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- EMDB-12718: The structure of the native CNGA1/CNGB1 CNG channel from retinal rods -
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基本情報
登録情報 | データベース: EMDB / ID: EMD-12718 | |||||||||
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タイトル | The structure of the native CNGA1/CNGB1 CNG channel from retinal rods | |||||||||
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![]() | CNG channel / CNGA1 / CNGB1 / rod / MEMBRANE PROTEIN | |||||||||
機能・相同性 | ![]() intracellular cyclic nucleotide activated cation channel complex / intracellularly cGMP-activated cation channel activity / Inactivation, recovery and regulation of the phototransduction cascade / intracellularly cAMP-activated cation channel activity / Activation of the phototransduction cascade / molecular sequestering activity / response to stimulus / retina homeostasis / photoreceptor outer segment membrane / sodium ion transport ...intracellular cyclic nucleotide activated cation channel complex / intracellularly cGMP-activated cation channel activity / Inactivation, recovery and regulation of the phototransduction cascade / intracellularly cAMP-activated cation channel activity / Activation of the phototransduction cascade / molecular sequestering activity / response to stimulus / retina homeostasis / photoreceptor outer segment membrane / sodium ion transport / monoatomic cation transmembrane transport / monoatomic cation transport / photoreceptor outer segment / cGMP binding / transmembrane transporter complex / cAMP binding / visual perception / calcium ion transport / sensory perception of smell / molecular adaptor activity / positive regulation of gene expression / protein-containing complex binding / protein homodimerization activity / protein-containing complex / identical protein binding / plasma membrane / cytoplasm 類似検索 - 分子機能 | |||||||||
生物種 | ![]() ![]() | |||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.4 Å | |||||||||
![]() | Barret DCA / Marino J | |||||||||
資金援助 | ![]()
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![]() | ![]() タイトル: The structure of the native CNGA1/CNGB1 CNG channel from bovine retinal rods. 著者: Diane C A Barret / Gebhard F X Schertler / U Benjamin Kaupp / Jacopo Marino / ![]() ![]() 要旨: In rod photoreceptors of the retina, the cyclic nucleotide-gated (CNG) channel is composed of three CNGA and one CNGB subunits, and it closes in response to light activation to generate an electrical ...In rod photoreceptors of the retina, the cyclic nucleotide-gated (CNG) channel is composed of three CNGA and one CNGB subunits, and it closes in response to light activation to generate an electrical signal that is conveyed to the brain. Here we report the cryo-EM structure of the closed state of the native rod CNG channel isolated from bovine retina. The structure reveals differences between CNGA1 and CNGB1 subunits. Three CNGA1 subunits are tethered at their C terminus by a coiled-coil region. The C-helix in the cyclic nucleotide-binding domain of CNGB1 features a different orientation from that in the three CNGA1 subunits. The arginine residue R994 of CNGB1 reaches into the ionic pathway and blocks the pore, thus introducing an additional gate, which is different from the central hydrophobic gate known from homomeric CNGA channels. These results address the long-standing question of how CNGB1 subunits contribute to the function of CNG channels in visual and olfactory neurons. | |||||||||
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「今月の分子」の関連する項目 |
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マップ
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ボクセルのサイズ | X=Y=Z: 1.08 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
CCP4マップ ヘッダ情報:
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-添付データ
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試料の構成要素
-全体 : Native CNGA1/CNGB1a channel
全体 | 名称: Native CNGA1/CNGB1a channel |
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要素 |
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-超分子 #1: Native CNGA1/CNGB1a channel
超分子 | 名称: Native CNGA1/CNGB1a channel / タイプ: complex / ID: 1 / 親要素: 0 / 含まれる分子: all / 詳細: purified from rod outer segments |
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由来(天然) | 生物種: ![]() ![]() |
分子量 | 理論値: 356 KDa |
-分子 #1: cGMP-gated cation channel alpha-1
分子 | 名称: cGMP-gated cation channel alpha-1 / タイプ: protein_or_peptide / ID: 1 / コピー数: 3 / 光学異性体: LEVO |
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由来(天然) | 生物種: ![]() ![]() |
分子量 | 理論値: 79.712164 KDa |
配列 | 文字列: MKKVIINTWH SFVNIPNVVG PDVEKEITRM ENGACSSFSG DDDDSASMFE ESETENPHAR DSFRSNTHGS GQPSQREQYL PGAIALFNV NNSSNKEQEP KEKKKKKKEK KSKPDDKNEN KKDPEKKKKK EKDKDKKKKE EKGKDKKEEE KKEVVVIDPS G NTYYNWLF ...文字列: MKKVIINTWH SFVNIPNVVG PDVEKEITRM ENGACSSFSG DDDDSASMFE ESETENPHAR DSFRSNTHGS GQPSQREQYL PGAIALFNV NNSSNKEQEP KEKKKKKKEK KSKPDDKNEN KKDPEKKKKK EKDKDKKKKE EKGKDKKEEE KKEVVVIDPS G NTYYNWLF CITLPVMYNW TMIIARACFD ELQSDYLEYW LAFDYLSDVV YLLDMFVRTR TGYLEQGLLV KEERKLIDKY KS TFQFKLD VLSVIPTDLL YIKFGWNYPE IRLNRLLRIS RMFEFFQRTE TRTNYPNIFR ISNLVMYIII IIHWNACVYF SIS KAIGFG NDTWVYPDVN DPDFGRLARK YVYSLYWSTL TLTTIGETPP PVRDSEYFFV VADFLIGVLI FATIVGNIGS MISN MNAAR AEFQARIDAI KQYMHFRNVS KDMEKRVIKW FDYLWTNKKT VDEREVLKYL PDKLRAEIAI NVHLDTLKKV RIFAD CEAG LLVELVLKLQ PQVYSPGDYI CKKGDIGREM YIIKEGKLAV VADDGITQFV VLSDGSYFGE ISILNIKGSK AGNRRT ANI KSIGYSDLFC LSKDDLMEAL TEYPDAKGML EEKGKQILMK DGLLDINIAN AGSDPKDLEE KVTRMESSVD LLQTRFA RI LAEYESMQQK LKQRLTKVEK FLKPLIDTEF SAIEGSGTES GPTDSTQD UniProtKB: Cyclic nucleotide-gated channel alpha-1 |
-分子 #2: Cyclic nucleotide-gated cation channel beta-1
分子 | 名称: Cyclic nucleotide-gated cation channel beta-1 / タイプ: protein_or_peptide / ID: 2 / コピー数: 1 / 光学異性体: LEVO |
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由来(天然) | 生物種: ![]() ![]() |
分子量 | 理論値: 155.228562 KDa |
配列 | 文字列: MLGWVQRVLP QPPGTPQKTK QEEEGTEPEP ELEPKPETAP EETELEEVSL PPEEPCVGKE VAAVTLGPQG TQETALTPPT SLQAQVSVA PEAHSSPRGW VLTWLRKGVE KVVPQPAHSS RPSQNIAAGL ESPDQQAGAQ ILGQCGTGGS DEPSEPSRAE D PGPGPWLL ...文字列: MLGWVQRVLP QPPGTPQKTK QEEEGTEPEP ELEPKPETAP EETELEEVSL PPEEPCVGKE VAAVTLGPQG TQETALTPPT SLQAQVSVA PEAHSSPRGW VLTWLRKGVE KVVPQPAHSS RPSQNIAAGL ESPDQQAGAQ ILGQCGTGGS DEPSEPSRAE D PGPGPWLL RWFEQNLEKM LPQPPKISEG WRDEPTDAAL GPEPPGPALE IKPMLQAQES PSLPAPGPPE PEEEPIPEPQ PT IQASSLP PPQDSARLMA WILHRLEMAL PQPVIRGKGG EQESDAPVTC DVQTISILPG EQEESHLILE EVDPHWEEDE HQE GSTSTS PRTSEAAPAD EEKGKVVEQT PRELPRIQEE KEDEEEEKED GEEEEEEGRE KEEEEGEEKE EEEGREKEEE EGEK KEEEG REKEEEEGGE KEDEEGREKE EEEGRGKEEE EGGEKEEEEG RGKEEVEGRE EEEDEEEEQD HSVLLDSYLV PQSEE DRSE ESETQDQSEV GGAQAQGEVG GAQALSEESE TQDQSEVGGA QDQSEVGGAQ AQGEVGGAQE QDGVGGAQDQ STSHQE LQE EALADSSGVP ATEEHPELQV EDADADSRPL IAEENPPSPV QLPLSPAKSD TLAVPGSATG SLRKRLPSQD DEAEELK ML SPAASPVVAW SDPTSPQGTD DQDRATSTAS QNSAIINDRL QELVKLFKER TEKVKEKLID PDVTSDEESP KPSPAKKA P EPAPEVKPAE AGQVEEEHYC EMLCCKFKRR PWKKYQFPQS IDPLTNLMYI LWLFFVVLAW NWNCWLIPVR WAFPYQTPD NIHLWLLMDY LCDLIYLLDI TVFQMRLQFV RGGDIITDKK EMRNNYVKSQ RFKMDMLCLL PLDLLYLKFG VNPLLRLPRC LKYMAFFEF NNRLESILSK AYVYRVIRTT AYLLYSLHLN SCLYYWASAY EGLGSTHWVY DGVGNSYIRC YYWAVKTLIT I GGLPDPRT LFEIVFQGLN YFTGVFAFSV MIGQMRDVVG AATAGQTYYR SCMDSTVKYM NFYKIPRSVQ NRVKTWYEYT WH SQGMLDE SELMVQLPDK MRLDLAIDVN YSIVSKVALF QGCDRQMIFD MLKRLRSVVY LPNDYVCKKG EIGREMYIIQ AGQ VQVLGG PDGKSVLVTL KAGSVFGEIS LLAVGGGNRR TANVVAHGFT NLFILDKKDL NEILVHYPES QKLLRKKARR MLRN NNKPK EKSVLILPPR AGTPKLFNAA LAAAGKMGAK GGRGGRLALL RARLKELAAL EAAARQQQLL EQAKSSEDAA VGEEG SASP EQPPRPEPPA PEAPAPEPTA PEPLAPEAPA PEAPAPSSPP PASQERPEGD KDAARPEEHP VRIHVTLGPD PSEQIL LVE VPEKQEEKEK KEEETEEKEE GEEARKEKEE E UniProtKB: Cyclic nucleotide-gated channel beta-1 |
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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![]() | 単粒子再構成法 |
試料の集合状態 | particle |
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試料調製
濃度 | 0.3 mg/mL |
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緩衝液 | pH: 7.5 |
凍結 | 凍結剤: ETHANE |
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電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
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撮影 | フィルム・検出器のモデル: GATAN K3 BIOQUANTUM (6k x 4k) 平均電子線量: 1.5 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: ![]() |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD |
実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |
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画像解析
初期モデル | モデルのタイプ: EMDB MAP |
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最終 再構成 | 解像度のタイプ: BY AUTHOR / 解像度: 3.4 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 使用した粒子像数: 118084 |
初期 角度割当 | タイプ: MAXIMUM LIKELIHOOD |
最終 角度割当 | タイプ: OTHER |