- EMDB-0193: Klebsiella pneumoniae type II secretion system outer membrane com... -
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Basic information
Entry
Database: EMDB / ID: EMD-0193
Title
Klebsiella pneumoniae type II secretion system outer membrane complex. PulD, PulS and PulC HR domain.
Map data
Masked sharpened map
Sample
Complex: Klebsiella pneumoniae type II secretion system outer membrane complex. PulD, PulS and PulC HR domain.
Protein or peptide: Type II secretion system protein D
Protein or peptide: Pullulanase
Protein or peptide: Pectic enzymes secretion protein OutC
Function / homology
Function and homology information
protein secretion by the type II secretion system / type II protein secretion system complex / cell outer membrane / intracellular protein transport / membrane => GO:0016020 / plasma membrane Similarity search - Function
Type II secretion system protein GspC / Chaperone lipoprotein, PulS/OutS / Chaperone lipoprotein, PulS/OutS-like / PulS/OutS-like superfamily / Type II secretion system pilotin lipoprotein (PulS_OutS) / Bacterial type II secretion system protein C signature. / Type II secretion system protein GspC, N-terminal / Type II secretion system protein C / Type II secretion system protein GspD / GspD/PilQ family ...Type II secretion system protein GspC / Chaperone lipoprotein, PulS/OutS / Chaperone lipoprotein, PulS/OutS-like / PulS/OutS-like superfamily / Type II secretion system pilotin lipoprotein (PulS_OutS) / Bacterial type II secretion system protein C signature. / Type II secretion system protein GspC, N-terminal / Type II secretion system protein C / Type II secretion system protein GspD / GspD/PilQ family / Bacterial type II secretion system protein D signature. / Type II secretion system protein GspD, conserved site / NolW-like / Bacterial type II/III secretion system short domain / NolW-like superfamily / Type II/III secretion system / Bacterial type II and III secretion system protein / PDZ superfamily Similarity search - Domain/homology
General secretion pathway protein C / Pullulanase D protein / Pullulanase-specific type II secretion system component C / Pullulanase secretion protein PulS / General secretion pathway protein D Similarity search - Component
Biological species
Klebsiella pneumoniae (bacteria)
Method
single particle reconstruction / cryo EM / Resolution: 4.3 Å
Journal: Nat Commun / Year: 2019 Title: Core architecture of a bacterial type II secretion system. Authors: Anastasia A Chernyatina / Harry H Low / Abstract: Bacterial type II secretion systems (T2SSs) translocate virulence factors, toxins and enzymes across the cell outer membrane. Here we use negative stain and cryo-electron microscopy to reveal the ...Bacterial type II secretion systems (T2SSs) translocate virulence factors, toxins and enzymes across the cell outer membrane. Here we use negative stain and cryo-electron microscopy to reveal the core architecture of an assembled T2SS from the pathogen Klebsiella pneumoniae. We show that 7 proteins form a ~2.4 MDa complex that spans the cell envelope. The outer membrane complex includes the secretin PulD, with all domains modelled, and the pilotin PulS. The inner membrane assembly platform components PulC, PulE, PulL, PulM and PulN have a relative stoichiometric ratio of 2:1:1:1:1. The PulE ATPase, PulL and PulM combine to form a flexible hexameric hub. Symmetry mismatch between the outer membrane complex and assembly platform is overcome by PulC linkers spanning the periplasm, with PulC HR domains binding independently at the secretin base. Our results show that the T2SS has a highly dynamic modular architecture, with implication for pseudo-pilus assembly and substrate loading.
History
Deposition
Aug 15, 2018
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Header (metadata) release
Aug 22, 2018
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Map release
Nov 27, 2019
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Update
Nov 25, 2020
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Current status
Nov 25, 2020
Processing site: PDBe / Status: Released
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Structure visualization
Movie
Surface view with section colored by density value
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