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-Structure paper
| タイトル | Spatially Aware Diffraction Mapping Enables Fully Autonomous MicroED. |
|---|---|
| ジャーナル・号・ページ | J. Am. Chem. Soc., Vol. 147, Page 42299-42310, Year 2025 |
| 掲載日 | 2025年6月19日 (構造データの登録日) |
著者 | Dmitry B Eremin / Kunal K Jha / David A Delgadillo / Hongyu Zhang / Samuel H Foxman / Samuel N Johnson / Niko W Vlahakis / Duilio Cascio / Vincent Lavallo / Jose A Rodríguez / Hosea M Nelson / ![]() |
| PubMed 要旨 | In the hands of experts, microcrystal electron diffraction (microED, a 3D ED method) is a powerful tool for structural chemistry and chemical discovery. To expand the accessibility and utility of ...In the hands of experts, microcrystal electron diffraction (microED, a 3D ED method) is a powerful tool for structural chemistry and chemical discovery. To expand the accessibility and utility of microED, we introduce Reciprocal Eyes (REyes), an autonomous and intelligent platform (available for academic use) that combines diffraction-based particle selection with real-time data processing to deliver crystal structures without human intervention. REyes spatially maps diffraction signal and autonomously selects crystallites of interest, relying on lattice-quality metrics to acquire and index high-resolution data sets from diverse compounds. Tested on four different transmission electron microscopes (TEMs), it consistently yields preliminary structural solutions from single crystallites of materials, peptides, metal complexes, natural products (NPs), and proteins. |
リンク | J. Am. Chem. Soc. / PubMed:41211926 |
| 手法 | EM (電子線結晶学) |
| 解像度 | 2.2 Å |
| 構造データ | ![]() PDB-9p6r: |
| 化合物 | ![]() ChemComp-CL: ![]() ChemComp-NA: ![]() ChemComp-HOH: |
| 由来 |
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キーワード | HYDROLASE / Ligand / Complex / MicroED |
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