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Open data
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Basic information
Entry | Database: EMDB / ID: EMD-11236 | |||||||||
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Title | Reconstruction of Tula virus surface glycoprotein lattice | |||||||||
![]() | Reconstruction of Tula virus glycoprotein spike lattice. | |||||||||
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Function / homology | ![]() symbiont-mediated suppression of host TRAF-mediated signal transduction / host cell Golgi membrane / host cell mitochondrion / symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of MAVS activity / endocytosis involved in viral entry into host cell / host cell surface / host cell endoplasmic reticulum membrane / induction by virus of host autophagy / virus-mediated perturbation of host defense response / fusion of virus membrane with host endosome membrane ...symbiont-mediated suppression of host TRAF-mediated signal transduction / host cell Golgi membrane / host cell mitochondrion / symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of MAVS activity / endocytosis involved in viral entry into host cell / host cell surface / host cell endoplasmic reticulum membrane / induction by virus of host autophagy / virus-mediated perturbation of host defense response / fusion of virus membrane with host endosome membrane / viral envelope / virion attachment to host cell / virion membrane / signal transduction / membrane / metal ion binding Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | subtomogram averaging / cryo EM / Resolution: 11.4 Å | |||||||||
![]() | Stass R / Li S / Huiskonen JT | |||||||||
Funding support | ![]()
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![]() | ![]() Title: The Hantavirus Surface Glycoprotein Lattice and Its Fusion Control Mechanism. Authors: Alexandra Serris / Robert Stass / Eduardo A Bignon / Nicolás A Muena / Jean-Claude Manuguerra / Rohit K Jangra / Sai Li / Kartik Chandran / Nicole D Tischler / Juha T Huiskonen / Felix A ...Authors: Alexandra Serris / Robert Stass / Eduardo A Bignon / Nicolás A Muena / Jean-Claude Manuguerra / Rohit K Jangra / Sai Li / Kartik Chandran / Nicole D Tischler / Juha T Huiskonen / Felix A Rey / Pablo Guardado-Calvo / ![]() ![]() ![]() ![]() ![]() ![]() Abstract: Hantaviruses are rodent-borne viruses causing serious zoonotic outbreaks worldwide for which no treatment is available. Hantavirus particles are pleomorphic and display a characteristic square ...Hantaviruses are rodent-borne viruses causing serious zoonotic outbreaks worldwide for which no treatment is available. Hantavirus particles are pleomorphic and display a characteristic square surface lattice. The envelope glycoproteins Gn and Gc form heterodimers that further assemble into tetrameric spikes, the lattice building blocks. The glycoproteins, which are the sole targets of neutralizing antibodies, drive virus entry via receptor-mediated endocytosis and endosomal membrane fusion. Here we describe the high-resolution X-ray structures of the heterodimer of Gc and the Gn head and of the homotetrameric Gn base. Docking them into an 11.4-Å-resolution cryoelectron tomography map of the hantavirus surface accounted for the complete extramembrane portion of the viral glycoprotein shell and allowed a detailed description of the surface organization of these pleomorphic virions. Our results, which further revealed a built-in mechanism controlling Gc membrane insertion for fusion, pave the way for immunogen design to protect against pathogenic hantaviruses. | |||||||||
History |
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Structure visualization
Movie |
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Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 4.4 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 17.6 KB 17.6 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 4.4 KB | Display | ![]() |
Images | ![]() | 101.2 KB | ||
Masks | ![]() | 4.8 MB | ![]() | |
Others | ![]() ![]() | 3.8 MB 3.8 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6zjmMC ![]() 4867C ![]() 6y5fC ![]() 6y5wC ![]() 6y62C ![]() 6y68C ![]() 6y6pC ![]() 6y6qC ![]() 6yrbC ![]() 6yrqC M: atomic model generated by this map C: citing same article ( |
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Similar structure data |
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Links
EMDB pages | ![]() ![]() |
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Map
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Annotation | Reconstruction of Tula virus glycoprotein spike lattice. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 4 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
-Mask #1
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Projections & Slices |
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Density Histograms |
-Half map: Half map (odd) used to estimate resolution by FSC.
File | emd_11236_half_map_1.map | ||||||||||||
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Annotation | Half map (odd) used to estimate resolution by FSC. | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: Half map (even) used to estimate resolution by FSC.
File | emd_11236_half_map_2.map | ||||||||||||
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Annotation | Half map (even) used to estimate resolution by FSC. | ||||||||||||
Projections & Slices |
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Density Histograms |
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Sample components
-Entire : Tula orthohantavirus
Entire | Name: ![]() |
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Components |
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-Supramolecule #1: Tula orthohantavirus
Supramolecule | Name: Tula orthohantavirus / type: virus / ID: 1 / Parent: 0 / Macromolecule list: #1 / NCBI-ID: 1980494 / Sci species name: Tula orthohantavirus / Virus type: VIRION / Virus isolate: STRAIN / Virus enveloped: Yes / Virus empty: No |
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Host system | Organism: ![]() ![]() |
-Macromolecule #1: Envelope polyprotein,Envelope polyprotein,Envelope polyprotein,En...
Macromolecule | Name: Envelope polyprotein,Envelope polyprotein,Envelope polyprotein,Envelope polyprotein,Envelope polyprotein,Envelope polyprotein,Envelope polyprotein,Envelope polyprotein,Envelope polyprotein type: protein_or_peptide / ID: 1 / Number of copies: 8 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 112.691258 KDa |
Recombinant expression | Organism: ![]() ![]() |
Sequence | String: KTIYELKMEC PHTVGLGQGY IIGSTELGLI SIEAASDIKL ESSCNFDLHT TSMAQKSFTQ VEWRKKSDTT DTTNAASTTF EAQTKTVNL RGTCILAPEL YDTLKKVKKT VLCYDLTCNQ THCQPTVYLI APVLTCMSIR SCMASVFTSR IQVIYEKTHC V TGQLIEGQ ...String: KTIYELKMEC PHTVGLGQGY IIGSTELGLI SIEAASDIKL ESSCNFDLHT TSMAQKSFTQ VEWRKKSDTT DTTNAASTTF EAQTKTVNL RGTCILAPEL YDTLKKVKKT VLCYDLTCNQ THCQPTVYLI APVLTCMSIR SCMASVFTSR IQVIYEKTHC V TGQLIEGQ CFNPAHTLTL SQPAHTYDTV TLPISCFFTP KKSEQLKVIK TFEGILTKTG CTENALQGYY VCFLGSHSEP LI VPSLEDI RSAEVVSRML VHPRGEDHDA IQNSQSHLRI VGPITAKVPS TSSTDTLKGT AFAGVPMYSS LSTLVRNADP EFV FSPGIV PESNHSTCDK KTVPITWTGY LPISGEMEGG SGLVPRGSGG GSGGGSWSHP QFEKGGGTGG GTLVPRGSGT GGET PLMES GWSDTAHGVG EIPMKTDLEL DFSLPSSSSY SYRRKLTNPA NKEESIPFHF QMEKQVIHAE IQPLGHWMDA TFNIK TAFH CYGACQKYSY PWQTSKCFFE KDYQYETGWG CNPGDCPGVG TGCTACGVYL DKLKSVGKAY KIISLKYTRK VCIQLG TEQ TCKHIDANDC LVTPSVKVCI VGTVSKLQPS DTLLFLGPLE QGGIILKQWC TTSCAFGDPG DIMSTPSGMR CPEHTGS FR KICGFATTPV CEYQGNTISG YKRMMATKDS FQSFNLTEPH ITTNKLEWID PDGNTRDFVN LVLNRDVSFQ DLSDNPCK V DLHTQAIEGA WGSGVGFTLT CTVGLTECPS FMTSIKACDL AMCYGSTVTN LARGSNTVKV VGKGGHSGSS FKCCHDTDC SSEGLLASAP HLERVTGFNQ IDSDKVYDDG APPCTFKCWF TKSGEWLLGI LNGNGPFEDD DDKAGWSHPQ FEKGGGSGGG SGGGSWSHP QFEKKVTGCT VFCTLAGPGA SCEAYSENGI FNISSPTCLV NKVQRFRGSE QKINFICQRV DQDVVVYCNG Q KKVILTKT LVIGQCIYTF TSLFSLMPDV AHSLAVELCV PGLHGGPFED DDDKAGWSHP QFEKGGGSGG GSGGGSWSHP QF EK |
-Macromolecule #4: 2-acetamido-2-deoxy-beta-D-glucopyranose
Macromolecule | Name: 2-acetamido-2-deoxy-beta-D-glucopyranose / type: ligand / ID: 4 / Number of copies: 6 / Formula: NAG |
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Molecular weight | Theoretical: 221.208 Da |
Chemical component information | ![]() ChemComp-NAG: |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | subtomogram averaging |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 7 |
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Vitrification | Cryogen name: ETHANE-PROPANE |
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Electron microscopy
Microscope | FEI POLARA 300 |
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Specialist optics | Energy filter - Name: GIF Quantum LS / Energy filter - Slit width: 20 eV |
Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: COUNTING / Average electron dose: 4.71 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD |
Experimental equipment | ![]() Model: Tecnai Polara / Image courtesy: FEI Company |