EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural mechanism of mRNA decoding by mammalian GTPase GTPBP1.
ジャーナル・号・ページ	Nat Commun, Vol. 17, Issue 1, Page 148, Year 2025
掲載日	2025年12月5日
著者	Denis Susorov / Anna Miścicka / Dmitrij Golovenko / Anna B Loveland / Alexandra Zinoviev / Tatyana V Pestova / Andrei A Korostelev /
PubMed 要旨	GTP-binding protein 1 (GTPBP1) is a widespread translational GTPase closely related to elongation factor eEF1A. The loss of GTPBP1 leads to neurodevelopmental and neurodegenerative disorders in ...GTP-binding protein 1 (GTPBP1) is a widespread translational GTPase closely related to elongation factor eEF1A. The loss of GTPBP1 leads to neurodevelopmental and neurodegenerative disorders in animals. Although linked to translation and quality control mechanisms, GTPBP1 molecular functions remain largely obscure. Similarly to eEF1A, GTPBP1 delivers aminoacyl-tRNA to the ribosome, but the ensuing GTPBP1-mediated elongation is slow. Here, using cryo-EM of mammalian 80S ribosomal complexes bound to GTPBP1 and aa-tRNA with GTP or the non-hydrolysable analog GDPCP, we show that the distinct GTPBP1 architecture and interactions with tRNA underlie slow GTPBP1 dissociation after GTP hydrolysis, resulting in delayed tRNA accommodation. Slow dissociation correlates with an extended proofreading stage and higher accuracy of GTPBP1-mediated decoding, potentially allowing GTPBP1 to elicit its putative quality control functions. GTPBP1 visualization provides the foundation for mapping and elucidating GTPBP1 mutations associated with human diseases.
リンク	Nat Commun / PubMed:41350250 / PubMed Central
手法	EM (単粒子)
解像度	2.9 - 3.1 Å
構造データ	73297 9ypg EMDB-73297, PDB-9ypg: GTPBP1GCPPhe-tRNAribosome in the GTPase activation-like state, Structure III* 手法: EM (単粒子) / 解像度: 3.0 Å 73302 9ypo EMDB-73302, PDB-9ypo: GTPBP1GCPPhe-tRNAribosome in the open state, Structure IIa* 手法: EM (単粒子) / 解像度: 3.0 Å 73307 9yps EMDB-73307, PDB-9yps: GTPBP1GCPPhe-tRNAribosome in the open state, Structure IIb* 手法: EM (単粒子) / 解像度: 3.0 Å 73308 9ypt EMDB-73308, PDB-9ypt: GTPBP1GCPPhe-tRNAribosome in the open state, Structure IIc* 手法: EM (単粒子) / 解像度: 3.1 Å 73310 9ypv EMDB-73310, PDB-9ypv: GTPBP1GCPPhe-tRNAribosome in the open state, Structure IId* 手法: EM (単粒子) / 解像度: 3.0 Å 73311 9ypw EMDB-73311, PDB-9ypw: GTPBP1GDPPhe-tRNAribosome in the post-GTP hydrolysis state, Structure IV 手法: EM (単粒子) / 解像度: 2.9 Å 73314 9ypy EMDB-73314, PDB-9ypy: Ribosome with accommodated A-site tRNA, Structure V 手法: EM (単粒子) / 解像度: 3.0 Å 73315 9ypz EMDB-73315, PDB-9ypz: Vacant ribosome with P-site tRNA, substate 1, Structure Ia* 手法: EM (単粒子) / 解像度: 2.9 Å 73316 9yq0 EMDB-73316, PDB-9yq0: Vacant ribosome with P-site tRNA, substate 2, Structure Ib 手法: EM (単粒子) / 解像度: 2.9 Å 73317 9yq1 EMDB-73317, PDB-9yq1: Vacant ribosome with P-site tRNA, substate 3, Structure Ic 手法: EM (単粒子) / 解像度: 2.9 Å
化合物	ChemComp-SPD: SPERMIDINE / スペルミジン ChemComp-ZN: Unknown entry ChemComp-GTP: GUANOSINE-5'-TRIPHOSPHATE / GTP / GTP, エネルギー貯蔵分子YM ChemComp-PHE: PHENYLALANINE / フェニルアラニン ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM ChemComp-MET: METHIONINE / メチオニン ChemComp-K: Unknown entry / カリウムカチオン ChemComp-GCP: PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER / グアニリルメチレンジホスホン酸 / GMP-PCP, エネルギー貯蔵分子類似体YM ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-GDP: GUANOSINE-5'-DIPHOSPHATE / GDP / GDP, エネルギー貯蔵分子YM
由来	oryctolagus cuniculus (ウサギ) saccharomyces cerevisiae (パン酵母) homo sapiens (ヒト)
キーワード	RIBOSOME / GTPBP1 / tRNA / GCP / complex