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TitleCross-neutralizing and potent human monoclonal antibodies against historical and emerging H5Nx influenza viruses.
Journal, issue, pagesNat Microbiol, Vol. 10, Issue 11, Page 2903-2918, Year 2025
Publish dateOct 14, 2025
AuthorsAlexandra A Abu-Shmais / Gray Freeman / Adrian Creanga / Matthew J Vukovich / Tek Malla / Grace E Mantus / Geoffrey D Shimberg / Rebecca A Gillespie / Vanessa Guerra Canedo / Bernadeta Dadonaite / Megan D Rodgers / Ankita J Chopde / Elizabeth Bardwil-Lugones / Tatsiana Bylund / Amy R Henry / Jesmine Roberts-Torres / Timothy S Johnston / Sarah Smith / Eun Sung Yang / Cheng Cheng / Emma L Walker / Michelle Ravichandran / Ingelise J Gordon / Tejaswi S Dittakavi / Douglas S Reed / Theodore C Pierson / Lesia Dropulic / Jesse D Bloom / Yaroslav Tsybovsky / Eli A Boritz / Daniel C Douek / Tongqing Zhou / Masaru Kanekiyo / Sarah F Andrews /
PubMed AbstractHighly pathogenic avian influenza H5Nx viruses are an emerging threat for global health, especially clade 2.3.4.4b H5N1 virus which causes panzootic infections. Here we describe the isolation and ...Highly pathogenic avian influenza H5Nx viruses are an emerging threat for global health, especially clade 2.3.4.4b H5N1 virus which causes panzootic infections. Here we describe the isolation and characterization of broadly cross-neutralizing monoclonal antibodies (mAbs) against diverse H5Nx viruses from individuals who received a monovalent H5N1 vaccine 15 years ago. By screening over 500 mAbs, we identified 5 mAbs that neutralized the majority of H5 clades including 2.3.4.4b and target three distinct conserved epitopes within the HA globular head. Cryo-electron microscopy structures of these mAbs in complex with HA, deep mutational scanning and neutralization escape studies define the sites of vulnerability of H5 HA. These mAbs mediated stronger prophylactic protection against clade 2.3.4.4b H5N1 infection in mice than the best-in-class mAb targeting the HA stem. Our study identified several highly potent broadly neutralizing H5 mAbs from humans that either alone or in combination provide a pragmatic pandemic preparedness option against the threat of panzootic H5N1 influenza.
External linksNat Microbiol / PubMed:41087744 / PubMed Central
MethodsEM (single particle)
Resolution3.22 - 3.73 Å
Structure data

48515

9mq7

EMDB-48515, PDB-9mq7:
Cryo-EM structure of hemagglutinin H5N1 in complex with Fab 326-366.26
Method: EM (single particle) / Resolution: 3.63 Å

48516

9mq8

EMDB-48516, PDB-9mq8:
Cryo-EM structure of hemagglutinin H5N1 in complex with Fab 310-33-1_H02
Method: EM (single particle) / Resolution: 3.73 Å

48517

9mq9

EMDB-48517, PDB-9mq9:
Cryo-EM structure of hemagglutinin H5N1 in complex with Fab 326-289.74
Method: EM (single particle) / Resolution: 3.38 Å

48518

9mqa

EMDB-48518, PDB-9mqa:
Cryo-EM structure of hemagglutinin H5N1 in complex with Fab 310-7D11
Method: EM (single particle) / Resolution: 3.22 Å

48521

9mqd

EMDB-48521, PDB-9mqd:
Cryo-EM structure of hemagglutinin H5N1 in complex with Fab 310-12D03
Method: EM (single particle) / Resolution: 3.22 Å

Source
  • influenza a virus
  • homo sapiens (human)
KeywordsVIRAL PROTEIN/IMMUNE SYSTEM / Highly pathogenic avian influenza / hemagglutinin H5N1 / bird flu / VIRAL PROTEIN / VIRAL PROTEIN-IMMUNE SYSTEM complex

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