENTEEDGI RG SEVTYKI VLEGFWDHHV MKLAEDVCMF LKDVGRQIVE EKLVAVRRLL QGGAVDPELL AAFNCARAGP FGGLDEYDKE EVA NFLRSD LRRLEEQCLS VINRCNVPVP GATNIYDPHT SWPHVEKLEP WVRMAEFWTS SSDTSFTELE MSTAHYEFRK FFRV IICKL PFQSTEFEKR MYDIRHWLHR QTSCEFHTIY RRNVIHDSAV FPTEHDPATP TTHEHHRMFS FALDWQSAPV NRLST DTVH EGESWDAVAQ RLGCSVGELK DANAERETIE AGVVINVPVT ATRRLTSFGA TPLVLPLKTT SAKDGERIRT WEEAAA ILD CTVEELQQCN GHAALTYQKK ESEAGEFDSS VTELVAPLSC WTSTSESEFS PVERVHANDT LVAIARRLQC SEEALRA VN DGITDVSGLD FVRVPPEARR PRRLVEPQLR PQAATDALLA RTIAEEETFK LKSIPHLPQN AERFPHEYHT PTSRFPPT P SETPATQDWM AYTAKYLDKQ FTISAEPAPV YNVNKLWPMQ QIPGKVDQTP FEEDQTWLLH SIPVQQLEMH HHEKDLQDL PFINHEQFPR SLEWNAP

Macromolecule #8: uS11m

• Macromolecule: Name: uS11m / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Tb427.10
• Molecular weight: Theoretical: 37.92591 KDa

Sequence

String:

MMRKSVTFLR RGKPRPRAGM FPDKYRRVPM LLKPQQGGQQ YFNHFLIRST NDRLTQQDVD NASGQAHSFI SPQLPQMDWR NMSARSSEE SIREEMRQLA ENDVMQHQRV FNERMWYEKE EEHRMKARSC PEETSEHA(UNK)S NDGAVPPPRV LGGDYFK TR FGYSLVKNSE MTQGPVDYSQ LDMWGEMPRY TSDMVFLYLV SRRRNTYAVA YTYEGKRILN TYTAGNRGLK GGDRGFRS E GSTDNGHQVT SMYLNDLLPK LREMRASEGR PMGRGEKVEL VVRVMGFYNG RQGAVRAVQD RANEFHVRYF EDITPFPLN GPKMPRGVFK

Macromolecule #9: uS12m

• Macromolecule: Name: uS12m / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Tb427.10
• Molecular weight: Theoretical: 10.421635 KDa

Sequence

String:

LFFLRMWFLY GCCLRFVLFV LCYYMSPRLP SSGNRRVLYA VFYLYNFVWM LRCFFCCFIG LVMSLFIIEG GGFVDLPGVK YYTRIVS

Macromolecule #10: uS14m

• Macromolecule: Name: uS14m / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Tb427.10
• Molecular weight: Theoretical: 19.900014 KDa

Sequence

String:

MFCFSRGLWM RHIGQDVPKR HTHFVLESRL MYEKSFRDCW LHSVCRAISQ LDEPLSKTVV GTHQKMLQRK VTCFQYNQYG LFKTPYYRL ANVDRYHAVQ GVAGTREWVP YVNVSYWTMN KMVRGGNLLV HRVHYTGWGT DSHLKKGGWE HRWNKVLQRN V LQYSRI

Macromolecule #11: uS15m

• Macromolecule: Name: uS15m / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Tb427.10
• Molecular weight: Theoretical: 50.401191 KDa

Sequence

String:

MFVRSFCFLR KYGSKSKTPD LKTSFTKTTN FRKAEKISIT TTDGEIVKGR GKIRRRTHSA LKEAIGAMEH PAIWLWYPWR MNPEPPTPH MPQRRALKNV HGAVFNDLTP VQKKRQEQML YGVNIPETRQ MKFEEQHPLL AGALRKLEGQ PKGFPFWYRK Y PTRRHAYE YRFSIPVEML DGYNDDVKKA LSKGMMSIQE KQFAQEAMYM ERYAEHDFDT TSPAVLAVKR ALKCRVLRNH LL TNPHNNI IKTVLANTER KLNHALRRLR KVDFKKYWEI IRDHDVQDIL QPPNLVTYRQ GSYWKYDWNA GLAISTNLAD VMD PRGLNG CVETGRSRSE VARDLGLSYT RPLHENEKKQ LSHQAVYYER LAKFKMEQPE AARAMERERF VRKFSGMFVK MDIR SGAPD FPSTYRRLLG TKVVRWASKR HGPN

Macromolecule #12: bS16m

• Macromolecule: Name: bS16m / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Tb427.10
• Molecular weight: Theoretical: 22.077805 KDa

Sequence

String:

MQVTETVLAR AVIKRRSPQL WGAPGAPIIR MRGHHVVWKF QSYDLVVEHT HKRRNSDIRL LHYLGKHCPH PQKSLWSPDT PVAQDRHLF MLTTVDIDAF KYWFGVKRCR LSMKPWALLA KAGLLPPSLT QNSKIMPKPL FDKESLMRYY LANRKDEDVM A REKYLNYE NSMVKTEEER AAERPVAPYL

Macromolecule #13: 30S Ribosomal protein S17, putative

• Macromolecule: Name: 30S Ribosomal protein S17, putative / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Tb427.10
• Molecular weight: Theoretical: 35.632727 KDa

Sequence

String:

MLRRSLLAFP WWNFQTEHRQ RCVLMYGGAR TKNTHNANHR VFIKKYKRNA FPNRTRHHWA VSMTGVLSQR PRRMPWPYDL TSLIFNQPR QGSDKIGYVV GTSMLKTAVV ATNHMVYYPK FNQRVSRTKR FFAHDEDLAC VEGDLVHIKQ CRKISKYKHY Y VFSILEPN VEGRERLKLG LKAVPPPLFG YPVSRRIVKL NLTSTEGTQE KLAAAIQEHV QDAYRFSGPT PDQPRNRLAD PV TFEDANN MIAPNAPAAA ALDASDSPPL LDRGEYTEVE QDTRNKKGDD YWMNLQPKEK YDFKSFKKSP

Macromolecule #14: bS18m

• Macromolecule: Name: bS18m / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Tb427.10
• Molecular weight: Theoretical: 36.988691 KDa

Sequence

String:

MNRTGGSIYA HALSQFAVCR QPWNEYIGLL TKQDSTPYHT EPQEKPAYRG RKQGREGWLF GQQVQLHYHR FPDEQLITNL SRWRTGETV GDIAMQQFRN AQPFDIEDKD PQGVQRPSPE VYMKLNYKNP ATISRFLTRT GHMYPADILP LNPEAVVKLR V AKAQAIRI GLYPRFGNPF WFRSQKFRPK AYQENYDPTT YSTKRTVEHF AYNWVQTDRI RRYFRELEAV HTSGSASARG SG GGTTAEH KQQDQFYSPE NQPISLHRNN ISYMEDVGRS VKNPTVPGLM STKGMKKKFH NLYSSTSTKR MGFSNPTLGI KKV

Macromolecule #15: uS19m

• Macromolecule: Name: uS19m / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Tb427.10
• Molecular weight: Theoretical: 28.364604 KDa

Sequence

String:

MAFRNTFTTP GKFSTVSKNI VLLLIWRVKV FLRAEGFAHS LVMLPVSLYS KILLCDVKKK IVYFHCCTRK KSMLRRCPCV WPDGPTKSS VSIGTAFTLQ KRFLKIAKSA FGFYLARRGQ RKYPFLRRPH IKNTHSMNPS APYFWSFMTA KSQMAFLPEE N YITGDWTG KFFVSKRQVY TLQHATSGAK VRVKSFPSIF EFNSPSRWNI GKEMNTLTKP RMDLIDEQML TKKQRLDYVR AG LLPK

Macromolecule #16: bS21m

• Macromolecule: Name: bS21m / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Tb427.10
• Molecular weight: Theoretical: 23.087889 KDa

Sequence

String:

MLHTTRLWLG GYMMYHRKAM GTMKYSKWKG AHGGISHFYG RTPMVEEVRP NEPITLVDRR IMHYVHHSRL RHFQLFRSYQ EKSNSTECK LREGEMLRRR WHRRLQKSFI AFMQFKTMKV LEDQARLVNT YGQAAVNAAL GDPWNATDNV ARERKSAAVR R QVRALPMV NVVPKHVATM KQIHNDRFNY RWRVN

Macromolecule #17: mS22

• Macromolecule: Name: mS22 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Tb427.10
• Molecular weight: Theoretical: 73.064141 KDa

Sequence

String:

MLRRAYIQRR YPFNKRGPRE HKSWKHHVLT EPPKPLQWRD PKVWTRDLSV MKSFDAPQWD LWQSRPRSED MDEALQPFMD MPKSLKDRR YDIPWWANPF GAWYLQNILS LELLKLKSKT NAEKIATYRS YMRSLASGKD NTMSDDDVIR NIIKERWKTL E FGDRNAGY PCTFGDYIQF LNEWFKSLDE EGMQRLREHF DRRIRPLLAV MSPVDILWLE ALTQNSPHNK EQLQRKIAFQ TS LGTPEFF DMSKRLRYEI NEDYKVRDEL GPELFALWSK APERWPPERL SKMYGLDFTL VRKILVWHHF KACYDACVEP DWS LPKRLF ALEWIRDVRA RKHGLFYGKM RFAEQKITFY SDRFLFRDLV NRREASYANV WEMDDPYRFL QTEQDYEDYW GDNY DVYRR MFPEMIGRTG EPVQQYGQMP IWAGPHRQHA NKSEHNWMFA EIGVNVGHEA LKKLELDPTN EKRRRFVIRQ PDGTL RSAK MSEMRAWYWK EEWADFRFWA PQMEWGIENT PSQEQYQEHV PDTTDADFRK QRRIQSRPVK WFYESHYTRT GSFAGF QPL RFMQRRTERE VRWPDVINAA VQIQKRKPAA YIFKAIPEL

Macromolecule #18: mS23

• Macromolecule: Name: mS23 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Tb427.10
• Molecular weight: Theoretical: 36.869605 KDa

Sequence

String:

MRQCVVRRYK MPKNMGVAPR FDTWNEKYEP WEHMKRMGRL VGTGFYIPPE WYNHFRMFPP INHNFQQEKT LNPHNASEPT QDDTSTLSP ERVALRDELA RKSRLVASEG MRYYNIFWVR KPLDTMEKEY YELKRRGVDH GEAIRKVLQG FYSGLAVKKR V AAIQAEEA KLTGRFITMR EATVVLGVLA KLHKEQLTPH QVSLLAKEQG ETTQSGAKLT AIVSRTQPHV NKEASSPATS EA VGSSTEE SLSADALASM LSEDGEQSAV GTRYQVEVKE TANDSVRQLR EKAEDQTGFP DWYTGESPTY SGTS(UNK)CSRD GFALMKANK

Macromolecule #19: mS26

• Macromolecule: Name: mS26 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Tb427.10
• Molecular weight: Theoretical: 51.192793 KDa

Sequence

String:

MMRSSSFCRR QIRPYYNLPS KSEHGRKMTG FLTPYRHWMW KQNELWRNVH EAQFEHLRRV YKRQWLESFR VNADEYIYKY NITKAAQLA QWECEMKEQE KKRIEARQMM DGRQALKKKH LDLLREFHER QFFFWYERAS ERLQNMNLIN YVPHAQLREH I DKELDKYV AGKNEPYPLN FVGQMPFLED GDGNIVEVPE SLLSNHMAEH PDSTAKPHEP HTSSSISEAA AFEERMLRAM VS AKEEDLK EWLGDDSRAL SETIDDISRE EEEREADIRV ARSMEETDAE REVSRRAYIE R(UNK)KTGSRSIF RPPTVSEGA GGTPSAPAGD ANTPMRRRKK GKLDRVHALQ AHQDELLAKL SSQGLKE(UNK)VD ASSVPERGKI VQSRGRIRDK AVIPTH EVL MQKPELAAGS TPGARIQTKD MVDKMYHRGK YKKSGSGDKS DGEDL

Macromolecule #20: mS29

• Macromolecule: Name: mS29 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Tb427.10
• Molecular weight: Theoretical: 57.048836 KDa

Sequence

String:

MRSKTLKKSL AAMYMRPPVT CYTDACEAPV AMWDGAIPLK ETRKLKNGVP VRTVSRTYSH PPQLTPTQLS FNDINSMYCV GNDELIQFF PEGLGGRVFQ TMPPGHPRGF LYRKETHLLN LFVDKVQHWH TKRSVLSSLT NGRTGFIVDG PTGCGKSALM C QVVHFARS RNIVTLYVPD AKVWTHGEWC WPSTILPGFF DAPDAARSFL KYFAVANRAT LTSWKLRCTP KDLPTEQGER QP QNLYELC EWGHRAVAPA SIDRQSVCVK FLMDELSEEK KLPVVIVVDG WNLFSHETHF RYPHPDFLRG LASFNESSTD IDL YPQELP RIPASRLSFV RGLNKMILSG DDPNKFFITC TTRDFKPFDG ISGFPNVETD RFANSLDEYA PYDPEKDSHF HPIQ IGNFD EYEYRSFLRF LINSGELAGL GWGPLWHASS DFERKLYKIG FLSGRNPQGV VDHYHQELVW RYDYQRTRQK QYLLK RRME GMSRGASSGA VGVR

Macromolecule #21: mS33

• Macromolecule: Name: mS33 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Tb427.10
• Molecular weight: Theoretical: 21.244104 KDa

Sequence

String:

MVLRRWFPLL GVHRVGYTHP STLPVPCAQR WDLRLARARI FQEYIEEKAP GAWQLEDERS MSPEFKTFTG YPMRDMRPGY GQNLPDYIM KKRLPNNTHY ELFARRDIPN EDNAMYGKYL YDMTVHGTSL PSTYRMHKDI NKAQRNDRKL SGNRFKVLCS S GAKNPPSG WEPIPDATEE EED

Macromolecule #22: mS34

• Macromolecule: Name: mS34 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Tb427.10
• Molecular weight: Theoretical: 28.72818 KDa

Sequence

String:

MLRCARVALR ADPLNGGSSM TLGSKGSKLS PEPHRRRMPW TAAKEYVPGV VLNARDKMVL DGVQLLDIES IDRASQLDPL EVLRAVVAT REYNISTGKN IFQLASQATY NGRGQRFYRK EWQEGTYDKY VTLSAIDFDR DGNKGTAYGY ITFHGETTTR P VQVDFADV PGWYMDFVEE RAVPFTGIVP PPPSIGTDVP VDPHSYRLKA YPYYDAPNPP EFVERLLKDR GVLPDTPTET AD VDKDPTT SDGSVHYDGK

Macromolecule #23: mS35

• Macromolecule: Name: mS35 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Tb427.10
• Molecular weight: Theoretical: 98.277633 KDa

Sequence

String:

MRRWHRSLNP ALFGERGSGH YFSTTRARLQ LQAQDRIICG THGAGIVAHQ TAVNSSDNPM LSSVTTAAPA ADLSRTKPHE GTGTSERDP YIRTLHNQRS AAPESSVSQS HTVNAPTVDE CEMLAERWGT MNYWHNDTFP RLVVFLKKLL VPDVSPLSPT A ESLLSMFE KVVIPKLTSD EEDRRKLVSL WSETTLQAEA AVTKFLFQRG SFESMLHRII TDALEKMSTL ALGGQEGNLA LE ALKRQTL FKRNDYIQKR LIDVVSNSAY LGYGDSVWQV FFAAVEANEE NLLSDRATTD AIRAAWEGVM REDVVRLPDV TGV VALYLT LVCIRESGRL VPEELKELSS GLEDGVRPGV RKLQQYPLIF LHPTVKRRFV VKAVAEILHN SSSNAFSNML RENG LHDTA REVALCEAMN RNKELAEGDV GDAVGRFVSK GEVKTLLSSL VSGTDAVVRD AVAGIFGIGT TITIDWDAVM QNVDW SNNW QRLATALLSN SAVLSAIVKL VKNAIGAKGM SKHLFTDEYA DQLQLILDAR EERAASRKQR IENIAQELSS FERVDL SCD LLRKLGVDMT ELDTAAAATR NMNVVQRPCI EDGLLSLVLE AVTKRHPNWV KAGVIQTTLK DPFDALRWMM HIFIRLS YV PHAGAATIAR LSRRRIGPIG LEPHQFNVPA ELGFVEQYDN LQYKRYDWQG WYQRMLDVHN RNVSLRCRIC DLQRLDGN G VQFVDMQTER RLRILAQHRV GMGVLKLDAD KYEDQADNVT FGTTKLSELL ADARKAQLGE EYWPSVELKV RKPSGQSKA HYSLIDNERI EKRSGELYEK YRDAKKRSLF VTPMETWLEV KGMQVRKSVD NADEDGYTLD ALQDMMDGDD GDKV

Macromolecule #24: mS37

• Macromolecule: Name: mS37 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Tb427.10
• Molecular weight: Theoretical: 24.757896 KDa

Sequence

String:

MKSSDIFFAY RLTPVVFKSR QHDSGVNQYG LKPTNAYDYI NPTNLINFGR GTTFDNLGVR RAGRGEIDSS PSHSGSPVFT QAKLIGLSG EEQLTMCQSE TMALRLCMAK AGKETCERES RALDSCLGRV GHLRRAMSEA CWEFNDWFIQ NVSDNHTKPF Q HRPHDWRH FYAQEKLVRE RQQNGHAYGR RPKQFSFGAR YVKTDGYGKR PRLPYNK

Macromolecule #25: mS38

• Macromolecule: Name: mS38 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Tb427.10
• Molecular weight: Theoretical: 28.2406 KDa

Sequence

String:

MRAGCVACSR IPKLGIAALG STTTSMQATC QETQELRCAT TQMAPAVIFP LPPPLRGSYI DRKPTAASFN EHHATASFRH HMIASADVS HRPRHFYFAS GTRNDTGTRS VKEGERKQLK QQTNVEVDSV AYGDQLDELS ARWAAKFYGQ VTFGPRNYPY P SSRWLARR FQMKKHRIIK RFRFRRYKLA AVANLPFAKM IRVGMLPELK SSKTKRGDVV DPTLSGQLVS AVKNTGEKRK GQ RTRPKSK YQV

Macromolecule #26: Protein FYV4, mitochondrial

• Macromolecule: Name: Protein FYV4, mitochondrial / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Tb427.10
• Molecular weight: Theoretical: 22.421469 KDa

Sequence

String:

MLKRTLTVLD QTYGPHKSYK YTYMPDPRKL APIETTQRSE IVPQSIRPPT SYVPNHETFL ERADIHRLRP TSDFKASFKD WNDLFTCDK RQLRVRGIPR MTRDAIRTAV QAFQNGNPPE RFDTKEEWLY YKQFKTVDYS YRVIPELPEK YRPHQSGIDQ A PLPDYREI NKMPQWAECE ERRQSKKTV

Macromolecule #27: Superoxide dismutase, putative

• Macromolecule: Name: Superoxide dismutase, putative / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO / EC number: superoxide dismutase
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Tb427.10
• Molecular weight: Theoretical: 29.748666 KDa

Sequence

String:

MRLCRRLSYH AYVPKGASVQ MGETFMPSNI ATAVFRETEA AAARADKTKE ETGFFSQPRL NYPVSSGIPA LFSKDQFDMQ YSLFHRDVV ETLNRHTLGT PLEGHNLETV IRKTSFDATQ AVAHTAASEH FNYCFFYKSL RPWGTAPPPR LREALQLQYG T NGSADAMD EVRRIVWVTA ASHQERCGWV YLVWSGVHFD VVEFPHGSCP IASDLIPLLA LNVHESAYIL DYGTSGLKQY VE NYFKACN WPLAERYYLM AIGQVV

Macromolecule #28: Sod_Fe_C domain-containing protein

• Macromolecule: Name: Sod_Fe_C domain-containing protein / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Tb427.10
• Molecular weight: Theoretical: 48.59041 KDa

Sequence

String:

MLRRTVTSLR NARELHRLLP ALLPLLDPPV PTGASVCAAH LIVATGHVSK QREPHMSNCP VLERKIPLSE IASDDNFLKH PCVPCLKHR INFLASEDFC YDFVERCGCG TDGALPPEWR CIVERQFGSM AALVEELVIH ASNRREAGWT WLVYDAQQAG E RPLLVVNM PAQRSPLLLG LWPLAVVNVT EAVLVEAVRA ESSGSVAAAP PPPWCRAARA SLYDTKAAAE AFAMTAPMSE GV GKKLSEI REKVARDAVS RMNWGFILQQ LKKAETYYAS SDRGEKIRVH RMKLEREAVT KAMSGLRDSG ATFLTHDTVE VTT TPSANS EPSSPERVPG VQAKQPQTEG MDPCKNKASA EPDPKCTQLP DGTWQYSYRN GDVTLLKPDG TRVFRKRELT TTAY PDGSM LFQYANNTSI LDRADGVRVT TFEDGTTKEE MIRR

Macromolecule #29: ECH_2 domain-containing protein

• Macromolecule: Name: ECH_2 domain-containing protein / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Tb427.10
• Molecular weight: Theoretical: 137.898953 KDa

Sequence

String:

MERRRLLHDG RWFTPPVLYG SARRGLKTLD VREYRPLAMP IEFRFYQRYA NHPNRQSGIQ FLTHYNTHQR FRVNKDYIDY MHWGKEQGQ ARLPHRHQRV AFDFNDQLHP TRIGTPEDGG NANHCYPEDG EDGSYAWFAS QDPTFGHHPD LSSSFDPNYR V FSHPEHWN KMFTKRRPGE GDIDLSVLPS RSLLGPLMAH SDTKGIPYFK VDNRGHSNGR VPGVNAPFFG EFDQKMMQAM SR PLNANRT ITGNDGRFSK TIMINEPQCN QALSAKTASE LSKEIDKATN AVYSKLSVLE AAQSGLTDYF CGGLNFEIVG FEL HMAAML RERAAAILER SGAGGLPSAS SATLSSSSTT RRCAMLLEKA DEREVNKLLR DASRYEDRVD DALRQHASLI WRVY TAPRP LMTLTNGKCR GTGCGLSLFA KYCALKDSSE FIFDGPNVGI TPYGGMTRLL ARPETSLKYP GLAEFVVLTG ASLFA GDAL RLGWTDLFTT IPDMTYHIKE WFDTTEHMHN DAVAWQLGHL LETCFKMKES HSSAMERAAI TPTRARWVED AFADQP SVN DIMRTLTEIE QLPFQSTHNT SDESHITPYT LGSVAAGVQR LGEHRLRYTL SPWDITPPED SVVLKHTSEM FRAYVLE RR GATDVVTHRD AERAAAWSQQ RQREYDAYCS LKEAPHPRHV YARLEGCEGK IVSFEFVFEI SCRRSGPQGT DEVLQATT I SRLKSRILDA LGMPRDRNID LGWYLPTLDT CPIHSDEELM QVLHTDPGIE DPKEKLRYPP IYFIVKRCTL YFSEWAYAV KHQLLLQSPF ALRAAFGLLQ EVRGDGAAEN VMPLAETLGT EFKYFTRLMR RPDFYRVGVH TDKSVEKWEE IKEERRHNIH HTHRPTRPL PDFEEVFERD VEIDGHRFVL RPRWNPRTLQ DVDDSDILRL RAPLTYDMDS TAALHVPTHC GKASRLAGML Q DAGGVQVL DGLGEVDDKG EPKVPPLQSN ASVPTNVSFY EMARHPWEDR ASSWRRDGFT EGSLEYFESQ YRSAERAVYD EE GRGVRNY WPSRDAVEGI AQEEENDAQL LQERLFNTLE TAGTSVEPWA RNLRLNATTG KFGYKTEIAT QEEKIYDDEY YRW FIQPGR HPNPSGLTLN GRKGQFEEGH SRDEELERMW RKVVADGNND FAATTSGMPV YDEDAKNAED SYAAGDDVDV VTTD ANVPV DGDDSS

Macromolecule #30: mS48

• Macromolecule: Name: mS48 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Tb427.10
• Molecular weight: Theoretical: 201.787141 KDa

Sequence

String:

MMRRVASTGS NGGHCYVGSS LWADSVYMEV SSITLQKRFS FKYATKLQHD EMRQPYYIHE KRYGIFSNER NIAKARRGLP FITPLYTKH MNLWDTDTDA SNNRFFRGYY YGQRELHQLL GRPHSLEATN ADGSNDLSTY EANTSQLYKG IPRPAITNLH Y EPAWRYTL YQAGAHGAQL SNPRSPFTAK VLGDELMQVR DIKSVEHCKA WFDRLQYLIN LHYEAVGDIG EFKSRHTRHV HE FFVAFHD ALSSLDFRDT YLFDQFKAVR PPELSDLFGI FLEMEANYVH EDYCPRCSLP YSTTRYCGEG DVDTPFRKHR GRW APHQKW GREWYDVVAR RAEALWYRAT EDPYFGTPQH TQRQAEALLK VYVQAKQRGK AIDFMNKLRG SVEYLTGDIT ITTV MQESY DALLDTTPHP HLLTNGFTLE SDAAKYTGEV NKAPLSPLQF RIDMEMNKYR RQQKEEGVVR VPPALWRLDT SAIVP YKVD SKTRRIVNWR EVKEGIEKSF LSVGLPKEAY TGNEWREMLY LHDVIANREA KAAVLEQQQK LDREKLKARE RASLKG EKS SNSASNGIGI IFPDKDGYQV FFVDEASLRP FGIGASGTLF KAVARVYPSP AAVPYDDPVH GKQFLIDTTD ETCHLFG GF EHGDRLLLRA KSSGGDVDGG NNSNCRNTGS FTDEEEEVIV MGVSTEGSDA ERMLCAMHVD TGKQRERGIV FLGTDCID I RERWESVRYA ASAYVKGRVT LLESQRTARE EFMGQVVGIR DGVLFVQWRL LKGGGSVLDR SVAVPIGDST QVRELFVET KVLGVEPLQS PPSWRTPFRN DFAEERLKEL QRAPFKREKY VSLIQGKYTP KVKKFGYTQH TTVDDFETKE YKDRLLSKQF FQNPQAFEV VPDRNERSVQ FGGKWEYQRT HGLPTVDRNE LENGWSEVEP ISDGEMKVIE QALRDISGPR PGNFVKPPSK T KSLQLSES WWEPLGYGWE QHNEEQKALC DVTEQRLIDG SNLPFGGKLP PFGTSYGMGE RMRAIIEDYS KGFGLGPHGH SP THDTIHY NTLNAEGERV RDLGYTDALG RLFSEKLGDR DVHQWAVESC ADGEADVRQL LLSLHEWRER GRPPSLLLAN VLS KYLEEE IAAFNKGIPE NAPKLQLETS DGTLAHSGSG GSRSGTMWAD VDPTTFALHQ ASQSTRSTRC DEPFILHLVK RAKL GECVT NFTDTAYIAH LESSVINEFH LALTKLVGKG ISPTLLAQKT GQLHRGSVRV GGNVVPFVKS RELSRLLERM GLTSE SIAV ITRGLANCPE QESVGDDFAV PVSLILSWDG PGSSGGSGSG DRRLNTTAAR NEQQRKGSAA LSSAIRQLSQ HQKQRG SAS GRGWQNEDKM VVHVLEELSL RNDGLVMDIQ YAIRENKKNR RLRWEFVTTL LPVFGGNEAK VEQLYSDYCD GKYVPNI TV AVEAFIAFLH NATQHPETYS ASDYFDIDDG KSSTASGTGD QPSAGQYTTL KLLDPLEGPF VFDNVKVEYI QTVERFRR H GIRAGPVMAP ATGFIAANCK SLNYFTRRQE EVVYVTNDSD QGLRRSLENS AYHKTIAANP ALQYLLKARR GAALVETFN RFFYRTMPML NFYQNVLKHY SETIQPLRQA AKSSTRGLAR ALESERSAAM EEFRRNSERY WRNIIEGRSV EQVVLANGES NRRTVGGGA GEQLQKPQGN RDAVRTNFTG EATDGSRKGQ RQHQQPPLSQ QQQHSRGEGE RSAVHTSPRQ GGSRGMADLL S KLNTPKTG SGVKGSTAKN TGNNRRGPKS

Macromolecule #31: mS49

• Macromolecule: Name: mS49 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Tb427.10
• Molecular weight: Theoretical: 137.234125 KDa

Sequence

String:

MIRRRVCDGA RLAPNIRATF SAVRYQSGLH TFIRDSKPSN FSSVRRSENA NGDATASPGA TAGENPASSG DWASHMQREL FGEVDPLGG QAHKDYYRDV TRGYSPQYAP RNFANGGAVA YPHIQSPYEY EEAAHRRVWL DHDVDRMREE FTQHRASLRS L ASAQEREE LLRSRAAEYQ VANTVHESES VHPIQQLYNS GGTSRSALKQ QAVADRYSIA EQHSPLPLTT GVDRDALDEA QR TKDRILN DSFTAENLLI THGLREKEKH DFTILQRTVR IPFQGYDMDR FLAQQKGTPY GAQQLPPNVV PSSMEEAQRT LRG SSATAT PLVDAVAQKV YARNTVVDRP AIGEQLTEQI INIMRASRTT AEQQREEERA QRFGLGRQGA LVQDGGPDQR TLKK HTNDE RIVDAMLFQQ NAYRKTPTDE HWNPYIRRST ENGVGHLLQN KFDIMRREDR LSKGEQDLTE RNTIHYGVPI QQIVD EFVF RHRNARGERP LDYFKPFPNF RALRLNRMYR DVEGFSLMKQ RPEFLEWELF TRYRQHHQQR RRLALLHGLE PVANET AQE RDTRRHRLDE ICERTPFDER EMRVNDDEMR VSVETLRSWF GVYMLPSPTV VNAVLGGSAS VNLHLYHLAD EMGTADT RE HVLSSRYLNR LLLLESYQNR VGRGFMNHVV GRAPEPVVPH EQPQEVLRHF SAEERAMYEQ HVKEQTSRQL GEWERAMK R RRWLTDHQQY GHVVSHGLET SVVDLSHTET GAVLTVSTKA YEQEIEAVRM KTNATIKVDG MVYNLLPNSE RRVVPLTVQ LDSGEKIDMT SEDFDRCELE AFPRNLNHAL NYGIANYAYN RGNYVETQDS IWEEQTASGQ EGWSPATHAD GLREGLPVRA RRPIFSSSA EQRIAGGPQR AVIIQYHHQP FFNPEPRLVK VAFQCDGTIM EVPISDVMIW QRRYHGPERT VGDESRRYNP A AMRRYVDV TDPFNEKTSN TEHFLDKYEP KRNADTVADK YRTTKQITEI DKWTRYDSAR ADNYRPLSIS HRRDYIRMGY IP RYTPWEW IAIQEADQPL IAEQIRQDNI GTSYFFSLNR YWRYKASPHG YIRHFENEVR DLLQYVDGVT PWKQAQKIRT YWE VRSHHP MPQFNRPEVA MHRNTVGLLP AHMWETDKKT GKVKSVKDSV RDYQTKTPYP KWVQL

Macromolecule #32: mS50

• Macromolecule: Name: mS50 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Tb427.10
• Molecular weight: Theoretical: 131.71925 KDa

Sequence

String:

MFRVRRLAGF IQCSPCGKMG PLKQQTRRYT PIWKSDPAVD NVAPLRDEDE RRALWAEVGP ISDVGSAVTA WIRFGNDPVL HTAVPTMLG GKFRNQQREK ESLLPNSSSP FAYVEDYMGT NLVFGSPVHA KESAAVWATY FERRYASRLR LSRRTVANYV G LINSPEVF DDESDRPETR WSQDTFFREC AYLSEKFLKE KVSNMQQFEA ALKRASPEAY LAFFDAFQQQ TQTQIPLPSP SV WHYEGER RKQWAEKFIS ISHKAQAFFK DVLSEDVKKY QEVPGKLLQK VKPVLADVGK ILVKRHERWL KGRVWTSLTE EER EAYCMK EVKRQQMQVE DGEFDPMMED DVDDTELEEW QREHDAIMKL MNSPIDGLHF TTLELWLHTM RCEELETEHI YTSA RVRAI QVAARKKLYD TTSYEEVIQA VVESIARGTL DLGAGVLRPH FNEVWCQLNY AKFGSSTITQ HTTTSRRQLL FFHAG SLKD IAATATLYYA TKPLSNSLDY ASPYKYRRSL ITLCSNYGVE TAYTTQRPLL RSAANLARAE DLIHAVVTAA AQPFGE RRR AATRDLHMEF QRLAVPVERV IVANPVSALL ESGADPDEKP VEGEKVNMWP LGAKRVVLYK WSAPNVEKLK AMESDAA SA VSGSSLTAKR LREIQELKRR GFLEVSLWRR VTAQERKQRN EIVEAKKKQV EEVVRTVPSL AHLHQYATSL YSRIEERV A FPTETSTVTE TTNMKEETNK PLEDSEWEFA VLLDDRVLLN KEESVELYLP YRDANGELLA QGEYRALVRA FDLEANPNL HPAYCSVGYS ESFQVFDALP QLIAQFFRVK DATAEAAGVT HIPAADFTPF CAFLRDAGLD VPLRCEFEAG QAVTTDGDVY MDYFLQLLR GEAFHQSHAQ AGLTEAQRAI EPLCRAHWVV HHPGADESEW ATARRSVLDH AMQHEREWWF PNEMLDVKDV V TGSTNGLT PQMYPAAVRY GVELCTVLTA EGKFVDERGS GLSARCVVNG TGAAESVVFD TANCNGTNTT SVEDALRVAH GA LRSAQDR HNTLAAFRLG PLSKQSQVLL FCGVNAYEFG GKYARTYAYA FEKAKKELEA TAASGFMAPS LSHEDTERLS DQP TTSPSV DRFASTTHPE QRKAQFVPRV GPGSTPLEDP AADQKSEWS

Macromolecule #33: mS51

• Macromolecule: Name: mS51 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Tb427.10
• Molecular weight: Theoretical: 95.080992 KDa

Sequence

String:

MLRCTVVGHH RSGKARAFVF RDPSLRMMRA GSGYQQLRRM GMPMQVGMGW RKVDSFHANT QYQHAWPLLS HDDLGNSDQS NNTKNIMYS MYMPKRNKGT APWFRGADTY SVKYCEQGRY EYQRYLMINR FPSEYKKHFL SFLSNIRMSS GSATIPQEAL H WLLRMIVD NFNPQHVHYI AAMKTLQSAG ELDMARDVWK IMERQQTWPC TATICAYLDV CVEAGEKTWA MEAWNRYCTE LK FLEPGEV DPKPISRVPF SLTREELLYL PKWKKHFDHD PNLDVMDLNR FNRTREVYLR MAQVMLAGGE RNAFQHFFTK LEE AMLNKP TPVPEPPNPH LVRRPRWAPY EHCKSVHHSP WRLQNNGRAL ALGPPVTIED EMQSRFFSND QFLVHSVKEV LRIV LQEHK RAHPTECTRC KTEAFFYKTK DADETLKFCD DLIERLFASL GVRLSNLNTS SLLSTILEVF RVVGKESGAA LLQRA NEFL ERKASLGDAE GSRENLTASN YLQVLSGFAD ESAFVYNTKK DGTCQYKTGF DPRTTMRHLA DVVQEIAGNP HVTWAA DMH LQVVETMVGC GTMKANDYFV RNVLRQFSWD SRFLEALYVE YRRQDDVDMW AELTKRALVW TARYNAPASE RLRRLIE DD YDTIRVQTRT FRELAVFQFR DVEERRHSRD VVNELPNPWY DYVAHALPFP DRDAGYPDEY GDLGQWRAPG GPGSPVRG P GYYAPPMEGE HQRGYTAEWR DLRNPMRPPE FPTPWERKYR QYARGQHPSY DMVYAGPMPE IFPMRRDFRK PTRWDFHDI EKQGKYRTSG PY

Macromolecule #34: mS52

• Macromolecule: Name: mS52 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Tb427.10
• Molecular weight: Theoretical: 84.938164 KDa

Sequence

String:

MRRCSFASTC RYRSADDVPL GLGSAYVWTC RNITSRGQFN PIHNFSYAME RGVRARDVKA FEKLITNPGP LRVAYTPDYL DWLHRCYKA KGTYMDARAV AEKKFNGNIV SSELSAAVNR REGKRGDTRG DTVDNDHHNL PGAPPPGMFL RPAHSFRRLA G ELKRRRAQ SILDEVARAQ GMLDLFERQP HFPAIHIDRC SRFHLVELFK EMVLERSLDS NMIWEKALLY RAILSERKPS YP TSFHYIF TAVEDTVFAP TISTPMEMAG SGGEGHDRSS HPLAAKCPTL EAYYYYVYLV KKYYIDNAVE AHVVLRCHRE PNA ADLLFS NPPPKDDTEI MKAVELLRNA DIQRGVAAAA AVSDPTLPPG GEGSVIGNSD N(UNK)KNSEK(UNK)SE GSRGRP ARP PVLPGAYPPI DMLWRCEENL PLLKVLLFGE FNLIVSENPF VKFPSAHGFL TRPYSTDSSR TLADGMSLAN VMAEKRG HL LPSLPRNTAT SIDARAQDIR RLQQKHHRDD IVSFQKLLRS THAEDSPSAF SSYSDWSYFN PRAVRAEERD RLTRKAVE A LKLYDSATND IYRHSFEDVQ ACHTQRVTER DRTMPPYLPT LPHFVAIIKK DPHISFLLHI GLPDRNSSEE GSAKHKELE KRIYYLARAL YHTALEYHNE TVRRVNRQKV NVAASLLDNF VEQEWTTILR DKHDVTDVTK TLNDTQNDKK QLARRLGRYM LFANRSLDD TGFPTDARAD DYTRWMAPPS VGKVSL

Macromolecule #35: mS53

• Macromolecule: Name: mS53 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Tb427.10
• Molecular weight: Theoretical: 75.338914 KDa

Sequence

String:

MSVSGVFSKG RGIGHEATTS ILRYIPRARV PWQPSRFGRE NLTAADMARL WSRGRYRDGP GNYNSGYCTE RTHVLEENTV SIIPRRELE KYMPDITIGP KALVTPVSLM NARNGHRVTH DLLHSYDPHI GRLGKPAVVD HDNITVEDPN RVGLNAATLD C RGRIYRWL RRGPFFQVDN YFRRSVKLNR DGTLPTDFVH EAPLMRKIIR LAHRGHLKAA CEEYRRVTTV PPVEVYRALT AC CVPGAKL ADAVSIFEDG DSKLFYVSRD GEVLHNLMRC AIAARHRARI MWVYNVMRGR FYENVVVRAE VDLIWRYRIA MIA LEYLLD HECAEEAAAI YSYLVEEELL RCDVHVRVGL HMREAIAAGK PITLNNDVMN ATSLVRDATA VAPEVARELQ RRHA QTLQN NAVEAVGAEN VREGSAPWSI LGPLTAIGPT AEDTMVWLQQ HYGDVDVMSI MRWARFRKGK DLMAKDRPQY LARAA AWIE LLSKRNREME EVPLTYMRKS KPLVLDTNSN VRVAWQTPLM RSGGPPRLLA REEGYVFHHS NSSRFVEETY RHPGES LQS RYLALQPLHT EVSAKEDFQR LYYQAQKHHK QQERLLPVST AAIPSRIVHH SVMSALHGVS GKGEPANRSL FT(UNK) NKSNDS HSNTGVASGT SACTDGAGSA TPEF

Macromolecule #36: mS54

• Macromolecule: Name: mS54 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Tb427.10
• Molecular weight: Theoretical: 72.046031 KDa

Sequence

String:

MFRRAIPLLS ANIPRSVWDP AQHNPNWSDS YGHDITNRRA WPARKWTVGL EPCTPREWLQ FSHRNLAYAY NGALRACHSL PSMLLLYKE MKQRGVKVDV DTMNVLLTRA ARHEHIQVDD VFLLFDELVA LGARPDLAAA ETLHTVLSHS ASMPEEWREA R RLQLVELY NNLAMEEVER LAPHRADRLL KEQMKRFRGN LQQLGSGLRP TVYCRYLHTT HTAAVLLEEV HNFLWELVPN DH PAMEIPA LQLRVPFVAS VLRRPSVNPG VSLASVSRAE FGDTDVCAVF LAAAERMVDA DFDDQRPVSE RRLFLSLLTM ISY SGVLYT SDLMAQLMEM VKYSNNDETR DSDAQRVLRY ALRGSSAAQD SASRTLWHSV EKVADCRVVG RYIGARNPWN PIRV CFDEQ GVFKAYPIST TTTTREVSPP EGNGAVTQEQ RASCVEGRTL EALNMRWDDV RRLIECTGVL VTPPSERCPQ QQKME VFTG MAVYLRTVAT GRRYEGGEDV LSDGAVATSS CEQRRRGTLF AEGYDFDVWV RLFSLVQEVR HDMEKFMADH TLQCVE PEF ECWEALLVTL RCALDFCVVQ MQGGGARGTE REVVERLFRD VVALREELIE ESRTRFGGRM RVLWLQEA

Macromolecule #37: mS55

• Macromolecule: Name: mS55 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Tb427.10
• Molecular weight: Theoretical: 66.67668 KDa

Sequence

String:

MLSQNVAKTT VPSYYMIRTN LPHRKPQNQW EGVYYYSGIT KRQRHLILLH RKREREAHMR SFNISRASVL QRLEQLSGDR KQESLPPHV RLDLAVRLAQ HGLYQQATPI VDELHHQKAL HAGHYALLIN ALACPRLGQR ILHCDAQCDP ALTYKLLGDE N GEERAQEA YRWFDLALTS LAVDCGGRTQ PSHFVPYLPQ GTAAASHITN ALMRTLLTCG YTHVAAIPDS VYDRMGSMGI SP TISTYEL VMLALSLQGN MVEAESILSF LRSHHSEHIT VESFNALLLG HREARQFDCC DAIWQELVDR RWPRASPLTA ELY LRSIMD HANTPTSEPL QSFANINVVE KKKVPLVLAQ MDELGVPRTH LSRVLMDEVE DSLRKFQTYR SRFYEWGRAV KQFD FIEFR RRNGWLYDLH LMKCTTKQVG PLRDFNDPDA VQGAVATAEI PAFFNERPAW ERPPLEETLY VTTNKERYDD VRGGD IYYD DTRGLHDRSP TWMNEVPETR YDRLYGVNHP DIAKIGIRRH LNVEYVNRKE VVERDAALMK KTLSSGRRLR HRVESS RTH RNAGSLSGIS STAGGGSR

Macromolecule #38: mS56

• Macromolecule: Name: mS56 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Tb427.10
• Molecular weight: Theoretical: 47.306836 KDa

Sequence

String:

MRKFCHFMAN CWNSARSHAT YGAVPLTHSQ VTSVYATDGG KVDELGLLEL VEERIFSWKL NKWEMRIPPN LPNDQKELIR QEQENLKQI LSEWRKCFGA LNADILQISS LTGVPKDVVR EKNRTWLQEE VAKLRWMGEV NKAALLRDAF MRLEAFGSRD F MFMERLCC IYGLARQGTF DEAFTNYITE DPVTNDIFVD ERNPFKELVA HIVRNYSQID IIYDFLGFNY SEGYRSSLRR YM EYLQCKT AENVRASGRL VTGDKGEHNI LFDYCVSRES LVSGDSCQGI IDFLYINGND VTLIIIASDN PWLRNRQLPH RRQ MEGIAR RVCFVLGIPP SEVRIRNLLL PPTYLDKGSI VRLNDIVFRL SNEQSNLLIP WLTNYNKELD PKDVDYTALA KTTN EEEWL TL

Macromolecule #39: mS57

• Macromolecule: Name: mS57 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Tb427.10
• Molecular weight: Theoretical: 45.956293 KDa

Sequence

String:

MFRSTRPRSV GYTPVNPDTS PMVAYSQYHW HYNLPQGMER PHSVNRTFAA PFQSNHSLVN KYRGVWIEFD MHPAFSVALE PQLRKLPRG RTLPKTPAEE VIADYTALAP LVDDEKTRDL WLAKVFQHCA FQRCGGAMEL WERYCHQRFT AEGATAKPPL S LVKSVLFY CNKTDNSGWR ALFDRCLKDG WNYTPLFDTA QWSFMLKSIG RMGDEDGVRA VLEEMLDVQA DLDRVEARSV VI ALNAVTN ADVYEFVKKY LFNFGERKVK FLRTTYSDLR GHGAGKLRIP LKENDNMYYH VCWHSSIRSP RQFSPRQLYF DYT PSTLGS SSHNPNAKID DIVKDKIEKW KAEGLLPEDY VHEDRVYDRS AAFKNVARQE KWKKMPKILK SKRMGYTGDP

Macromolecule #40: mS58

• Macromolecule: Name: mS58 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Tb427.10
• Molecular weight: Theoretical: 35.62391 KDa

Sequence

String:

MSFRYTNNLI GALKHRLLLE SSYREIASRK FIGNCRGVEV VCSGYGTVLA VQLTDKAVWE SFYRKGGRPT VSGGGDVSGD AETGTQGSA TTTGATGSLD LDKLAESIKT ALWDATRKIR SAKEAALHRS LSHNTRMRAS ADLKHWYEED ANTLRPLAFE A LKHEAATP WMQLVQHGKK EEAAALLKEF EQKGDAAEAT PTRVKDDRVK GTRTELSNRE QPPLASTLKA EDSNPATIPI GS VHPLFTP ALVQIEEAGG GSVSNEAVCR AQLWELSRDE QLFWERVELI RKGQVASIGS SHKRGYADEA AFAKDDTEEK VQL RFTQ

Macromolecule #41: mS59

• Macromolecule: Name: mS59 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Tb427.10
• Molecular weight: Theoretical: 35.395676 KDa

Sequence

String:

MRCSCAFLDK SVFAAKRRVI VPIHPTPNFP AHFIKSAFTT DPLKEKQKAR FSSGGEAMRE VQDIPKNLEG ERSRRDLASR GDTEFQALV EFIEGASYDQ LISGRRFKKV YDVLSENDDM FIWLCHTAMS VLNPGDMRSR LIYNHLRILA ESVASGEMTQ R TAFRFFES AVRSPAYREI AKRQLEGGAA TRLAGISAAA DVMRRMGLTR RPMSSYFELY QRIVERSEAM TPWGFPPLFQ FE ERLSLEP RLKFFSRASQ QSLERRRRGH VMTPHTTLHG RRIFWIPPTW NRAGRFLGPH VTLYPGMTPD

Macromolecule #42: mS60

• Macromolecule: Name: mS60 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Tb427.10
• Molecular weight: Theoretical: 34.546641 KDa

Sequence

String:

MEVYRRITER VRGALWAHHG RLMSERQFHR ILAFVYNNKY EYQIRFDRME VVGREWGGRV EVVTTPAGFL KRVRVNPCVE DLTLYQQQQ LILSAYANAC ADGRKLMEQA EMNIYKQFLK DLKPIVMGIR DNPEFYTVSE NSIETIGGVL QTGDSNNLCH T HRTIPAAK ARQPADEVRA RQEWEQKWIN SPRGQSWALT LRGKTYFALH GPQYRPRGAP GAKKETLPLD IPAPYTAMDE KR LLKKNWM AYLDNKHVAE VMWTRVKIAD REKRQRLLQE TGRAWHRPIN EEAINRW

Macromolecule #43: mS61

• Macromolecule: Name: mS61 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Tb427.10
• Molecular weight: Theoretical: 33.48234 KDa

Sequence

String:

MLCRTFLRQF RMSGGDMFVE YKVLSRDHRR SIRVEDAIVD PTFKRTVLPL GWLELLRSPS LRLPTGYFVE ETVHVSLPNA TSNGGKKEA RPQKGGFASG SPSVGRNEAN AIIAGPVVLY ITGQSVPVVL NPYFVPEGTW DMRTRDGELD LRLGMDAIEQ C TLFSELRP GGLLYGKLPE NPNVRRNESL RATLGRYGMK CDLAESPLVP RPWTRMRYMF IDELQRGPKL TEFVGHNPRN GT PWRFSQN TKYFRIGIWR DTIRRNDMNE GLHAHSSWQK SPQQSVPEVR FLAPYP

Macromolecule #44: mS62

• Macromolecule: Name: mS62 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Tb427.10
• Molecular weight: Theoretical: 32.0681 KDa

Sequence

String:

MRRFCTARVS SYVKRAPLLL PRGGRRWRSG AGTTADGGGE KEYIADSFES TAGSNAYAAM ELAAEARREI HELWLSAETA LEREKRVQQ VAALIEKYKL DPSTPREADV SRGLGDAFDR LLLLCLPLGK TDAKGTDNLE RLMHLAGRNG RELSVRTIQH L FARTDSFA EALAVFYTMR RCHVAMNMEA YYSMLYSLQR LEEEGWGQHF RNEYEENGAP SEQAMDFIVK GISNALLPEN KP WLGRVMF QDRNVPDRRY DTRDFDELDT AWTQRYKSGT PAGAH

Macromolecule #45: mS63

• Macromolecule: Name: mS63 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Tb427.10
• Molecular weight: Theoretical: 31.922178 KDa

Sequence

String:

MLHGTPVRRA SLRYRRPYWM MFLKGVDNWK IYTVIQQPDH QRTEMLYQAW LGGLDRPYTR PKCMANQPLW LSKKRHMLRK ERLDGPETP LEKYVLEWHK KFHSFQGTER PTPDDLHTAL DLVERPLDLS YALQLLGQCR NLNNIRFAKE TFLVFLEACL R VGRRDCAE YALEHAEPLG FWFIDEDHRR YLQGEQTWYK LSPLDNLYYP VEENAKLNEG RKPITRLSPA TESPGSGTAI SD GEPSTDV EGETTVDDEI AQLEAELAAL EREGGGK

Macromolecule #46: AKAP7_NLS domain-containing protein

• Macromolecule: Name: AKAP7_NLS domain-containing protein / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Tb427.10
• Molecular weight: Theoretical: 30.742207 KDa

Sequence

String:

MLRRVPRVLQ KGERGLYFPI NHRIVDHRIA PGVTVEETDV QSRYRRELRT NFATGETRQT IAPPWSARER PTHFFSLRLP SRNSVTLRV KEMHNHLLFS HQQCAPMLVS PTKLHITLGV MTIPENSREE LVKNIQQSMS ETFACVQPLQ LRFRGLGTFD F GRVLFIRV VPEADFGRLE RAVCDIRRRV GGVLGVDLKG NPHDSYVPHV TVAKIRPNQR QQLGDTIPQS LWADYQHHDF GD VTLSTVD LCRMKGSPDG YYHTEGAVHL S

Macromolecule #47: mS65

• Macromolecule: Name: mS65 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Tb427.10
• Molecular weight: Theoretical: 30.617381 KDa

Sequence

String:

MFSESLCILS RRFRYNTKFP ALVSYNKLPW EVVNHETPQF HMHVAPHYEQ LLTLAASSPV PHIIGSKHID VPREHRLRLL PGMLYLLDG DTLPGEFTIN RVLDPTALQY YGRLSSQIVT VEAVRMLVPD DLRLLCNCIT FKGPLHLPVA PYASLASLRG A SQGGTTGS ETGSNCFTLY HFVRPNRPPK ELQLEKYYIH APCVAPLSEF ASNSDERGNW RPRLQAPKRT RRATPLPAYR PP QSYLMGL AERLAVVPGG CFGRRSLMWG HWF

Macromolecule #48: mS66

• Macromolecule: Name: mS66 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Tb427.10
• Molecular weight: Theoretical: 29.960182 KDa

Sequence

String:

MFNRNFILRA AEFKQKSRWS SVWPNMRYGS MFLSYSVGRK LPMKGVNWVT RDSNRLLNFS NRYSAVISDI DVKRTEEELN VALSDIRWN DHRRIYWKCS FCGSSYRKNV SVRTKYHAGC NFCKKKYPSE VRGEQHGSPP VSQAAPELTE QLVDNGKRDN L ATLAVTSK FYAEWTCRGC GGSYRSTIRS RTGQVEAGQC TLHPDIVAWS AYCPSCSWRP NMVPIAEEVS RSGQFLGLER SM EELGGQR GKQLPIPRRK RLAC

Macromolecule #49: Rhodanese domain-containing protein

• Macromolecule: Name: Rhodanese domain-containing protein / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Tb427.10
• Molecular weight: Theoretical: 30.096051 KDa

Sequence

String:

MLVSYLRLQA DRRTRHHMMV DGQINPREWF NWHPKEYQPW YFDRHKFLYE QRQYAQFHHL LCHQVYLKDM LHLMRYPEPY THIIDCRTS TQKMHRWVPH SLWLPRDEVE YALQLSADEF LDMYGFRKPD KSDDVILLSH NGIYSEQAGW EWKKQFFQHV Y NYRGGTNE LFGESYSDMN VSDVLSPWKG PFPQSGVFVD KWSKRKVLTR TGPFDRQYEM QDFALPDLEL EKARHPEEGP RQ NMPFGLQ

Macromolecule #50: Ubiquitin-like domain-containing protein

• Macromolecule: Name: Ubiquitin-like domain-containing protein / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Tb427.10
• Molecular weight: Theoretical: 26.472807 KDa

Sequence

String:

MRRGALSLLK AGLLGHYQQE AFEARKRFEE STTYPGPIRA ATPGDTRFYS GSLESILHDT DRHYWRAVTD DPRVQHLIPL RIRFKIFTW VTSGWEQRMQ VVQIMAPKDS TIAQVKDLVI VENQSPYLCV SSFHLAIDGK ELDPQKTLGE YGITEQSQID A IEQNDHLL HRDDERPRDW TVDEITAEDV KRSPYKEMEM QPLQNLAPRY EARPKGYFGR TYYSGMKQSS

Macromolecule #51: mS69

• Macromolecule: Name: mS69 / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Tb427.10
• Molecular weight: Theoretical: 21.589732 KDa

Sequence

String:

MRRVRDGLFL SHPSSALQCS VAVVSTGEFD HPPFQFRQRH TFNTTPLHDA NRFGGRTAYL REIGPVNIKK QGRRFKKDPR TVQFNVDVW CAQQTLRKRW KQRDWEVIEI PFRLVPREQQ RVIPELYTDI PQMTDPARND FSNIRNKVYD REELQGVLFP A AGAMLYPP LQRVDKQAMT LDKYL

Macromolecule #52: mS70

• Macromolecule: Name: mS70 / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Tb427.10
• Molecular weight: Theoretical: 21.247398 KDa

Sequence

String:

MLRFTHRALT ATPERFSVLG TTHPKPKRTG FGRNNKMRSK PSDNVAWYDK GPVEWLPRPV RLTYDHLDQL QQWTMRATLD GRTEEFNRI RDLHREWSQH PLMPVLGDVE PKFPLNLFKQ NHRAKKRFLV RWHKANTPAN WLWMPRGPTV VTPLHRTNPT Q YPENWKQM VQRKSGTGTP S

Macromolecule #53: mS71

• Macromolecule: Name: mS71 / type: protein_or_peptide / ID: 53 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Tb427.10
• Molecular weight: Theoretical: 20.093227 KDa

Sequence

String:

MFHRAFVSSS DLTGCTIALS SVCTQKRYWA KPKKRPKVGQ GFHEKAQKWR EEYLLDRHRA LADSLRAYVE FSTSKRVEPW DARFKPFDR IEKDGVYVLM RYMMEEKLQL CNYHHRPVKR LFCNIGLMGP QITTKARWKP YRFATNPAGT SKAERMYQRD K TVYTHGHN D

Macromolecule #54: mS72

• Macromolecule: Name: mS72 / type: protein_or_peptide / ID: 54 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Tb427.10
• Molecular weight: Theoretical: 19.340352 KDa

Sequence

String:

MLRSTLTSLN TFLTSSVATP PISVIRTGPK WWAHPERMVR QKLMYFTLGV DQLPLRRTAV IQRDLQRFHM CKPPPRVGDS TGYKRSRAA QLNTWYRRIQ YQEYHMQHLF TRHVWGLLRV YPGNTTKIQG KADDGYVGYD SVPFHRYNRA PLPFPARELY E RRK

Macromolecule #55: mS73

• Macromolecule: Name: mS73 / type: protein_or_peptide / ID: 55 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Tb427.10
• Molecular weight: Theoretical: 11.360885 KDa

Sequence

String:

MLRRSILFRM KYADLELTTR GEFPHGMKEP AFVKKLDQNI PWYFSTYRSM YHWPITGDNW SDLNEAEKHH DLHMFYTLAW WKLGEGIFG VDEDS

Macromolecule #56: mS74

• Macromolecule: Name: mS74 / type: protein_or_peptide / ID: 56 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Tb427.10
• Molecular weight: Theoretical: 8.203408 KDa

Sequence

String:

MFSLTQTWLI AHWYCGHKFR HRFMRDKRFH PSLQASHDAR NRFSKRRHFK TNRWNYQQAY RDMP

Macromolecule #57: mt-SAF3

• Macromolecule: Name: mt-SAF3 / type: protein_or_peptide / ID: 57 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Tb427.10
• Molecular weight: Theoretical: 106.621727 KDa

Sequence

String:

MLRGWHPNSS AMQVGMRHIT IGGRHSRGGF RQPLGKHPQV KQGTVEGVPR RIPGTTKVTY TNKKGRTFSF SVPVSELTHP QVTLESAAG TWREMDTSFC ELGDIEDDMP SPVDECLRGG SSLDKRLIQE VRERFVSFCR EYVLMDTSGM KSTILSTELN A GPDYEHYD RRLRRKRHWL AIRHRFEDVR YVIWPDVVEE TARGDSAQAD VSLTNPSLTA GEMLEALLWL DAASTFCVRK VH PSDLGDK SEFLPLDLQR EVEVVACHAR RDLDFFDPSA TSLEQFTACA ALCVNHRVPF SLFFPAQDVC GDASVSTGQC IVA NAPSPH TALGAVRIMA LISEGSGSDI GKTIMFSDAF GAVTRFGILR GLSRVMSVEA FGCKDALENV NESELCIILH FCAE VREQN AAFFRRYEAS EEDSDPQQVS FLAKYQQLSQ IALARCKRLL YHPDSPRAQV MSEDGYIPLV ELQRHAEGTN KAALI HYNL GIRSAQGMRR VALGAQSSAR LAELVSRLEE ASARVSGNTL VNDLVHHLSH KAAAGKMSLT LREVNTLLPL LSRMRR ESP NGALDARFDR VFNAIDTAIG AAMRHNCTLD ELLDLAEGLA ACEMVPSALK QVEMVLIRSV MMHECSPMHL RRMLQAM FT LMRTSVPQVL LQSVASRVAD YIKEASHMDS SSSNGGGDEK VKNHEECEQL LELLVVLGKC GYGALPGLVT IYWEAQLI D SMQLNPRLRC SYASLLASAA FALKKHDKRA WEGLADESHR LFMEYTRCNK ENDIGRFAEC VTGLAVLTQI KDNTNSSDV AFLKEYLSAT SLELKSCEVI RVQELTDLLG RTLEWSEALG VVAPDVVIQL EKALFVMLEN VSHTAPGVGI PDELVTAACC LVDMSSASL ELRKAAAGVV GGAIVHAEEA LETLRSGAPT QVRPGHSFDV AALASAEREN VYKNSILQYC AALQRSGMST H VEELWS

Macromolecule #58: mt-SAF6

• Macromolecule: Name: mt-SAF6 / type: protein_or_peptide / ID: 58 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Tb427.10
• Molecular weight: Theoretical: 76.781398 KDa

Sequence

String:

MYKKALHSFF LKVHPDFFHH NRSQQTVNES SVARLNELLS WAKAFKSGHL QPPPSSSFTL TFYRKPDDNM GNGETRREGS GSPVSSLVG RSMGDGSLSP TIIQSTFELP SNFAPSDNHR GTVERAVNKF LRDLLRRAAC IDSVTESISE AEDATAARAE A KPLRRRPR GSQHRGAPTG PKSLLDEAVE SMTVQWSLTP APTLQELIEA DQILFSRDLS PLQSAAALST LQRHLGELNY SA WESMPVI VSNQFSIGDL TGTITIPWDF TPEQFHSFMA HNEKGVARCR EVAIQYASTI EQLIAELCTA LELDDILVSC SHQ DALRLM ELLHRNRELL IQYGLSKLTL EVGNRHATRA NGVVIINCSL TSEQLRPWLK AISPKLPLQQ RLYELSKQML ESTL WHLKE FRTMVEPGGV DAFSNDCTYA ERLQWSKELF RIGPSLAPWD WSEMTFVLSP DVDIDWANGL LALPYNFDGD ALVRY VEEV QQEAKSRKRE ELLAASAMQR EEEERRRKQQ HDEELMVECQ EKGERSGDDS PTVAERMRHL YRQTNPHMDE YLASSN SRV DTLPVERPLS HAVTFNSDAE AEDQLKWEGF YAEPYVDQAP TSDIDDMAHT FMLTNRWHRE EAAKKMLDQL RGTYGKK SR RFEYQKMGDV LEINNAKVQP KGFPTLTRGI KPGC

Macromolecule #59: mt-SAF7

• Macromolecule: Name: mt-SAF7 / type: protein_or_peptide / ID: 59 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Tb427.10
• Molecular weight: Theoretical: 76.675156 KDa

Sequence

String:

MLSHAVPRLR LAAAAVKAAE WYAAENRRKQ KGGDVIREKV DCTAPLWFDD TEQNLLAREQ HVRSPAYREL HRPALTNAAM GLYGGEPGF EKAHRVWVDP DRPHIKHIYN QTALARNLRY ARYGYFKRDM HLLDVDKLVR HARLLPTPGR LLTDFLYQRV P LPDKSCAA LIRYQRQQIE MLEVWGRHAS FQCAVEMFER MIVTNIPPVE VGVETHGEMV LCAAACGKWE EGWNVYANRA RE LEKESPE SFILNTFFFD ALLTLCVAAG RVSEGIDTLE EVIKRNLRPR GTMLNKAMIL YSILGEQMSK HEASRATSEG ERS YLCEPE EVEKMGLEVW SLFDFYQLPR TTASIEAYMR MCCAFNKPTL VLKAQGFADA SDIRLSIECF HWLVYAIRGV AGFG DYVMD VLSQLRPRGL TPDFVLFTLS FMYCALQRDG ELALAIFDQH FVHQNMNPTP EMVLLFIQAC SNCEEPTAVM LERSE TLIK RLEAVGSSVD LISPIYDQFL ELCAHLGAVA SGFSALKRIV GFGKPLTTRM INSLLLANSN AISSNGSLSM TEELVG FFT LLKIRPNADT EICVNLCRDA FGESPVVNDF IKVIGESLQG DSEKGEAPQY DEDIPVIQVP PHELRQLRTE WKLSPRD IV LRRFGQHTKP PGKAALDVGS MRGSVIPFGR SPGEQLV

Macromolecule #60: mt-SAF9

• Macromolecule: Name: mt-SAF9 / type: protein_or_peptide / ID: 60 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Tb427.10
• Molecular weight: Theoretical: 69.781789 KDa

Sequence

String:

MTGPTRALFL SSGINLGRLR LAEQFSSMNG WQSKEDPAFD AYVKERRRKE NYEAFDQRVE RGYAAAAKLH KAEIQNAVKR RLKSSGAKF TAETLREMSS AVTERLAWLR DVWAQIDADY RSGDSARQET AAQEISAALR GEPNDYMRWV YETKRELRFA G PVGRRAIQ EELQAAELPE VLDEEVNRYH DLKLNMMEIE REVKAKYGVA GQQHWAELQA AKDEEYIQKL DEAAEVYKQL LD QSARLDE SRRSELQRSY VERVHQAQVR FKAAMELEGQ REQLIEAHQA MKEERMRTER EKRRQLLREA AELRAQGKKS ADV LTALKE RQLDANAKRQ AEYELKECED ILKRKSEMLD MIAHFKHDVE EREGREMLQR QKSDEERQVN VFGFYEEVGV EDGL SISSE GTTSQGGSSG LGTVSTSTSC AKSADSNSSA QPSQKLRKEE LWKVINADTY EDPFRTVHQA RLDAVKTYDP AYART FPLN LVLGRKYSRQ GAGEMAAGNE TDKQILQKGN NILYSFQWGL NNGTVHDLDA DGGTDYFMDG AFHVRDKETG DIDWRY EKK RGGPVFRGPK FYRLGAQREA ADPGERAMDP TPYTSTPREH KWRSS

Macromolecule #61: mt-SAF22

• Macromolecule: Name: mt-SAF22 / type: protein_or_peptide / ID: 61 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Tb427.10
• Molecular weight: Theoretical: 39.132621 KDa

Sequence

String:

MTDPVRRDPT EFLRVLRRMS RTTSWQKTML FASKGRMVGY RLTREHYNTV LFSQSLWGRA LEIVRVVRAM QEDKVQPNGA TYYYIVNGM GNADHGWNYD FRINRRLEKI QHWRVALEAL EACEANGFDS TDTMHNSALI TLVIPGFNRW QQASLLLQRM L REDRRMHP TMVKFYHDCL VRNNRPREAS SLMRLAAERG VHGYEDKWEA DVYKGRPLDS EVMNESEGNG LRRQASSLAF AS LMLRGDQ RPLPENLQAL LEEETTRNIE AERSVPVPFS AGLHATEINS VFRPRVYRQL WYKWQHIANR YRPTAALKRR QLA PRDSPT GIPGFYRI

Macromolecule #62: DNA photolyase, putative

• Macromolecule: Name: DNA photolyase, putative / type: protein_or_peptide / ID: 62 / Number of copies: 1 / Enantiomer: LEVO / EC number: deoxyribodipyrimidine photo-lyase
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Tb427.10
• Molecular weight: Theoretical: 95.285312 KDa

Sequence

String:

MKRIFRSSVR VGAALPLGAV VPAIFYCNGI RIMGRQYTHD TYRPFGDGYK EVRGTAPTDE CNLLFASLND ESLPLCEDSG NVVNGQLVR ASALVDSDAL ADSQELVDVA IEEDRREEED NSVLLVP(UNK)HE EQNSVTKKQN EGEDVTTRCD HLFSVVG CP TTVSTATSGI PNTPSRNYLS EQDALYLSPT TPEEARVPSA LCPQLSACVM VVFAANDMRV HDNYALALAA TRAEAAGG L PVIAVTVVDY RMFAQPSAVG GFFRQSPMRA RFFLETLAAL RYKLERELRI PLLVRCGRPE DHIPRLVVEC GAIDVFLTT QYAPHEKEVH NDIVEAINQR RWVSRDPTAG YKAIEHDATV VHPFGATSAS TKVGVKESRL TAAPVAHSVW QTTLVHIDDL PVPVASMVE GERWYHDDVT TANIRPTAAF DRCINRLSDL PARGDLLPTT GELSGLEPPP LYRGRAPTLV ELGYGSPEAF I EEEVIATQ SSHPPGEDAA IERVLDWLMD GGMTSMLRYG RDRRTNTKMY SQRLSRLSPY LSCGALSPRR FYEELRRFTS EH LRDNFVQ QQYREALLRL SRRDYWHWMG LRYGPLLFYE YGPRPEHTDD IADWRHDPKI VQKWCAGLTG IPFADAAMRE LTL TGFVAD EGRQGLIWLL SRGYGQDWRL GAEWLERCSI DYDPFVCYGN CAYYSELVRD DFGETVRNIH WLAHHHDQTG IYVK KWLPQ LSKIPPVYIH RPHVLTERMQ AMHGVQLGKN YPYPLKLWDG AQYTLSSEHL TTYFSEGSQW HRKAKNGAGG SGYAE ALRH GTVILEPHQL QSSAFGELEH GVMHTVPVVK DDTAGRGQHN RSLTASDE

Macromolecule #63: Acyl transferase-like protein

• Macromolecule: Name: Acyl transferase-like protein / type: protein_or_peptide / ID: 63 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Tb427.10
• Molecular weight: Theoretical: 60.722109 KDa

Sequence

String:

MSKSLQRALV FSGQGAHRRG MATEYLRIPA VACLWERMKG SMEQKYGISL QDIITENPTR LYVYNDAYDV AAICERSCQD GGSVVESTP KQKLISHPQG VLSLTSLTQP CMLAAHMVSL EYLKETRGYS VESSEVIAGH SLGEFSALCA LGVLSPEVAI D LVYRRGAL MEDAVKHSSQ SNEGHLMFAC NPQRAKLCVE DPDLAVDQLH IFVELIARNL STTASFIEVV NYNISYEQYV VV GDPIALS ALGKCLDPQF RATSCGSSAT LDNIVRTAVS SVLQDKQEGI TMNPNTGPAP DFVTSCARKY GIRSTFRRFL RGP DDGYTP SLEELTHLTL QEDGRSGLKK KSWFIPLPLS IPFHSSMLRR AMDLFLPVVR DALPDEETMR SFFCIPQCGG SD (UNK)GPSSRC ASEPKKPVWL TNLTGTAFRP FDVDFQRDAL DAMQSMNVGE IRHNGRYHTN LVELAFKNGM DTSSVRDMC AAVLAAQLAH PVQWIDVMDS AVFQHGVREA HEISPVRTVA DMFKRTVFRG KTSDASVGEA ALDIVTRCLP SEERFL

Macromolecule #64: mt-SAF37

• Macromolecule: Name: mt-SAF37 / type: protein_or_peptide / ID: 64 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Tb427.10
• Molecular weight: Theoretical: 37.00909 KDa

Sequence

String:

MWQRLRFDRL SSSVRRTNLN PLKPCAALTE QRAELRNLHQ YPTARHKSLV KDRLRFARNW WLTGGNNYEL VHEVGHEREA TECFAEYAQ DSSRDVYLMS TNRLSDLPPG DRLKAIVGLM RSRWEVKDAN RGYDKAKLLL QALECFSEMK ASGQIGDFNS L PEPDQDTF LQYVEGCSRF AQACSHSHPD AVRVLLRAAQ ICEEMRCVEK RDEMIQVTEA AANRMDRAYA FSRPHDTLRA AP PSLHENE DCVRLKNTEE LRRRFGNTAP HVLEKPKRVD CLRIHRNRPL LLHPMKDNNK LLELSKLPAR PEFDSWTSHQ T

Macromolecule #65: mt-SAF38

• Macromolecule: Name: mt-SAF38 / type: protein_or_peptide / ID: 65 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Tb427.10
• Molecular weight: Theoretical: 69.516695 KDa

Sequence

String:

MRGSLPLLFN PVLPPSTARL RLLTYPMALA QPHATVPLIQ PTIDGTHDGR NGATVSLRTQ ARMHGTADGT MATAGDSSQN NSVMDSPRW LRNPDELCVA ALRRSRDVNK INSYVATYKF DDPQWAPLLL PEVTLRPQVN STGDKPNGGN EAAADVVSVG P SVSATPES TPPPPPSSSS SSPYSCPADC VSISHNKMIM LECMSRHVNF SLRHIVQKGH GIYLIYHAQH SILQPKGLVE QS FVTCSFG IRGERLRTDI VHVGPIDAAD VMELQPSEGH DHPRCCFNLY QKSDVRRGVI AVSQVEGYGT WFQRKPMLWQ RSR RIGALQ SQLGAFAYDL VDPHEVGKWR DCEVSLLAPH MRFFRNGLNG AEAVGIIASS QVAQQRRLYL GEFEAPAITA LDAV QQLAH ASALRCKLVT PVVDPNGVGG TGSGSLGDEN MDKHIDMETL LPLSWATRTP PPYVPLEADL PFKLQMSRPT VFAES HQQN QAYPTGGTVG SPFVRGAPMM MFEYNMHQGV DHYVYDDAPS ARPMKWWSQK SNMPYSGYMY FARSGLVDRF TPSEDI PNP LEPTSKRKPL HAVVPPTKVV QERLRKYRRK QQEGHKQRRR ASSGSGVSNE PDAVNRQESV SRGTCE

Macromolecule #66: Translation initiation factor IF-2, putative

• Macromolecule: Name: Translation initiation factor IF-2, putative / type: protein_or_peptide / ID: 66 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Tb427.10
• Molecular weight: Theoretical: 87.113992 KDa

Sequence

String:

MQSGLCHCVR RVAALGSVAN LHHFTNLPSG FSVWTSRRWQ SQSSRGQPGL QWQEGVVDPR YSSSGNMRRF NPDTVAHYVK ATIQNDRRE MGLGEVYDWH EFAKDAVYIP TRSGPLWVGS DDPRCAKFMR RRSKMQKTPQ QKARPKPGPD PAKALEDHPL R EYFTTATN LRDPLSTANN LHRAGLIREY DIKYTAAKVR YVPRPPVVSI MGHVDHGKTT LLDYLRKTNV ASQEAGGITQ NV GAFQVKT LGDTLVTFID TPGHAAFTTM REVGATANDL IVLVVSAVDG VQPQTKEVIE LAHKSGIPFV VACTKIDRQP RVE NVKQQL RDCNVELEED GGDTQFVPVC ARDGRGVPEL LEAIALQAEL CEISTPKPSR CEVTVIETNG VNTTTEVAGI VRCG KVRAG QVLVAGMTYA VVRKVLDEHG QIIQEAGPSQ PVVLHGFRVH PKPGSILLQV SSESHAQKFY HFMKEVYQAE GRRED YLQL LNQEQHGMLY ARKPDNNLVR TYSTQAFVLS CKAATFGMLQ ALMKSIYEIP RLEGISTEIK VTEVGGLRDY DVALIG SSG QPGCILLYGG CKDTNTLDVP NHVTVIRFNV LYHGLEALKE TLVGALPKIT KIRVTAEAEC LQVFRASQAG KSGNAGG MR VTKGTIIAAH LTFRVCRKAP LVKGTAETDG VSDNEGDGDE DMLGHRVVVY EGQIKELRRF KELVPSVELG LECGVIMQ D EFQFRTGDIL QQYETYEEPR DVAEEYQKAE LREKIMRDTA AAEALLAEST GGEDKPTEES EVKTTI

Macromolecule #67: mt-SAF39

• Macromolecule: Name: mt-SAF39 / type: protein_or_peptide / ID: 67 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Tb427.10
• Molecular weight: Theoretical: 89.639992 KDa

Sequence

String:

MRRSGRGSAV RWSSLCKCQC CLYRTPLGGT YFEQALPRSL GARQGKGVLS TVNTALSRKA LKRRQSLPRK KLNVPLTAEG LKERLKQLS AEERELSIKN NTEECDEPSP NEFTTTHEAR VALARVLHHG ENAGERKEVA MRIPSFCRSP AVSETQSIVV D DKEGDITN AAVHVGCSVL GSDLDHLERD MIRDYHQRGK KLPTFDNIYR TLGCGRKGTS VSDTEPEDEN SSGAIQSECG LG DAGRRGT VVVAPSHLHH STPPTKGRSG EEEEEGGCFD TNTLPADANP HFPPGACDNE VLAPLSGGCA ASEQTEITDT ASF IPSNSR LSTAVYDAYR QRPADDRLVV LRGTDFWDNE ENRARLQELT DYAEEDFARE MLMEGAMDTS EVGYSTNKVR KETL LYFQA HPINEMIQEP FARVRSILPS DGGPEVHFPA DDPDTDVDIP TAQARTMARE LGLDLIRVGT LYTPINDRRV VAVCT IADH REHMRDMIRF KIKKLGVQRP PTKEGIEVPF RGGTHPHAVR FKSIGIAKHL LLGHVVRINL TDFGTVREGF PVFGSI LDE VARQALQLHA YHTAGVVRAN YNEVYCYLYP STGRSPKSTV LHPTQEQLAT VRDRCLLERE REVYFDGLYD KKTPRER LT YMRKLQDGTA WADRDDGLSL QRQRDMKVML GYLPKGNHEL YAARGDVNVP APFRASHPTS VDRWTHPQES NLEQAARG S AVLAKRLSMT VSEMHDRQET AENPATLDRF YYRIQGPALE AGELKEALGL KGNRKRLPRR APGWATLGME KVSPQEPGH AAK

Macromolecule #68: Unk

• Macromolecule: Name: Unk / type: protein_or_peptide / ID: 68 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Tb427.10
• Molecular weight: Theoretical: 1.805216 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)

Macromolecule #69: Unk7

• Macromolecule: Name: Unk7 / type: protein_or_peptide / ID: 69 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Tb427.10
• Molecular weight: Theoretical: 3.422209 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)

Macromolecule #70: UnkE

• Macromolecule: Name: UnkE / type: protein_or_peptide / ID: 70 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Tb427.10
• Molecular weight: Theoretical: 4.528573 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)

Macromolecule #71: UnkF

• Macromolecule: Name: UnkF / type: protein_or_peptide / ID: 71 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Tb427.10
• Molecular weight: Theoretical: 3.337105 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)

Macromolecule #72: UnkG

• Macromolecule: Name: UnkG / type: protein_or_peptide / ID: 72 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Tb427.10
• Molecular weight: Theoretical: 1.124378 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)

Macromolecule #73: UnkI

• Macromolecule: Name: UnkI / type: protein_or_peptide / ID: 73 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Tb427.10
• Molecular weight: Theoretical: 869.063 Da

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

Macromolecule #74: UnkK

• Macromolecule: Name: UnkK / type: protein_or_peptide / ID: 74 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Tb427.10
• Molecular weight: Theoretical: 273.33 Da

Sequence

String:

(UNK)(UNK)(UNK)

Macromolecule #75: UnkL

• Macromolecule: Name: UnkL / type: protein_or_peptide / ID: 75 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Tb427.10
• Molecular weight: Theoretical: 1.720111 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)

Macromolecule #76: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 76 / Number of copies: 6 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Macromolecule #77: ADENOSINE-5'-TRIPHOSPHATE

• Macromolecule: Name: ADENOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 77 / Number of copies: 1 / Formula: ATP
• Molecular weight: Theoretical: 507.181 Da

Chemical component information

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM

Macromolecule #78: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 78 / Number of copies: 4 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Macromolecule #79: URIDINE 5'-TRIPHOSPHATE

• Macromolecule: Name: URIDINE 5'-TRIPHOSPHATE / type: ligand / ID: 79 / Number of copies: 1 / Formula: UTP
• Molecular weight: Theoretical: 484.141 Da

Chemical component information

ChemComp-UTP:
URIDINE 5'-TRIPHOSPHATE / UTP*YM

Macromolecule #80: FLAVIN-ADENINE DINUCLEOTIDE

• Macromolecule: Name: FLAVIN-ADENINE DINUCLEOTIDE / type: ligand / ID: 80 / Number of copies: 1 / Formula: FAD
• Molecular weight: Theoretical: 785.55 Da

Chemical component information

ChemComp-FAD:
FLAVIN-ADENINE DINUCLEOTIDE / FAD*YM

Macromolecule #81: GUANOSINE-5'-DIPHOSPHATE

• Macromolecule: Name: GUANOSINE-5'-DIPHOSPHATE / type: ligand / ID: 81 / Number of copies: 1 / Formula: GDP
• Molecular weight: Theoretical: 443.201 Da

Chemical component information

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM

Macromolecule #82: PHOSPHATE ION

• Macromolecule: Name: PHOSPHATE ION / type: ligand / ID: 82 / Number of copies: 1 / Formula: PO4
• Molecular weight: Theoretical: 94.971 Da

Chemical component information

ChemComp-PO4:
PHOSPHATE ION

Macromolecule #83: water

• Macromolecule: Name: water / type: ligand / ID: 83 / Number of copies: 5 / Formula: HOH
• Molecular weight: Theoretical: 18.015 Da

Chemical component information

ChemComp-HOH:
WATER

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Buffer: pH: 7.4
• Grid: Model: Quantifoil R2/2 / Material: COPPER / Support film - Material: CARBON / Support film - topology: CONTINUOUS
• Vitrification: Cryogen name: ETHANE-PROPANE / Chamber humidity: 98 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV

Electron microscopy

• Microscope: FEI TITAN KRIOS
• Image recording: Film or detector model: FEI FALCON III (4k x 4k) / Average electron dose: 40.0 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm
• Sample stage: Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• CTF correction: Software - Name: Gctf
• Final reconstruction: Resolution.type: BY AUTHOR / Resolution: 3.7 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION / Number images used: 17391
• Initial angle assignment: Type: MAXIMUM LIKELIHOOD / Software - Name: RELION
• Final angle assignment: Type: MAXIMUM LIKELIHOOD / Software - Name: RELION