EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Cradle loop regulates β-barrel pore-formation mechanism of Vibrio cholerae cytolysin.
ジャーナル・号・ページ	Structure, Vol. 34, Issue 1, Page 157-174.e6, Year 2026
掲載日	2026年1月8日
著者	Mahendra Singh / Arnab Chatterjee / Ananya Nayak / Prasenjit Naskar / Gurvinder Kaur / Jagannath Mondal / Somnath Dutta / Kausik Chattopadhyay /
PubMed 要旨	Vibrio cholerae cytolysin (VCC) is a β-barrel pore-forming toxin (β-PFT). The membrane insertion of its pore-forming "pre-stem" motif is the most crucial step in the pore-formation mechanism. In ...Vibrio cholerae cytolysin (VCC) is a β-barrel pore-forming toxin (β-PFT). The membrane insertion of its pore-forming "pre-stem" motif is the most crucial step in the pore-formation mechanism. In the soluble monomeric form, pre-stem remains clamped against the central cytolysin domain by the so-called cradle loop. In the course of oligomeric pore-formation in the target membranes, the cradle loop gets detached from the pre-stem and reorients, thus allowing the pre-stem to extend and insert into the membrane. Here, we show that the specific cradle loop residue(s) play crucial roles in governing the pore-formation mechanism of VCC by establishing decisive interactions with the neighboring structural domains/modules. The alteration of the cradle loop residue, Y194 in particular, compromises the membrane-insertion of the pre-stem, and tends to arrest the membrane-bound toxin in the pre-pore-like oligomeric states. Our study suggests that the native cradle loop architecture, with its intact contacts with the surrounding interaction partners, is essential for VCC pore-formation.
リンク	Structure / PubMed:41202807
手法	EM (単粒子)
解像度	3.51 - 6.57 Å
構造データ	EMDB-63070: Pre-pore state (without transmembrane barrel) of Y194A Vibrio cholerae cytolysin mutant in liposome. 手法: EM (単粒子) / 解像度: 6.57 Å EMDB-63073: Pre-pore octameric state of T201A Vibrio cholerae cytolysin mutant in liposome 手法: EM (単粒子) / 解像度: 4.76 Å 63083 9lh3 EMDB-63083: Half barrel Pre-pore state of Y194A Vibrio cholerae cytolysin mutant in liposome. PDB-9lh3: Vibrio cholerae cytolysin cradle loop mutant-Y194A half barrel pre-pore model 手法: EM (単粒子) / 解像度: 3.51 Å 63088 9lh7 EMDB-63088, PDB-9lh7: Pore-like heptameric T201A Vibrio cholerae cytolysin mutant 手法: EM (単粒子) / 解像度: 3.76 Å PDB-9ue9: Heptameric pore structure of Vibrio cholerae Cytolysin (VCC) embedded in lipid bilayer 手法: ELECTRON MICROSCOPY / 解像度: 4 Å
化合物	ChemComp-HOH: WATER
由来	vibrio cholerae (コレラ菌)
キーワード	TOXIN / Membrane Protein / Heptameric Half-barrel Prepore / VCC mutant / Pore-forming toxin / Heptameric Pore-like / Vibrio cholerae Cytolysin (VCC)