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- EMDB-13485: Cryo-EM structure of Bestrhodopsin (rhodopsin-rhodopsin-bestrophi... -
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Open data
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Basic information
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Title | Cryo-EM structure of Bestrhodopsin (rhodopsin-rhodopsin-bestrophin) complex | |||||||||
![]() | Sharpened cryoEM map | |||||||||
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Biological species | ![]() ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.21 Å | |||||||||
![]() | Matzov D / Kaczmarczyk I / Shalev-Benami M | |||||||||
Funding support | 1 items
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![]() | ![]() Title: Rhodopsin-bestrophin fusion proteins from unicellular algae form gigantic pentameric ion channels. Authors: Andrey Rozenberg / Igor Kaczmarczyk / Donna Matzov / Johannes Vierock / Takashi Nagata / Masahiro Sugiura / Kota Katayama / Yuma Kawasaki / Masae Konno / Yujiro Nagasaka / Mako Aoyama / ...Authors: Andrey Rozenberg / Igor Kaczmarczyk / Donna Matzov / Johannes Vierock / Takashi Nagata / Masahiro Sugiura / Kota Katayama / Yuma Kawasaki / Masae Konno / Yujiro Nagasaka / Mako Aoyama / Ishita Das / Efrat Pahima / Jonathan Church / Suliman Adam / Veniamin A Borin / Ariel Chazan / Sandra Augustin / Jonas Wietek / Julien Dine / Yoav Peleg / Akira Kawanabe / Yuichiro Fujiwara / Ofer Yizhar / Mordechai Sheves / Igor Schapiro / Yuji Furutani / Hideki Kandori / Keiichi Inoue / Peter Hegemann / Oded Béjà / Moran Shalev-Benami / ![]() ![]() ![]() Abstract: Many organisms sense light using rhodopsins, photoreceptive proteins containing a retinal chromophore. Here we report the discovery, structure and biophysical characterization of bestrhodopsins, a ...Many organisms sense light using rhodopsins, photoreceptive proteins containing a retinal chromophore. Here we report the discovery, structure and biophysical characterization of bestrhodopsins, a microbial rhodopsin subfamily from marine unicellular algae, in which one rhodopsin domain of eight transmembrane helices or, more often, two such domains in tandem, are C-terminally fused to a bestrophin channel. Cryo-EM analysis of a rhodopsin-rhodopsin-bestrophin fusion revealed that it forms a pentameric megacomplex (~700 kDa) with five rhodopsin pseudodimers surrounding the channel in the center. Bestrhodopsins are metastable and undergo photoconversion between red- and green-absorbing or green- and UVA-absorbing forms in the different variants. The retinal chromophore, in a unique binding pocket, photoisomerizes from all-trans to 11-cis form. Heterologously expressed bestrhodopsin behaves as a light-modulated anion channel. | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 223.5 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 19.2 KB 19.2 KB | Display Display | ![]() |
Images | ![]() | 143.5 KB | ||
Others | ![]() ![]() ![]() | 191.1 MB 194.4 MB 194.4 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
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Annotation | Sharpened cryoEM map | ||||||||||||||||||||||||||||||||||||
Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 0.86 Å | ||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Additional map: Unsharpened cryoEM map
File | emd_13485_additional_1.map | ||||||||||||
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Annotation | Unsharpened cryoEM map | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: half map 1
File | emd_13485_half_map_1.map | ||||||||||||
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Annotation | half map 1 | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: half map 2
File | emd_13485_half_map_2.map | ||||||||||||
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Annotation | half map 2 | ||||||||||||
Projections & Slices |
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Density Histograms |
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Sample components
-Entire : Rhodopsin
Entire | Name: Rhodopsin |
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Components |
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-Supramolecule #1: Rhodopsin
Supramolecule | Name: Rhodopsin / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1 |
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Source (natural) | Organism: ![]() |
Recombinant expression | Organism: ![]() ![]() |
Molecular weight | Theoretical: 670 KDa |
-Macromolecule #1: Rhodopsin
Macromolecule | Name: Rhodopsin / type: protein_or_peptide / ID: 1 / Number of copies: 5 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 134.01025 KDa |
Recombinant expression | Organism: ![]() ![]() |
Sequence | String: MASSTAAPPA AVPAIETAAP PSPEPRDSVS VNEGGDASSA QAGAAVGSTI IVGAPELEKE YSDLNFAQVA RDEGRRCLLM CVAFAIAIA HLYIYPALFG VRIVDQAEVP AEERTYFHHG WTAMLVIFFI EGVTVFLKVC STRKTRWLEK AVLQKLDGNI G VLIGEYIV ...String: MASSTAAPPA AVPAIETAAP PSPEPRDSVS VNEGGDASSA QAGAAVGSTI IVGAPELEKE YSDLNFAQVA RDEGRRCLLM CVAFAIAIA HLYIYPALFG VRIVDQAEVP AEERTYFHHG WTAMLVIFFI EGVTVFLKVC STRKTRWLEK AVLQKLDGNI G VLIGEYIV VAATYIIMGA NLIPVFEEPR SGRRVYAVRY MEWTIDACGL VYLDCRILFG MPFSKFRMLL VYSVLYMLFG LW AALASTW MWYAIFLSAS WFFFGLVCYY YWTFHRQNPS PLQQFGRAPI KQAILVFVIV WWVLYGVLFM LCFQAPDVVP QWL EQLLWT GMDVVMKLSH TVVLMAWRET QWEIDAVVDR QKVEAGRAIA QLDHQRAIHE RDLVRLRSRV YYGEHIKSEE EIKS EEVIS RSLRARKSRQ GQDGTEPSSA LSGASSTKEP PALEENSGSA APSWTAVLAK GKATSSPFAR VNKIFMREAG LCLVL CLAF VVALLHLPVY SEWFGVEVLD AEAVPHDELG FFHHGWTTML VVFLIESITV LLKVWSTWHD PRLAENVAQQ LSGNLG VLI AEYLVVGATY VILGYNLMPV FVVHRPGVAS RRVYAVRYME WAVDATGLIW LDCHCLFSRN FNEFRMAIVW TVAYMLF GL WSALASTWAW YWAFLLASWA AFLIVCLILV RFLRQDPYPH QPFGKTSVKP CILAFIIGWW VLYGILFMVC FQAPDAVP Q WLEQFLWTGM DVVMKLSHTV VLMAWRTTEW NVCELHGRNQ AEKSKKSLLN EGSHARQEKQ QLEAIMLEGG GVEAAGRVG SVSFAPSSSR RRKESDSTNW TATPGLRVDL SSMVRLEGQL AQGLVTDVHR KGMMRSEDLA ELKRLEESGF LQAQQHRNWE SQTREMTFL AHGINHIAYD PRSWMKTLTA VRGRAPTSFL LWVVLIESSI VLALSKFFGE SFDLGVSSGI HSLFGVLVSF L VVFRTQAA FKKWWSGRSA VSSLVQMSRT FAQQVCAYVK DEAYVNRMVR YSIATVVATR CHLRNTRIDP AMLLGVLKEE EI EELNRQK NLPFYTAWVI RSTLAEAVAE GACLPLHMAI ENAIKAIEQS IADAERLLTP MPFTYVVHVR TFLFIYLMGL PFI LVEDLG WLMLVAVSFL GYLMIGLENT AVQLENPFGT DCNHHPLDLY CLEVSQDLLH LLDLRASAKA Q |
-Macromolecule #2: RETINAL
Macromolecule | Name: RETINAL / type: ligand / ID: 2 / Number of copies: 5 / Formula: RET |
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Molecular weight | Theoretical: 284.436 Da |
Chemical component information | ![]() ChemComp-RET: |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 7.5 |
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Grid | Model: Quantifoil R1.2/1.3 / Material: GOLD / Mesh: 400 / Pretreatment - Type: GLOW DISCHARGE |
Vitrification | Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 295 K / Instrument: FEI VITROBOT MARK IV |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 0.86 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 3.21 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 27749 |
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Initial angle assignment | Type: MAXIMUM LIKELIHOOD |
Final angle assignment | Type: MAXIMUM LIKELIHOOD |