EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	The structure of phycobilisome with a bicylindrical core from the cyanobacterium Synechococcus elongatus PCC 7942.
ジャーナル・号・ページ	Photosynth Res, Vol. 163, Issue 6, Page 65, Year 2025
掲載日	2025年12月8日
著者	Zhenggao Zheng / Chengying Ma / Hongrui Wang / Guopeng Wang / Chunxia Dong / Ning Gao / Jindong Zhao /
PubMed 要旨	Phycobilisomes (PBSs) are the major light-harvesting complexes in the cyanobacteria and red algae and they consist of a central core and peripheral rods that are attached to the core. The PBS cores ...Phycobilisomes (PBSs) are the major light-harvesting complexes in the cyanobacteria and red algae and they consist of a central core and peripheral rods that are attached to the core. The PBS cores contain 2-5 allophycocyanin cylinders that are organized by ApcE. At the present, structures of PBS with tricylindrical and pentacylindrical cores have been determined while the structure of the PBS with a bicylindrical core is yet to be revealed. Here we report the cryo-EM structure of PBS with bicylindrical core from Synechococcus elongatus PCC 7942 (Synechococcus 7942) at an overall resolution of approximately 3 Å. Similar to the PBS with a tricylindrical core, six peripheral rods are attached to the core by the rod-core linker protein CpcG in the PBS of Synechococcus 7942 even though the core lacks the top AP cylinder, which is important for the attachment of peripheral rods to the tricylindrical cores. We found that the C-terminus of ApcE in the Synechococcus 7942 was involved in interacting with both CpcG and CpcB of a top peripheral rod, compensating for the absence of the top AP cylinder of the core and maintaining PBS stability. Analysis of the bilin distribution reveals that distance of excitation energy transfer from top peripheral rods to the terminal emitters is approximately 15% shorter compared to the PBS with tricylindrical cores. Although there are 30% fewer bilin chromophores in the Synechococcus 7942 PBS core compared with the tricylindrical core, the aromatic residue ring in the Synechococcus 7942 PBS core is conserved, supporting the suggestion that these aromatic residues from AP and linker proteins are critical to the energy transfer of PBS.
リンク	Photosynth Res / PubMed:41359300
手法	EM (単粒子)
解像度	2.79 - 3.99 Å
構造データ	65950 9wg7 EMDB-65950, PDB-9wg7: The structure of phycobilisome with a bicylindrical core from the cyanobacterium Synechococcus elongatus PCC 7942 手法: EM (単粒子) / 解像度: 3.2 Å EMDB-66291: The cryo-EM structure of PBS rod from synechococous elongatus PCC 7942 手法: EM (単粒子) / 解像度: 3.75 Å EMDB-66294: The cryo-EM structure of PBS core from Synechococcus elongatus PCC 7942 手法: EM (単粒子) / 解像度: 2.79 Å EMDB-66296: The cryo-EM structure of PBS from Synechococcus elongatus PCC 7942 手法: EM (単粒子) / 解像度: 3.99 Å 66300 9x6d EMDB-66300: The structure of phycobilisome rod from Synechococcus elongatus PCC 7942 PDB-9x6d: The cryo-EM structure of phycobilisome rod from Synechococcus elongatus PCC 7942 手法: EM (単粒子) / 解像度: 2.92 Å EMDB-66303: The cryo-EM structure of PBS core from Synechococcus elongatus PCC 7942 手法: EM (単粒子) / 解像度: 2.79 Å 66304 9x69 EMDB-66304: The cryo-EM structure of PBS from Synechococcus elongatus PCC 7942 PDB-9x69: The structure of phycobilisome with a bicylindrical core from the cyanobacterium Synechococcus elongatus PCC 7942 手法: EM (単粒子) / 解像度: 2.98 Å
化合物	ChemComp-CYC: PHYCOCYANOBILIN
由来	synechococcus elongatus pcc 7942 = fachb-805 (バクテリア) 7942 (バクテリア)
キーワード	ELECTRON TRANSPORT / PBS / phycobilisome PBS