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TitleA Chemoproteomic Biotechnological Toolkit for Resolving Xylanase Specificity in Decorated Xylan
Journal, issue, pagesTo Be Published
Publish dateJun 2, 2025 (structure data deposition date)
AuthorsCorrea, T.L.R. / Li, Z. / Moroz, O.V. / Pickles, I.B. / Lebedev, A.A. / Akkad, S. / Willems, L. / Overkleeft, H.S. / Davies, G.J.
External linksSearch PubMed
MethodsX-ray diffraction
Resolution1.5 - 2.02 Å
Structure data

PDB-9rdg:
Glucuronoxylan-specific GH30_8 family xylanase CtXyn30A from Clostridium thermocellum complex with glucuronic acid epoxide inhibitor
Method: X-RAY DIFFRACTION / Resolution: 1.5 Å

PDB-9rhv:
GH10 family xylanase XynA from Bacillus sp. KW1 complex with xylobiosyl-configured cyclophellitol probe bearing an alpha-1,3 - Araf decoration
Method: X-RAY DIFFRACTION / Resolution: 2.02 Å

Chemicals

ChemComp-HQ8:
(1~{R},2~{S},4~{S},5~{R})-cyclohexane-1,2,3,4,5-pentol

ChemComp-HOH:
WATER

PDB-1j01:
Crystal Structure Of The Xylanase Cex With Xylobiose-Derived Inhibitor Isofagomine lactam

Source
  • acetivibrio thermocellus dsm 2360 (bacteria)
  • bacillus sp. (in: firmicutes) (bacteria)
KeywordsHYDROLASE / activity-based probes / glucuronoxylan-specific xylanases / GH30_8 / covalent labelling / GH10 family xylanase / arabinoxylan-specific xylanase

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