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-Structure paper
| タイトル | Visualization of the complete preprimosome reveals the structural mechanisms governing DNA replication restart. |
|---|---|
| ジャーナル・号・ページ | Nat Commun, Year 2026 |
| 掲載日 | 2026年5月16日 |
著者 | Peter L Ducos / Alexander T Duckworth / Kenneth A Satyshur / James L Keck / Timothy Grant / ![]() |
| PubMed 要旨 | Replication restart pathways reinitiate DNA replication processes following their premature termination. In Escherichia coli, this essential process begins with regulated assembly of the preprimosome ...Replication restart pathways reinitiate DNA replication processes following their premature termination. In Escherichia coli, this essential process begins with regulated assembly of the preprimosome complex, comprising the PriA, PriB, and DnaT proteins, onto an abandoned replication fork. Here, we present two distinct preprimosome structures. One represents an intermediate stage in preprimosome assembly with a single DnaT C-terminal domain (DnaT) bound to PriA/PriB/DNA. The second captures the mature preprimosome, in which filamentation of multiple DnaT molecules catalyzes the handoff of the single-stranded lagging-strand DNA from PriB to DnaT. The DnaT N-terminal domain forms a separate, independent oligomer in the mature structure. Taken together, our results detail the molecular mechanisms underlying replication restart initiation and regulation and suggest mechanistic similarities between DnaT and the canonical initiator protein DnaA. |
リンク | Nat Commun / PubMed:42140935 |
| 手法 | EM (単粒子) |
| 解像度 | 3.22 - 3.6 Å |
| 構造データ | EMDB-74008, PDB-9zbt: EMDB-74009, PDB-9zbu: |
| 化合物 | ![]() ChemComp-ZN: |
| 由来 |
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キーワード | REPLICATION / DNA replication / helicase / oligomer / DNA repair |
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