Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Structural characterization of an extracellular contractile injection system from Photorhabdus luminescens in extended and contracted states.
Journal, issue, pages	Nat Commun, Vol. 16, Issue 1, Page 9327, Year 2025
Publish date	Oct 22, 2025
Authors	Leyre Marín-Arraiza / Aritz Roa-Eguiara / Tillmann Pape / Nicholas Sofos / Ivo Alexander Hendriks / Michael Lund Nielsen / Eva Maria Steiner-Rebrova / Nicholas M I Taylor /
PubMed Abstract	Contractile injection systems (CISs) are phage tail-like nanosyringes that mediate bacterial interactions by puncturing target cell membranes. Within these systems, Photorhabdus Virulence Cassettes ...Contractile injection systems (CISs) are phage tail-like nanosyringes that mediate bacterial interactions by puncturing target cell membranes. Within these systems, Photorhabdus Virulence Cassettes (PVCs) can translocate toxins across eukaryotic target cell membranes and have been engineered to deliver diverse protein cargoes into non-natively-targeted organisms. Despite the structural insights into several CISs, including one PVC from P. asymbiotica, information on PVCs from other species and details on the contraction mechanism remain limited. Here, we present the single-particle cryo-electron microscopy structure of PlPVC1, a PVC from the nematode symbiont and insect pathogen Photorhabdus luminescens DJC, in both extended and contracted states. This particle displays distinct structural features that differ from other CISs, such as a cage surrounding the central spike, a larger sheath adaptor, and a plug exposed to the tube lumen. Moreover, we present the structures of the PlPVC1 fiber and the baseplate of the contracted particle, yielding insight into the contraction mechanism. This study provides structural details of the extended and contracted states of the PlPVC1 particle and supports the model in which contraction is triggered. Furthermore, it facilitates the comparison of PlPVC1 with other CISs and expands the scope of engineering opportunities for future biomedical and biotechnological applications.
External links	Nat Commun / PubMed:41125615 / PubMed Central
Methods	EM (single particle)
Resolution	2.46 - 10.0 Å
Structure data	53137 9qgl EMDB-53137, PDB-9qgl: Cryo-EM structure of the PlPVC1 baseplate, 6-fold symmetrized (C6), in extended state Method: EM (single particle) / Resolution: 2.68 Å 53138 9qgm EMDB-53138, PDB-9qgm: Cryo-EM structure of the PlPVC1 central spike, 3-fold symmetrized (C3), in extended state Method: EM (single particle) / Resolution: 2.82 Å 53139 9qgn EMDB-53139, PDB-9qgn: Cryo-EM structure of the PlPVC1 cap, 6-fold symmetrized (C6), in extended state Method: EM (single particle) / Resolution: 2.46 Å 53140 9qgo EMDB-53140: Cryo-EM structure of the PlPVC1 fiber in extended state PDB-9qgo: Interaction between PlPVC1 baseplate and fiber in extended state Method: EM (single particle) / Resolution: 6.0 Å 53141 9qgp EMDB-53141, PDB-9qgp: Cryo-EM structure of the PlPVC1 sheath, 6-fold symmetrized (C6), in contracted state Method: EM (single particle) / Resolution: 3.1 Å EMDB-53143: Cryo-EM structure of the PlPVC1 baseplate, 6-fold symmetrized (C6), in contracted state Method: EM (single particle) / Resolution: 10.0 Å
Source	photorhabdus luminescens (bacteria)
Keywords	VIRUS LIKE PARTICLE / Contractile injection system