ジャーナル: Proc Natl Acad Sci U S A / 年: 2007 タイトル: Asymmetric binding of transferrin receptor to parvovirus capsids. 著者: Susan Hafenstein / Laura M Palermo / Victor A Kostyuchenko / Chuan Xiao / Marc C Morais / Christian D S Nelson / Valorie D Bowman / Anthony J Battisti / Paul R Chipman / Colin R Parrish / Michael G Rossmann / 要旨: Although many viruses are icosahedral when they initially bind to one or more receptor molecules on the cell surface, such an interaction is asymmetric, probably causing a breakdown in the symmetry ...Although many viruses are icosahedral when they initially bind to one or more receptor molecules on the cell surface, such an interaction is asymmetric, probably causing a breakdown in the symmetry and conformation of the original infecting virion in preparation for membrane penetration and release of the viral genome. Cryoelectron microscopy and biochemical analyses show that transferrin receptor, the cellular receptor for canine parvovirus, can bind to only one or a few of the 60 icosahedrally equivalent sites on the virion, indicating that either canine parvovirus has inherent asymmetry or binding of receptor induces asymmetry. The asymmetry of receptor binding to canine parvovirus is reminiscent of the special portal in tailed bacteriophages and some large, icosahedral viruses. Asymmetric interactions of icosahedral viruses with their hosts might be a more common phenomenon than previously thought and may have been obscured by averaging in previous crystallographic and electron microscopic structure determinations.
ダウンロード / ファイル: emd_1288.map.gz / 形式: CCP4 / 大きさ: 62.5 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
注釈
Three-dimensional reconstruction of canine parvovirus with bound feline transferrin receptor.
ボクセルのサイズ
X=Y=Z: 2.6 Å
密度
表面レベル
1: 1.5 / ムービー #1: 1.9854584
最小 - 最大
-3.0484 - 9.01338
平均 (標準偏差)
0.000000000238234 (±1.0)
対称性
空間群: 1
詳細
EMDB XML:
マップ形状
Axis order
X
Y
Z
Origin
-128
-128
-128
サイズ
256
256
256
Spacing
256
256
256
セル
A=B=C: 665.6 Å α=β=γ: 90 °
CCP4マップ ヘッダ情報:
mode
Image stored as Reals
Å/pix. X/Y/Z
2.6
2.6
2.6
M x/y/z
256
256
256
origin x/y/z
0.000
0.000
0.000
length x/y/z
665.600
665.600
665.600
α/β/γ
90.000
90.000
90.000
start NX/NY/NZ
-184
-184
-183
NX/NY/NZ
368
368
368
MAP C/R/S
1
2
3
start NC/NR/NS
-128
-128
-128
NC/NR/NS
256
256
256
D min/max/mean
-3.048
9.013
0.000
-
添付データ
-
試料の構成要素
-
全体 : Canine parvovirus complexed with feline transferrin receptor
全体
名称: Canine parvovirus complexed with feline transferrin receptor
要素
試料: Canine parvovirus complexed with feline transferrin receptor
ウイルス: Canine parvovirus 2 (ウイルス)
タンパク質・ペプチド: feline transferrin receptor
-
超分子 #1000: Canine parvovirus complexed with feline transferrin receptor
超分子
名称: Canine parvovirus complexed with feline transferrin receptor タイプ: sample / ID: 1000 集合状態: one homodimer of fTfR binds to one icosahedral CPV particle Number unique components: 2
試料ホルダー: 626 Single Tilt Cryotransfer System / 試料ホルダーモデル: GATAN LIQUID NITROGEN
-
画像解析
CTF補正
詳細: CTF correction of each particle
最終 再構成
想定した対称性 - 点群: I (正20面体型対称) / アルゴリズム: OTHER / 解像度のタイプ: BY AUTHOR / 解像度: 27.0 Å / 解像度の算出法: FSC 0.5 CUT-OFF / ソフトウェア - 名称: XMIPP with modifications 詳細: Intermediate reconstructions were made from images with virus density subtracted, final reconstruction was calculated from complete images. 使用した粒子像数: 8566
最終 角度割当
詳細: initial angles were selected using SPIDER VO EA procedure with limits for theta of 0-40, phi of 0-72 to cover an asymmetric unit of an icosahedron; During orientation search the angles were ...詳細: initial angles were selected using SPIDER VO EA procedure with limits for theta of 0-40, phi of 0-72 to cover an asymmetric unit of an icosahedron; During orientation search the angles were changed to be one of the 60 icosahedral symmetry equivalent triplet.