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TitleCryo-EM structure of bacteriophage Bas63 reveals structural conservation and diversity in the genus.
Journal, issue, pagesSci Adv, Vol. 11, Issue 46, Page eadx0790, Year 2025
Publish dateNov 14, 2025
AuthorsJames Hodgkinson-Bean / Rafael Ayala / Klemens McJarrow-Keller / Léna Cassin / Georgia L Rutter / Alexander J M Crowe / Matthias Wolf / Mihnea Bostina /
PubMed AbstractThe BASEL phage collection was developed to provide access to diverse bacteriophages, distinct from model phages. phage JohannRWettstein (Bas63), a myophage in the collection, is a member of the ...The BASEL phage collection was developed to provide access to diverse bacteriophages, distinct from model phages. phage JohannRWettstein (Bas63), a myophage in the collection, is a member of the subfamily Ounavirinae and the genus. Using cryo-electron microscopy, we investigated Bas63's structure to explore its evolutionary relationships and functional adaptations. Our structures reveal a series of gene products: (i) a capsid decorated with β-tulip proteins at three-fold symmetry axes and a Hoc-like protein at hexamer centers, (ii) a conserved connector with an additional 12-fold ring of collar proteins that extend unique whisker proteins that are structurally related to podophage GP4 tail fibers, and (iii) a baseplate with long tail fibers resembling a contracted form of T4's long tail fibers. Sequence conservation analysis of Bas63 structural proteins across ICTV-recognized supports its role as a structural model for evolution. This study advances the mechanistic understanding of phage architecture and reinforces the structural mosaicism of bacteriophages.
External linksSci Adv / PubMed:41223280 / PubMed Central
MethodsEM (single particle) / EM (helical sym.)
Resolution3.0 - 3.17 Å
Structure data

EMDB-48572: C12 local connector reconstruction of phage JohannRWettstein (Bas63)
Method: EM (single particle) / Resolution: 3.0 Å

EMDB-48573: C6 local connector reconstruction of phage JohannRWettstein (Bas63)
Method: EM (single particle) / Resolution: 3.1 Å

48599

9mt4

EMDB-48599, PDB-9mt4:
Connector structure of phage JohannRWettstein (Bas63)
Method: EM (single particle) / Resolution: 3.1 Å

48600

9mt5

EMDB-48600, PDB-9mt5:
Helical tail assembly of phage JohannRWettstein (Bas63)
Method: EM (helical sym.) / Resolution: 3.17 Å

Source
  • escherichia phage johannrwettstein (virus)
KeywordsVIRAL PROTEIN / connector / portal / adaptor / head completion / tail terminator / fiber / whisker / collar / tube / sheath / phage / bacteriophage / neck / Bas63 / JohannRWettstein / Tail

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