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Structure paper

TitlePre-splicing conformation and stepwise circularization of a group I intron in Azoarcus pre-tRNA
Journal, issue, pagesNat Commun, Year 2026
Publish dateMay 28, 2025 (structure data deposition date)
AuthorsHong, Y. / Liu, J. / Zhang, X. / Li, S. / Zhang, K.
External linksNat Commun / Search PubMed
MethodsEM (single particle)
Resolution2.86 - 3.27 Å
Structure data

PDB-9v7n:
Cryo-EM structures of the Azoarcus pre-tRNA(ILE) conformation a undergoing the first-step self-splicing
Method: ELECTRON MICROSCOPY / Resolution: 3.11 Å

PDB-9v7o:
Cryo-EM structures of the Azoarcus pre-tRNA(ILE) conformation b undergoing the first-step self-splicing
Method: ELECTRON MICROSCOPY / Resolution: 3.06 Å

PDB-9v7p:
Cryo-EM structures of the Azoarcus pre-tRNA(ILE) intron conformation after the self-splicing
Method: ELECTRON MICROSCOPY / Resolution: 3.01 Å

PDB-9v7q:
Cryo-EM structures of the Azoarcus pre-tRNA(ILE) intron conformation after the cyclization
Method: ELECTRON MICROSCOPY / Resolution: 3.27 Å

PDB-9x6b:
Cryo-EM structure of the Azoarcus sp. BH72 pre-tRNA(ILE) intron after second-step cyclization (circular form)
Method: ELECTRON MICROSCOPY / Resolution: 2.86 Å

Chemicals

ChemComp-GMP:
GUANOSINE

ChemComp-MG:
Unknown entry

Source
  • azoarcus olearius (bacteria)
KeywordsRNA / Azoarcus pre-tRNA(ILE) / self-splicing / pre-1S / RNA structure / circular intron / Azoarcus sp. BH72 pre-tRNA(ILE); second-step cyclization; c' intron

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