Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Mycobacterial respiratory chain enzymes and growth are inhibited by decylubiquinone.
Journal, issue, pages	Commun Biol, Vol. 9, Issue 1, Page 43, Year 2025
Publish date	Dec 10, 2025
Authors	Sylwia Król / Terezia Kovalova / Mateusz Janczak / Sadaf Kalsum / Mira Akber / Martin Högbom / Susanna Brighenti / Pia Ädelroth / Peter Brzezinski /
PubMed Abstract	Aerobic organisms obtain energy by linking electron transfer from NADH to O, through the respiratory chain, to transmembrane proton translocation. In mycobacteria the respiratory chain is branched; ...Aerobic organisms obtain energy by linking electron transfer from NADH to O, through the respiratory chain, to transmembrane proton translocation. In mycobacteria the respiratory chain is branched; the membrane-bound electron carrier menaquinol (MQH) donates electrons either to the O-reducing cytochrome bd or a supercomplex that is composed of a complex (C) III dimer flanked by two CIVs. Here, we measured the dimethyl-naphthoquinone (DMNQH a menaquinol analogue) oxidation:O reduction activities of the CIIICIV supercomplex and cytochrome bd in the presence of an analogue (decylubiquinone, DCQ) of the mammalian electron carrier, ubiquinol. The data show that DCQH inhibits both the CIIICIV and cytochrome bd activities, suggesting that DCQ/DCQH interferes with both branches of the respiratory chain. Cryo-EM data of the M. smegmatis supercomplex shows that oxidized DCQ binds in the electron donor site (Q) of CIII. Accordingly, growth of M. smegmatis cells was impaired in the presence of DCQ. Remarkably, DCQ also impairs intracellular growth of virulent M. tuberculosis cells in human primary macrophages suggesting that the compound could potentially be used as an adjuvant during tuberculosis disease treatment.
External links	Commun Biol / PubMed:41372535 / PubMed Central
Methods	EM (single particle)
Resolution	2.5 Å
Structure data	53065 9qef EMDB-53065, PDB-9qef: Respiratory supercomplex from Mycobacterium smegmatis with decylubiquinone Method: EM (single particle) / Resolution: 2.5 Å
Chemicals	ChemComp-HEC: HEME C ChemComp-MQ9: MENAQUINONE-9 ChemComp, No image ChemComp-WUO: Unknown entry ChemComp-FES: FE2/S2 (INORGANIC) CLUSTER ChemComp-IZL: [(2~{R})-3-[[(1~{S},2~{R},3~{S},4~{S},5~{R},6~{R})-2-[(2~{R},3~{S},4~{S},5~{S},6~{R})-6-[[(2~{S},3~{S},4~{S},5~{S},6~{R})-6-[[(2~{S},3~{S},4~{S},5~{S},6~{R})-6-(hydroxymethyl)-3-[(2~{R},3~{S},4~{S},5~{S},6~{R})-6-(hydroxymethyl)-3,4,5-tris(oxidanyl)oxan-2-yl]oxy-4,5-bis(oxidanyl)oxan-2-yl]oxymethyl]-3,4,5-tris(oxidanyl)oxan-2-yl]oxymethyl]-3,4,5-tris(oxidanyl)oxan-2-yl]oxy-3,4,5-tris(oxidanyl)-6-[(2~{R},3~{S},4~{S},5~{S},6~{R})-3,4,5-tris(oxidanyl)-6-(undecanoyloxymethyl)oxan-2-yl]oxy-cyclohexyl]oxy-oxidanyl-phosphoryl]oxy-2-undecanoyloxy-propyl] (10~{R})-10-methyldodecanoate ChemComp-9YF: (2R)-2-(hexadecanoyloxy)-3-{[(S)-hydroxy{[(1R,2R,3R,4R,5R,6S)-2,3,4,5,6-pentahydroxycyclohexyl]oxy}phosphoryl]oxy}propyl (9S)-9-methyloctadecanoate ChemComp-7PH: (1R)-2-(dodecanoyloxy)-1-[(phosphonooxy)methyl]ethyl tetradecanoate ChemComp-HEM: PROTOPORPHYRIN IX CONTAINING FE ChemComp-CDL: CARDIOLIPIN / phospholipidYM ChemComp-PLM: PALMITIC ACID ChemComp-9XX: (2S)-1-(hexadecanoyloxy)propan-2-yl (10S)-10-methyloctadecanoate ChemComp-TRD: TRIDECANE ChemComp-3PE: 1,2-Distearoyl-sn-glycerophosphoethanolamine / phospholipidYM ChemComp-HEA: HEME-A ChemComp-CU: COPPER (II) ION ChemComp-MG: Unknown entry ChemComp-CA: Unknown entry ChemComp-DCQ: 2-decyl-5,6-dimethoxy-3-methylcyclohexa-2,5-diene-1,4-dione ChemComp-HOH: WATER
Source	mycolicibacterium smegmatis (bacteria)
Keywords	ELECTRON TRANSPORT / RESPIRATORY SUPERCOMPLEX / MEMBRANE PROTEIN / ACTINOBACTERIA