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Structure paper

TitleStructural insights into gibberellin-mediated DELLA protein degradation.
Journal, issue, pagesMol Plant, Vol. 18, Issue 7, Page 1210-1221, Year 2025
Publish dateJul 7, 2025
AuthorsSoyaab Islam / Kunwoong Park / Jing Xia / Eunju Kwon / Dong Young Kim /
PubMed AbstractGibberellin promotes plant growth by downregulating DELLA proteins, which act as growth repressors. In the presence of gibberellin, the gibberellin receptor GID1 binds DELLA proteins, triggering ...Gibberellin promotes plant growth by downregulating DELLA proteins, which act as growth repressors. In the presence of gibberellin, the gibberellin receptor GID1 binds DELLA proteins, triggering their degradation through polyubiquitination by the SCF ubiquitin E3 ligase. Despite extensive studies, the molecular mechanisms by which DELLA proteins assemble with SCF to regulate plant growth remain poorly understood. Here, we present two cryo-electron microscopy structures of the Arabidopsis thaliana DELLA protein RGA in complex with GID1A and GID1A-SLY1-ASK2, respectively. Structural analyses revealed that RGA interacts with GID1A and SLY1 through nonoverlapping binding surfaces, stabilizing the proteins. This suggests that the SCF-RGA-GID1A complex assembles through a stepwise stabilization process induced by gibberellin. Furthermore, structural comparison with GRAS proteins indicates that RGA does not interact with IDD-family transcription factors when bound to SLY1, suggesting that DELLA protein binding to GID1/SLY1 and to transcription factors is mutually exclusive. These findings provide new insights into the gibberellin-mediated regulation of transcription factor activity by DELLA proteins.
External linksMol Plant / PubMed:40542507
MethodsEM (single particle)
Resolution2.66 - 3.07 Å
Structure data

63398

9lum

EMDB-63398, PDB-9lum:
Cryo-EM structure of Arabidopsis thaliana RGA in complex with GID1A
Method: EM (single particle) / Resolution: 2.66 Å

63399

9lun

EMDB-63399, PDB-9lun:
Cryo-EM structure of Arabidopsis thaliana RGA in complex with GID1A, SLY1, and ASK2 (consensus map)
Method: EM (single particle) / Resolution: 2.8 Å

63400

9luo

EMDB-63400, PDB-9luo:
Cryo-EM structure of Arabidopsis thaliana RGA in complex with GID1A, SLY1, and ASK2 (focused map)
Method: EM (single particle) / Resolution: 3.07 Å

63401

9lup

EMDB-63401, PDB-9lup:
Cryo-EM structure of Arabidopsis thaliana RGA in complex with GID1A, SLY1, and ASK2 (composite map)
Method: EM (single particle) / Resolution: 2.8 Å

Chemicals

ChemComp-GA3:
GIBBERELLIN A3

ChemComp-HOH:
WATER

Source
  • arabidopsis thaliana (thale cress)
KeywordsHORMONE / Gibberellin / DELLA motif / GRAS domain / Plant growth

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