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TitleCryoEM Strategies for Elucidating the Closed State Architecture of CCT.
Journal, issue, pagesMethods Mol Biol, Vol. 3070, Page 263-282, Year 2026
Publish dateSep 2, 2026
AuthorsJorge Gutiérrez-Seijo / Ana Cuervo / Sergio Pipaón / David Gil-Cantero / Carmen Majano / César Santiago / José María Valpuesta / Jorge Cuéllar /
PubMed AbstractCryo-electron microscopy (CryoEM) has emerged as a state-of-the-art technique in structural biology, thanks to advances in sample preparation, high-voltage electron microscopy, direct electron ...Cryo-electron microscopy (CryoEM) has emerged as a state-of-the-art technique in structural biology, thanks to advances in sample preparation, high-voltage electron microscopy, direct electron detectors, and sophisticated image processing software. In this study, we investigate the eukaryotic chaperonin CCT, a ~1 MDa hetero-oligomeric complex essential for the folding of key substrates such as actin, tubulin, and WD40 family members. While several open-state structures of CCT have been resolved, the highest-resolution reconstructions have been obtained in the closed state. This is primarily due to the increased structural rigidity of the complex when its apical domains become immobilized in this conformation. However, the pseudo-symmetric arrangement of subunits in the closed state poses challenges for subunit identification. Here, we present a workflow for obtaining a high-resolution CryoEM structure of closed CCT, achieved without the use of nanobodies, substrates, or any tools designed to increase CCT asymmetry. Our results demonstrate that subunit assignment and atomic modeling are achievable by exploiting subunit-intrinsic structural features alone.
External linksMethods Mol Biol / PubMed:42681018
MethodsEM (single particle)
Resolution2.71 Å
Structure data

54634

9s72

EMDB-54634, PDB-9s72:
Human CCT in Closed Conformation
Method: EM (single particle) / Resolution: 2.71 Å

Chemicals

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM

ChemComp-MG:
Unknown entry

ChemComp-AF3:
ALUMINUM FLUORIDE

ChemComp-HOH:
WATER

Source
  • homo sapiens (human)
KeywordsCHAPERONE / Chaperonins / Cryoelectron Microscopy / Closed conformation

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