EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	A carboxylate switch point controls long-range energy transduction in respiratory Complex I.
ジャーナル・号・ページ	Nat Commun, Vol. 17, Issue 1, Year 2026
掲載日	2026年7月1日
著者	Adel Beghiah / Patricia Saura / Terezia Kovalova / Franziska Hoeser / Thorsten Friedrich / Ville R I Kaila /
PubMed 要旨	Complex I is a highly intricate membrane-bound protein complex that powers the cellular energy metabolism by a long-range ( > 300 Å) proton-coupled electron transfer (PCET) reaction. Here, we ...Complex I is a highly intricate membrane-bound protein complex that powers the cellular energy metabolism by a long-range ( > 300 Å) proton-coupled electron transfer (PCET) reaction. Here, we investigate the highly debated coupling mechanism of Complex I by probing the charge transfer reaction along its functionally central carboxylate pathway (E-channel). By combining biophysical and site-directed mutagenesis experiments with high-resolution (2.6-2.8 Å) cryo-electron microscopy (cryo-EM) and multiscale simulations, we identify a conserved carboxylate switch point (D79) that mediates proton transfer by establishing a kinetic gate and couples the redox chemistry to proton pumping. We find that mutation of the identified site, as found in patients suffering from severe neurodegenerative disorders, drastically perturbs the charge transfer mechanism, and results in a 20% PCET activity. Our combined findings illustrate mechanistic principles of molecular gates underlying long-range charge transfer reactions, and show how disease mutations perturb the function of conserved switch points in energy transduction.
リンク	Nat Commun / PubMed:42386739 / PubMed Central
手法	EM (単粒子)
解像度	2.59 - 3.0 Å
構造データ	55748 9taj EMDB-55748, PDB-9taj: E. coli Complex I WT purified in LMNG 手法: EM (単粒子) / 解像度: 3.0 Å 55749 9tak EMDB-55749, PDB-9tak: Local refinement of E. coli Complex I WT membrane domain in LMNG 手法: EM (単粒子) / 解像度: 2.8 Å 55750 9tal EMDB-55750, PDB-9tal: Local refinement of E. coli Complex I WT hydrophilic domain in LMNG 手法: EM (単粒子) / 解像度: 2.66 Å 55751 9tam EMDB-55751, PDB-9tam: E. coli Complex I D79N NuoA mutant purified in LMNG 手法: EM (単粒子) / 解像度: 2.96 Å 55752 9tan EMDB-55752, PDB-9tan: Local refinement of E. coli Complex I D79N NuoA mutant hydrophilic domain in LMNG 手法: EM (単粒子) / 解像度: 2.59 Å 55753 9tao EMDB-55753, PDB-9tao: Local refinement of E. coli Complex I D79N NuoA mutant membrane domain in LMNG 手法: EM (単粒子) / 解像度: 2.61 Å
化合物	ChemComp-FES: FE2/S2 (INORGANIC) CLUSTER ChemComp-CA: Unknown entry ChemComp-FMN: FLAVIN MONONUCLEOTIDE ChemComp-SF4: IRON/SULFUR CLUSTER ChemComp-3PE: 1,2-Distearoyl-sn-glycerophosphoethanolamine / リン脂質YM ChemComp-LFA: EICOSANE ChemComp-CDL: CARDIOLIPIN / リン脂質YM ChemComp-TRD: TRIDECANE ChemComp-UQ8: Ubiquinone-8 ChemComp-HOH: WATER ChemComp-7PH: (1R)-2-(dodecanoyloxy)-1-[(phosphonooxy)methyl]ethyl tetradecanoate
由来	escherichia coli bw25113 (大腸菌) escherichia coli bl21(de3) (大腸菌)
キーワード	PROTON TRANSPORT / bioenergetics / Leigh's syndrome