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TitleThe structure of the bacterial outer membrane transporter FusA enabled by addition of the native lipid lipopolysaccharide.
Journal, issue, pagesJ Struct Biol X, Vol. 12, Page 100141, Year 2025
Publish dateNov 18, 2025
AuthorsJonathan M Machin / Khedidja Mosbahi / Dheeraj Prakaash / Sheena E Radford / Daniel Walker / Antreas C Kalli / Neil A Ranson /
PubMed AbstractLipopolysaccharide (LPS) is a glycolipid found uniquely in the outer membrane of diderm bacteria, formed of 4-7 acyl chains covalently linked to an extended polysaccharide chain. While a few examples ...Lipopolysaccharide (LPS) is a glycolipid found uniquely in the outer membrane of diderm bacteria, formed of 4-7 acyl chains covalently linked to an extended polysaccharide chain. While a few examples of the interaction between LPS and outer membrane proteins (OMPs) have been structurally characterised, either experimentally or computationally, the precise nature of LPS-OMP interactions and their functional consequences remains unclear. Here, we show that the addition of LPS facilitated cryoEM structure determination of FusA, a 100 kDa TonB-dependent outer membrane transporter from . A 2.8 Å structure combined with molecular dynamics of FusA with different LPS models reveals LPS binding sites with a strong LPS interaction site located adjacent to the β-seam region of the FusA β-barrel. The requirement of lipid binding for successful structure determination indicates a stabilisation of the protein, which in turn suggests a potential method for solving other, small OMPs and membrane proteins. Further, it hints at how LPS may mediate protein conformation and thus how LPS and OMPs can work in concert to maintain a structural and functional OM.
External linksJ Struct Biol X / PubMed:41399487 / PubMed Central
MethodsEM (single particle)
Resolution2.4 Å
Structure data

53974

9rhr

EMDB-53974, PDB-9rhr:
FusA (ferredoxin receptor from Pectobacterium atrosepticum) in the presence of Ra-LPS
Method: EM (single particle) / Resolution: 2.4 Å

Source
  • pectobacterium atrosepticum scri1043 (bacteria)
KeywordsMEMBRANE PROTEIN / outer membrane protein / transmembrane beta-barrel / tonb-dependent transporter

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